Indulge in The Ocean’s Beauty with Our Dolabellanin Manufacture
CD BioGlyco proudly presents our Marine Biomolecule Production Service, with a focus on Glycoproteins such as dolabellanin. Dolabellanin is a biomolecule that is extracted from the sea hare Dolabella auricularia. The organism, found in the Indian and Pacific Oceans, is a substantial shell-less opisthobranch mollusk that thrives intertidally while consuming brown algae. Certain D. auricularia contains cytotoxic and/or antineoplastic compounds, which are small molecules primarily sourced from algal diets. CD BioGlyco produces five kinds of dolabellanins, dolabellanin A, dolabellanin B, dolabellanin C, dolabellanin E, and dolabellanin P, from different parts of D. auricularia, including the coelomic fluid, the purple fluid, the albumen grand, and egg mass.
Collection of D. auricularia and its egg mass
We stimulate live D. auricularia to secrete purple liquid and then separate the coelomic body fluid and the organs from the animals and freeze at -80°C until use. According to clients’ demand, take corresponding part in the extraction process.
Extraction
- We will slice the frozen body-wall bricks and homogenize them with buffer.
- After centrifuging for several minutes, collect the supernatant.
- Next, the precipitate will be treated with acetonitrile and trifluoroacetic acid in water, and centrifuge it again under the same conditions.
- The resulting second supernatant will be lyophilized and dissolved in water.
- To further purify the supernatant, we conduct ultrafiltration using a centrifugal membrane filter device.
Purification
Subsequently, we use two key techniques, gel filtration and high-performance liquid chromatography (HPLC), for the purification process. Gel filtration and HPLC provide a powerful combination for purifying and analyzing compounds, with gel filtration being particularly useful for initial purification based on size, followed by HPLC for further separation and analysis based on specific interactions with the stationary phase.
Characterization
We utilize mass spectrometry (MS) as a powerful tool for the determination of glycoproteins, attributed to its remarkable sensitivity, stoichiometry, specificity, and speed. This capability greatly aids in the identification of glycoproteins, glycosylation sites, and the structural elucidation of glycans.
Publication Data
Technology: Ultrafiltration, Reversed-phase HPLC (RP-HPLC), Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF), Molecular dynamics simulation
Journal: Biosciences Biotechnology Research Asia
Published: 2015
IF: 4.268
Results: In the study, authors explored protein extraction from a marine gastropod, Peronia peronei, residing in the northern region of the Persian Gulf. They conducted a comprehensive investigation by utilizing advanced techniques including ultrafiltration, RP-HPLC, and MALDI-TOF/TOF. Spectral analysis unveiled the presence of a potentially significant peptide, dolabellanin B2, previously obtained from another marine mollusk named D. auricularia. They employed molecular dynamics simulation and antibacterial assays to unveil the spatial structure and potential mechanism of action of the previously identified natural antimicrobial peptide, dolabelanin-b2. Molecular characteristics, including secondary structure composition, surface hydrophobicity, and distribution of electrostatic potential, indicate a possible link between this peptide and alpha-helical cationic antimicrobial peptides. The biophysical examination of peptides through computational and experimental approaches may illuminate the precise roles of various parameters, such as net charge, percentage of α-helical/β-sheet content, and amphipathicity, in the efficacy and mode of action of antimicrobial agents. Subsequent evaluation of the purified peptide’s antibacterial properties was performed against a spectrum of bacterial strains, including Staphylococcus aureus, Bacillus subtilis, Serratia marcescens, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Proteus mirabilis. Several methodologies including the broth micro-dilution approach, standard disk diffusion assay, and bio-autography were employed to assess its efficacy. The results conclusively demonstrated a potent antibacterial activity of the identified peptide against all aforementioned bacterial strains. The study of marine invertebrate antimicrobial peptides, in conjunction with molecular dynamics investigations, necessitates further endeavors and holds promise for a wide spectrum of investigations into novel natural antibiotics.
Applications
- Dolabellanin is utilized in the pharmaceutical industry for its potential antimicrobial properties, showing promise as a natural antibiotic agent.
- Dolabellanin can be used to develop and research anticancer agents due to its antineoplastic properties.
- Dolabellanin may play a role in marine ecosystem dynamics, hinting at its ecological significance.
Advantages
- Our researchers offer a range of comprehensive services beyond glycoprotein production, including glycosylation site analysis, glycan structure determination, and glycoprotein characterization.
- We prioritize client satisfaction, striving to meet and exceed the expectations of clients through personalized service, timely delivery, and ongoing support.
- Our research team is dedicated to innovation and continuously seeks to improve its techniques and offerings in the glycoprotein production industry.
Frequently Asked Questions
What other marine glycoprotein can CD BioGlyco produce?
- Aplysianin
- Pachymatismin
- Foot Protein 1
- Marine Collagen
- Julianin
- Crambescidin
- Mucin-type Glycoprotein
- Limpet Hemocyanin
- Dehydrocrambine A
- Stanniocalcin
Can CD BioGlyco Modify the structure of extracted active substances?
We can confidently say that we can! Our efforts in tailoring marine carbohydrates involve a triadic approach encompassing polymer conjugation, chemical derivatization, and enzymatic transformations. These modifications instigate bespoke alterations in the adsorption kinetics, metal ion complexation, and other physicochemical properties of the carbohydrate moieties.
By various methods, CD BioGlyco provides tailored production solutions for numerous Marine Carbohydrates, ensuring superior quality and personalized modifications. Our commitment to precision and innovation caters to the specific requirements of clients in the research and pharmaceutical fields. Please feel free to contact us for top-tier glycoprotein production services.
Reference
- Bitaab, M.A.; et al. Antibacterial and molecular dynamics study of the dolabellanin B2 isolated from sea slug, Peronia peronii. Biosciences Biotechnology Research Asia. 2015, 12(3): 2023-2035.
