Antiinflammatory Activity Screening Service

Antiinflammatory Activity Screening Service at CD BioGlyco

Inflammation is a common physiologic response. However, when inflammation is over-excited or uncontrollable, it will lead to a variety of diseases. Marine glycans & glycoproteins have a unique structure and are highly targeted with low toxicity and good anti-inflammatory activity. CD BioGlyco has extensive experience in researching the Bioactivity of Marine Glycans and Glycoproteins and proposes a comprehensive anti-inflammatory activity screening service through the research of inflammation-producing mechanisms.

We dissolve the marine glycans and glycoproteins to be tested in buffer, and use enzyme-linked immunosorbent assay (ELISA) to detect their IC₅₀ values for inhibiting PGE₂ production in Anal cells and EC₅₀ values for promoting TNF, and IL-12 secretion, and then analyze their anti-inflammatory activities.

The related services we provide include but are not limited to, the following.

• Hemolysis Inhibition Assay

The hemolysis test is an experiment used to detect the release of hemoglobin from ruptured red blood cells under specific conditions. Under the condition of setting up a negative control group, we put the sample to be tested in contact with blood and measured the amount of hemoglobin released from red blood cells by spectrophotometry to detect the hemolysis inhibition effect of the donor sample.

• Proteinase Inhibition Assay

We develop an activity assay method for the proteasome using a fluorescent peptide substrate specific to the proteasome. In addition, we develop a method for analyzing the subunit components of proteases based on proteasome-specific affinity probes. Then, the fluorescence activity of the protease is detected using an enzyme marker, and the components and expression of the protein are analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

• Protein Denaturation Inhibition Assay

The peptide chains in a protein molecule are coiled and twisted in a certain way to form a specific conformation. When a protein undergoes denaturation, the peptide chains stretch out, exposing -SH inside the molecule. Therefore, the change in -SH can be measured to gauge the degree of denaturation and denaturation inhibition of the protein. Adding sodium ferrocyanide to the sample to be tested is a visual method of detecting protein denaturation. The reaction produces a red complex that can be measured colorimetrically.

• Lipoxygenase (LOX) Inhibition Assay

When inflammation occurs, LOX catalyzes the oxidation of unsaturated fatty acids, leading to peroxidation of membrane lipids, and the oxidation products show a characteristic ultraviolet absorption peak at 280 nm. Using an ultraviolet spectrophotometer, we measure the rate of increase in the absorbance at 280 nm of the samples and calculate the activity of LOX. In addition, we set up a control group to evaluate the LOX inhibition ability of the substances to be tested.

Fig.1 Anti-inflammatory activity screening services. (CD BioGlyco)

In addition, the presence or absence of glycan substituents and species differences have an impact on their anti-inflammatory activity. We also offer Modifications to marine glycans that enhance their anti-inflammatory activity.

Publication

Technology: Nuclear magnetic resonance (NMR), In vivo and in vitro experiments

Journal: International Journal of Biological Macromolecules

IF: 8.2

Published: 2020

Results: Fucoidan (LJSF4) has strong anti-inflammatory properties. The monosaccharides of LJSF4 consisted of fucose, galactose, rhamnose, xylose, and mannose with contents of 79.49%, 16.76%, 0.82%, 1.08%, and 1.84%, respectively. The NMR spectra showed that LJSF4 is a polysaccharide characterized by the presence of 1→3-linked α-1-ribofuranosyl and →4-α-1-furandiyl groups alternately on the main chain of the polysaccharide, and the sulfate groups are mainly located in the C-4 and C-2 positions. In vitro, anti-inflammatory experiments indicated that LJSF4 reduced nitric oxide (NO) and cytokine production during inflammation. Its mechanism is related to the down-regulation of the expression of signaling pathways, including MAPK and NF-κB. Through in vivo experiments, LJSF4 had a significant protective effect on Zebrafish exposed to lipopolysaccharide, which reduced cell mortality, NO, and reactive oxygen species production.

Fig.2 Inhibitory effects of LJSF4 on lipopolysaccharide-induced cell death, reactive oxygen species production, and NO production in Zebrafish embryos. (Ni, et al., 2020)

Applications

• Anti-inflammatory activity screening can be used to develop therapeutic agents for inflammation-induced heart disease, multiple sclerosis, and arthritis, among other diseases.
• Screening services of anti-inflammatory activity can be used to model inflammation in vivo and in vitro.
• Different types of marine glycans and glycoproteins have different effects on anti-inflammatory mechanisms. Their screening for anti-inflammatory activity can be used to explore the mechanisms of inflammation-induced diseases.

Advantages

• We customize anti-inflammatory activity screening programs to ensure client satisfaction.
• We have a reliable laboratory team and world-leading bioactivity assay technology to provide high-quality services.
• Our service team provides anti-inflammatory activity screening services from multiple perspectives to target the mechanisms of inflammation and its development.

CD BioGlyco offers a comprehensive marine glycan and glycoprotein anti-inflammatory activity screening service. We have a state-of-the-art laboratory and perform high-quality assays. Please feel free to contact us for more details.