Expanding Carijoside A Production with Our Professional Experience
Carijoside A is a bioactive sterol glycoside with anti-inflammatory activity. Carijoside A is a pure and natural Marine Biomolecule. Through our advanced extraction and purification techniques, carijoside A maximizes its natural activity and provides strong support for your project applications. Whether for experimental research, biopharmaceuticals, or as a feedstock molecule, Carijoside A from CD BioGlyco is the ideal choice for you.
Marine Octocoral Pretreatment
Our biological lab staff rinse the collected coral samples using purified water or seawater to remove sediment and large particles of impurities from the surface. The cleaned coral samples are placed in an ultrasonic cleaner and a detergent is added to remove microorganisms attached to the coral. The coral samples are rinsed several times with purified water to ensure that all disinfectant residue was removed. Finally, our researchers cut the coral into pieces.
Extraction and Isolation of Carijoside A
Our lab provides professional carijoside A extraction service by organic solvent extraction such as MeOH and CH2Cl2. Moreover, we use ethyl acetate and water for multiple extractions of the extracts. The target component is separated by silica gel and eluted using hexane/acetone. Elution gradients and buffers have been optimized by our researchers to ensure optimal separation.
Characterization of Carijoside A
Our commonly used analytical tools include nuclear magnetic resonance (NMR), Fourier-transform infrared spectroscopy (FTIR), electrospray ionization mass spectrometry (ESI-MS), and high-performance liquid chromatography (HPLC). For purified carijoside A, we offer a range of services as follows.
- Physical and chemical property analysis
- Monosaccharide composition analysis
- Structural characterization
- Human neutrophil superoxide anion generation assay
- Elastase release assay
- Cytotoxicity testing
Publication
Technology: High-resolution electrospray ionization mass spectrometry (HR-ESI-MS), IR, NMR, Cytotoxicity testing
Journal: Marine Drugs
Published: 2010
IF: 4.9
Results: The authors purified a novel glycoside, carijoside A, from an octocoral Carijoa sp. The structure of carijoside A was determined after extensive spectroscopic analyses. Moreover, the researchers characterized the biological activity of carijoside A. The results showed that carijoside A inhibits superoxide anion production and elastase release in human neutrophils.
Fig.1 The structure of carijoside A. (Liu, et al., 2010)
Applications
- Antiinflammatory drug development: As a natural product with anti-inflammatory activity, carijoside A is well suited for the development of drugs for the treatment of inflammation-related diseases.
- Biomarker: Carijoside A can be used as a biomarker for specific diseases or physiological states for disease diagnosis and monitoring.
- Science research: Carijoside A is used as a reagent in cell biology and pharmacology studies to investigate cell signaling pathways and metabolic mechanisms.
- Agriculture: Carijoside A plays an important role in the development of inhibitors of plant pathogens.
Advantages of Us
- Fast delivery: With a robust supply chain management system and efficient production capacity, we ensure that our clients get the carijoside A they need on time.
- Quality Assurance: CD BioGlyco has a professional glycoside production team, adopting advanced production technology and a strict quality control system to ensure that the Carijoside A produced has high purity and excellent quality.
- Trusted craftsmanship: From raw material handling, extraction, and separation to characterization, all steps are strictly controlled. The temperatures, extraction concentrations, and buffers involved in the production process are designed and optimized by professionals.
Frequently Asked Questions
What is the structure of carijoside A?
The structure of carijoside A is 3β-O-(3',4'-di-O-acetyl-β-D-arabinopyranosyl)-25ζ-cholestane-3β,5α,6β,26-tetrol-26-acetate.
What other steroid glycosides can be isolated from the soft corals?
The steroid glycoside obtained by isolation and purification from soft coral further comprising hirsutosteroside A (ragnane glycoside), 24-methylenecholest-5-ene-3β,16β-diol-3-O-α-L-fucoside, and sinubrasone A.
CD BioGlyco provides our clients with comprehensive technical support and production services to help them solve all kinds of problems encountered during the use of carijoside A and to ensure the effectiveness of its application. We are committed to being your first choice in carijoside A production. Whether you are interested in carijoside A production, please feel free to contact us.
Reference
- Liu, C.Y.; et al. Carijoside A, a bioactive sterol glycoside from an octocoral Carijoa sp.(Clavulariidae). Marine Drugs. 2010, 8(7): 2014-2020.
