Marine Lectin Production Service

CD BioGlyco possesses a top research team that devotes itself to producing diverse highest-quality lectins from marine organisms. We will also support your research goals with our Marine Biomolecule Production Service, such as Marine Carbohydrate Production Service, Marine Glycoprotein Production Service, and Marine Glycoside Production Service. Lectins play a crucial role in the detection, isolation, and characterization of glycoconjugates both in solution and on the surfaces of cells. This property of lectins makes them valuable tools for studying and understanding the complex world of carbohydrates and their interactions with other molecules. We also utilize marine lectins to identify and study glycoconjugates, aiding in the advancement of research in various areas.

By carefully researching the complex characteristics and structures of lectins, we ensure the production of high-quality marine lectins with diverse biological functions. Through our cutting-edge technologies and extensive expertise, we cultivate and extract lectins from marine sources and purify them to the highest standards. This enables clients to access lectins that play pivotal roles in various applications, such as understanding carbohydrate-protein interactions, developing therapeutics, and unraveling the diverse functions of lectins in biological systems.

Fig.1 Typical crystal structures of fish lectins. (Ahmmed, et al., 2022)

Our marine lectin production service offers a comprehensive solution, enabling clients to harness the potential of lectins in their research and development endeavors. CD BioGlyco provides extraction, purification, and characterization methodologies of marine lectin derived from marine organisms including fish, crustaceans, and molluscs.

• The widely used method of extracting lectins from marine samples is a simple two-step process employing a polar salt solution and purification by column chromatography. Aqueous, acid, and alkaline solution-based methods are usually utilized to isolate marine lectins.
• Subsequently, we conduct a centrifugation process for extract clarification and fractionation and enrichment by ultrafiltration, precipitation, and/or chromatography. To preserve the structure of proteins and their ability to interact, we also use gentle extraction conditions with specific pH ranges.

By delivering lectins with exceptional binding capabilities and biological activities, CD BioGlyco helps clients promote development in fields like biomedicine, agriculture, and biotechnology.

Publication Data

Technology: Ion-exchange chromatography, Fluorescence spectra, Fourier-transform infrared spectroscope (FT-IR)

Journal: Journal of Chemistry

IF: 3.0

Published: 2019

Results: This study discussed the isolation and purification of a novel lectin called LML from loach skin mucus. LML exhibited a high hemagglutination activity of 166.23×103 HU/mg. Ion-exchange chromatography, a conventional protein purification technique, was employed for the purification of LML. The lectin is a glycoprotein with a molecular weight of 245 kDa. Monosaccharide composition analysis revealed that its carbohydrate chain consists of rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 2.02 : 11.66 : 2.06 : 1.00 : 14.09 : 6.00. LML requires Ca²⁺ to induce hemagglutination and is strongly inhibited by D-lactose. It exhibits high alkali resistance, maintaining approximately 30% hemagglutination activity at pH 14.0, while its acid resistance is weak. The maximum hemagglutination activity of LML was observed within a temperature range of 20°C to 50°C. Moreover, the study provided preliminary insights into the structure of LML, revealing abundant glutamic acid, histidine, and serine amino acids. Its secondary structure consists of α-helices (4.97%), β-sheets (27.55%), turn structures (49.78%), and unordered structures (17.70%).

Frequently Asked Questions

What is the origin of the name lectins?

Lectins are so named because they possess the ability to agglutinate red blood cells by binding specifically to their carbohydrate moieties through at least one noncatalytic domain.

What is the role of agglutinin in organisms?

Lectins, which are proteins, have the unique ability to bind to carbohydrates in a specific and reversible manner. They are found in various organisms such as animals, plants, and microorganisms, and primarily function as cell recognition molecules.

What activities do lectins have?

Applications

• Marine lectins have an effect on enhancing the healing process and expediting wound closure and can be used as a potential acute wound healing agent.
• Marine lectins can be used for their inhibitory effect on the development of malarial parasites.
• Due to their ability to enhance the adherence of microparticles to target cells and improve drug penetration, lectins can also be utilized for research on directing therapeutic agents toward specific cells.
• Marine lectins can be used as specific probes for both intracytoplasmic compartments and cell surface glycoconjugates in histopathological and histological applications.

Advantages

• We have advanced techniques and quality control measures to consistently deliver high-quality lectins for diverse research applications.
• We are constantly improving our production methods to obtain abundant marine lectins and other marine biomolecules.
• Our state-of-the-art technologies are not only employed to produce and purity marine lectins but also to analyze their activities.

CD BioGlyco is a lead company that possesses a vast amount of knowledge of unique characteristics and structures of lectins and leverages this knowledge to deliver exceptional products. Contact us today to explore the field of glycobiology together.