Structure Analysis of Marine Glycoprotein at CD BioGlyco
CD BioGlyco combines different technical approaches to provide our clients with highly accurate marine glycoprotein analysis services. In addition to Quantitative Analysis, our glycoprotein structural analysis technology is also state-of-the-art. We provide a one-stop Marine Glycoprotein structural analysis service according to the different research needs of our clients for marine glycoproteins.
Fig.1 Process of structural analysis of marine glycoproteins. (CD BioGlyco)
Process of Analysis
Analyzing the structure of marine glycoproteins is an important prerequisite for understanding their biological functions. We perfect the process of analyzing the structure of glycoproteins. We first use protease to cleave specifically modified marine glycoproteins into small fragments of glycopeptides, and then purify and enrich the glycopeptides obtained. Afterward, liquid chromatography-tandem mass spectrometry (LC-MS/MS) or hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS) is utilized for online sampling to obtain raw mass spectral data. Then, the obtained glycopeptide sequences are searched and analyzed based on compositional and structural fingerprints, and site and structure specificity databases. Finally, the structural analysis of marine glycoproteins is realized through the analysis of monosaccharides, molecular weight, amino acid composition, glycopeptide bonds, etc.
We have advanced LC-MS/MS, HILIC-MS/MS, and other high-resolution mass spectrometry systems. These advanced mass spectrometry systems support us in providing highly accurate glycoprotein structure analysis services.
Sample Request
Our marine glycoprotein structure analysis service is used for a wide range of biological samples, including plasma, cells, tissues, and so on. Clients only need to tell the experimental objectives and provide the experimental samples. All subsequent experiments for structural analysis, including protein extraction, enzyme digestion, peptide separation, mass spectrometry analysis, data analysis, etc., are all done by us.
To better develop a detailed plan for our clients, we categorize our marine glycoprotein structure analysis services as follows:
Glycoconjugates have many branching structures and spatial conformations and therefore carry a large amount of biological information. At CD BioGlyco, our glycan profiling of marine glycoproteins includes extraction and isolation of glycoproteins, glycan release, and identification of glycan structures.
Structure identification is critical in the study of marine glycopeptide activity. CD BioGlyco provides high-quality marine glycopeptide analysis services based on advanced mass spectrometry systems.
Glycans play a variety of functional and structural roles in various proteins. CD BioGlyco offers a high-sensitivity released marine glycan analysis service including monosaccharide composition, structure, etc.
The number and type of glycan units bound to proteins affect the biological function of glycoproteins. CD BioGlyco analyzes bound monosaccharides by hydrolytic release and chromatographic separation.
Publication Data
Technology: Fourier transform infrared spectroscopy (FT-IR)
Journal: Food Research International
IF: 8.1
Published: 2023
Results: In this study, a glycoprotein (SGP1) with a molecular weight of 9.26 kDa was isolated from Sipunculus nudus. The structural analysis of this glycoprotein showed that the sugar moiety of SGP1 was mainly composed of glucose, xylose, and lysosaccharides. FT-IR results showed that SGP1 had typical glycoprotein features. It contains O-type glycosidic bonds and is a protein-dominated glycoprotein (92.8% protein).
Fig.2 Extraction, purification, and resolution of SGP1. (Lu, et al., 2023)
Applications
- Glycoprotein structure analysis is used as a basis for studying the effects of glycosylation on protein function. Disease mechanisms are investigated by comparing the differences between normal glycoproteins and diseased proteins.
- Glycoprotein structure analysis helps to gain further insight into the hormone selectivity of glycoprotein hormones, receptor activation mechanisms, etc.
- Glycoprotein structure analysis is used to study cell-cell adhesion, inflammation generation, and more.
Advantages of Us
- We have a series of high-resolution mass spectrometry systems for precise structural analysis of marine glycoproteins.
- We provide a one-stop service for marine glycoprotein structure analysis. Clients only need to tell us their needs and provide samples.
- Our assays are complete, reproducible, and have high accuracy of results.
CD BioGlyco has established a complete glycoprotein analysis program based on high-resolution mass spectrometry systems. With experienced researchers and a quality control system, we will customize the analysis plan according to the client's actual needs. Please feel free to contact us for more information about the sample requirements and the structure analysis process of marine glycoproteins. We look forward to your visit.
Reference
- Lu, X.; et al. Purification and characterization of a glycoprotein from Sipunculus nudus and its immune-enhancing activity to RAW 264.7 macrophages. Food Research International. 2023, 174: 113591.
