Comprehensive and Customized Chitosan Characterization Service
Chitosan is biocompatible and has excellent biological properties for applications in cosmetics, pharmaceuticals, preservation, food, wastewater treatment, and other fields. As a natural amino polysaccharide, its structure and physicochemical properties vary somewhat depending on the source and extraction method. CD BioGlyco offers a comprehensive and customized chitosan characterization service based on its extensive experience in the Characterization of Marine Animal Polysaccharides.
Relying on the accumulation of excellent technology, we have rich practical experience in chitosan extraction, purification, and characterization. Adhering to the concept of professionalism and dedication, we provide clients with optimal analytical solutions and reliable support for applied research. Combining various analytical methods, we mainly provide the following services:
Extraction and Purification of Chitosan
This process consists of demineralization, deproteination, and deacetylation. We extract chitosan from a variety of crustaceans and prepare it chemically, enzymatically, with ultrasound-assisted extraction, etc. In addition to this, a decolorization process is performed to remove the pigments contained in the samples. Differences in the chitosan preparation process and many parameters in the deacetylation reaction affect the properties of the final chitosan. We optimize the chitosan extraction process to ensure that our client's requirements for chitosan properties are met.
Characterization of Chitosan
We characterize the physicochemical and functional properties of chitosan, including the degree of acetylation, distribution of acetyl groups along the chain, molecular weight, viscosity in solution, etc. Characterization protocols are customized to specific needs and include the following components:
- Molecular weight: We examine molecular weight distribution, average molecular weight, and intrinsic viscosity.
- Degree of acetylation (DA): DA is the proportion of acetylglucosamine units in chitosan and is influenced by various parameters in the deacetylation step. It affects a variety of physicochemical and biological properties of chitosan, such as hydrophilicity, crystallinity, and solubility. Infrared spectroscopy (IR), nuclear magnetic resonance (NMR), etc., are used to analyze the degree of acetylation of chitosan.
- Acetyl group distribution: The distribution of acetyl groups along the chitosan chain is determined by 13C-NMR spectroscopy.
- Crystal structure: X-ray diffraction, NMR, and IR are used to analyze the crystal structure of chitosan.
- Functional groups: Fourier transform infrared spectroscopy (FTIR) is used to determine and analyze the functional groups in chitosan.
- Scanning electron microscope (SEM): We analyze the surface morphology of chitosan by SEM to reveal the details of its microstructure.
- Thermal stability: We analyze the thermal stability of chitosan by differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA).
Chemical Modification of Chitosan
We also provide a chemical modification service to chemically modify the molecular structure of chitosan through various chemical reactions such as alkylation, quaternization, and grafting.
Applicable Samples
To provide you with high-quality service, we need samples should be:
- Raw material for chitosan extraction (drying and pulverizing of fresh samples is recommended first)
- Purified, high-quality chitosan samples (lyophilized powder)
Sample size depends on the specific experimental protocol.
Delivery of Results
We deliver the following results and high-quality chitosan products to our clients.
- Experimental protocols for extraction, purification, and characterization
- Raw data of chitosan characterization
- Detailed analysis of chitosan structure and physicochemical properties
Publication Data
Technology: XRD, FTIR, viscosimetry, SEM
Journal: Marine Drugs
IF: 4.379
Published: 2017
Results: In this study, a new method for the preparation of chitosan from shrimp shells was developed and the structure of chitosan was characterized by viscosimetry, XRD, UV, FTIR, and SEM. The results showed that the extracted chitosan samples were properly characterized, with their acetylation degree below 9%, ash content all below 0.063%, and soluble in 1% acetic acid. The molecular weights of the chitosan samples were found to be in the range of 2.3 and 2.8 × 105 g/mol and the degree of crystallinity was found to be around 40%. The results demonstrate that the extraction method developed in this study is suitable for obtaining chitosan with properties suitable for drug research. The method used in this paper also guides the development of extraction methods and structural characterization of chitosan.
Fig.1 FTIR spectra of chitosan samples. (de Queiroz Antonino, et al., 2017)
Applications
- Chitosan is used to prepare edible antimicrobial membranes with good film-forming properties. Chitosan characterization plays an important role in antimicrobial film development.
- Chitosan characterization helps to analyze its application in the development of materials for wound dressings and tissue engineering.
- Different marine sources and extraction methods affect the structure of chitosan. Its characterization helps to develop and evaluate chitosan extraction methods.
Highlights
- We tailor our characterization methods to meet your chitosan-specific research needs.
- We use state-of-the-art equipment and techniques to obtain accurate and reliable chitosan characterization results.
- Results are thoroughly analyzed and interpreted by our team of experts with extensive knowledge and experience in chitosan characterization.
Frequently Asked Questions
What is the biological significance of chitosan structure and property characterization?
Chitosan has a wide range of applications in biomedicine, materials development, and pharmaceuticals due to its antimicrobial and coagulation activities. It is found that the structure and properties of chitosan such as the degree of deacetylation affect its performance in many applications. Characterization of chitosan structure and properties can help develop efficient production solutions and promote the exploitation of its application value.
Can you design a chitosan characterization protocol based on our research objectives?
Of course, our team has extensive expertise and experience in marine polysaccharide characterization develops tailored protocols based on our client's research needs, and performs detailed analyses.
CD BioGlyco provides a high-quality chitosan characterization service based on many years of technical deposits and analytical experience. As your most reliable partner, please feel free to contact us with your chitosan characterization needs.
Reference
- de Queiroz Antonino, R.S.C.M.; et al. Preparation and characterization of chitosan obtained from shells of shrimp (Litopenaeus vannamei Boone). Marine drugs. 2017, 15(5): 141.
