Marine Glycoconjugate Characterization Service

Marine Glycoconjugate Characterization Service at CD BioGlyco

Marine glycoconjugates have a wide range of biological activities and medical values. By characterizing the structure and activity of these glycoconjugates, new natural products will be discovered, providing potential sources and candidate molecules for drug development and biotechnological applications. CD BioGlyco focuses on the field of Marine Glycoconjugate Development, which utilizes various advanced technologies to provide professional marine glycoconjugate characterization service.

• Separation and purification service: We provide separation and purification of marine glycoconjugates from different sources and types, which is an important step in studying the biological activity and structure of marine sugars. Commonly used methods include gel filtration chromatography, ion exchange chromatography (IEX), affinity chromatography, high-performance liquid chromatography (HPLC), etc.
• Glycosylation characterization service: We provide efficient characterization for glycans in marine glycoconjugate samples, including the following methods.
  • Chromatography: Chromatography technology is used to separate and purify marine glycoconjugates and perform component analysis, such as HPLC, capillary electrophoresis, etc.
  • Mass spectrometry (MS): MS provides molecular weight, composition, and sequence information of marine glycoconjugates and helps determine the structure and modification of sugar chains, such as electrospray MS (ESI-MS) and time-of-flight MS (TOF-MS). In addition, chromatography-MS technology is also used to analyze the composition and modification of marine glycoconjugates and identify and quantify various sugar units.
• Structure characterization service: We provide characterization for the three-dimensional structure of marine glycoconjugates, including X-ray crystallography, electron microscopy, and nuclear magnetic resonance (NMR). X-ray crystallography determines the high-resolution three-dimensional structure of marine glycoconjugates, and NMR provides information about the stereochemical characteristics and intermolecular interactions of glycoconjugates.
• Bioactivity assessment service: We use cell experiments and mature biological techniques to evaluate the biological activity and functions of marine glycoconjugates. Common biological activity assessment techniques include cell experiments, enzyme activity determination, and animal model experiments.
• Immunology analysis service: We offer the detection and quantification of specific antigens or antibodies in marine glycoconjugates. Common methods include enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining, and flow cytometry.
• Glycomic service: We provide comprehensive marine glycomic analysis services, including glycan cleavage and modification analysis, glycan composition analysis, and glycan sequencing.

Fig.1 Marine glycoconjugate characterization service. (CD BioGlyco)

Publication

Technology: Catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH) and fluorescent lectin binding analysis (FLBA)

Journal: Systematic and Applied Microbiology

IF: 4.064

Published: 2013

Results: The authors combined CARD-FISH and FLBA to identify glycoconjugates of extracellular polymeric substances (EPS) of Bacteroidetes and diatoms. Complexes were revealed by lectin recognition. At the same time, the authors used confocal laser scanning microscopy to visualize cells within the EPS glycoconjugate matrix. The results showed that only a limited number of lectins throughout the test showed glycoconjugate signals for the sample, and FLBA could reveal the unique spatial distribution of glycoconjugates for a specific Bacteroidetes clade. The combination of CARD-FISH and FLBA held great promise for understanding in situ habitats and substrates of attached viable bacteria.

Fig.2 Visualization of EPS glycoconjugates. (Bennke, et al., 2013)

Applications

• Drug screening: Marine glycoconjugates serve as potential targets or vehicles in drug development. Characterization of marine glycoconjugates reveals their disease-related biological activities, structure-activity relationships, and interaction mechanisms, thereby aiding drug design and optimization.
• Anti-cancer research: Certain marine glycoconjugates have been found to have anti-tumor activity. Characterizing the glycosyl composition, structure, and biological functions of marine glycoconjugates will help reveal their role in the anti-cancer mechanism and provide clues for the development of anti-cancer drugs.
• Biomaterials research: Marine glycoconjugates are often used for the construction and modification of biomaterials. By characterizing the properties of these glycoconjugates and understanding their compatibility, stability, and functionality in biomaterials, we guide the development of new marine-derived biomaterials.

Advantages

• Advanced technology: We have advanced technology for the separation, purification, Profiling, and characterization of marine glycoconjugates to ensure the accuracy and reliability of results.
• Comprehensive analysis methods: We use a variety of comprehensive analysis methods, such as MS, NMR technology, chromatographic separation, etc., to comprehensively characterize the composition, structure, and functions of marine glycoconjugates.
• High-throughput analysis: We have established a convenient high-throughput analysis solution that processes a large number of marine glycoconjugate samples at the same time, speeds up data acquisition, and improves efficiency.

CD BioGlyco masters a variety of detection technologies to offer comprehensive qualitative and quantitative analysis of marine glycoconjugates. We are working on providing high-level marine glycoconjugate and Marine Carbohydrate Characterization services. Please feel free to contact us if you would like to obtain detailed characterization details.