Tailored Floridoside Production Solutions to Meet Specific Client Needs
At CD BioGlyco, our floridoside production service based on our Marine Glycoside Production uses the red microalgae Galdieria sulphuraria to optimize floridoside production under different salt concentrations and carbon source conditions. First, cells are cultured in the salt-free medium until the end of the logarithmic growth phase, and then osmotic stress is applied, which significantly increases floridoside production. The production method consisted of freeze-drying the osmotically stressed cells, extracting the low molecular weight compounds with 80% ethanol, phase separation, and purification of floridoside by thin-layer chromatography (TLC). Ultimately, the identity and purity of floridoside are confirmed by nuclear magnetic resonance (1H-NMR), and high pH anion exchange chromatography (HPAEC-PAD) is used for quantitative analysis. These optimized production and purification methods ensure efficient floridoside production and accurate quality control. Additionally, we also provide other comprehensive Marine Biomolecule Production Services for our clients to help their research.
Fig.1 1H-NMR spectrum of purified floridoside from G. sulphuraria. (Martinez-Garcia & van der Maarel, 2016)
Our floridoside production service uses advanced methods to optimize the production, purification, and characterization of floridoside from G. sulphuraria. Here are the details of the process.
Cultivation
G. sulphuraria. cells are grown heterotrophically in a controlled environment. The cells are cultivated in complete darkness at 40°C with a pH of 2, supplemented with varying concentrations of NaCl (0.5, 1, or 1.5 M).
Osmotic Stress
Osmotic stress is applied by adding NaCl after the cells have reached the late exponential growth phase in a salt-free medium. This strategy significantly enhances the production efficiency and biomass yield.
Extraction
Low-molecular-weight (LMW) compounds are extracted using 80% ethanol through multiple rounds of stirring and ultrasonic bath treatments.
Phase Separation
The extract undergoes phase separation by mixing with ultra-pure water and chloroform, followed by centrifugation. The hydroalcoholic phase is treated with ionic resin to remove impurities, then concentrated under vacuum and freeze-dried.
Purification
The LMW fraction is further purified using preparative TLC on silica gel plates with isopropanol:ethylacetate phase.
Identification and quantification
We use 1H-NMR analysis to confirm the identity and purity of floridoside and use HPAEC-PAD to quantify floridoside.
Publication
Technology: Bioinformatics analysis, qRT-PCR validation, Fluorescence parameter measurement, Iodine staining, Various metabolite content assays
Journal: Marine drugs
IF: 4.9
Published: 2021
Results: The study explored the metabolic pathways of floridean starch and floridoside in the red alga Neoporphyra haitanensis under continuous dark conditions. Through gene identification and comparative metabolite and transcriptomic analyses, it was found that in continuous darkness, the genes responsible for the synthesis of floridean starch were significantly upregulated, while the starch content remained stable. Conversely, the genes involved in the degradation of floridoside were significantly upregulated, leading to a marked decrease in floridoside content.
Fig.2 Floridean starch and floridoside metabolic pathways. (Yu, et al., 2021)
Applications
- Floridoside is valued for its moisturizing properties and ability to protect skin from oxidative stress. It can be used in creams, lotions, and serums to enhance skin hydration and barrier function.
- Floridoside exhibits anti-inflammatory and antioxidative properties, making it a potential ingredient in therapeutic formulations for treating inflammatory skin conditions and promoting wound healing.
- With its prebiotic characteristics, floridoside can be incorporated into dietary supplements to support gut health by promoting the growth of beneficial gut bacteria.
Advantages
- Our production process ensures that floridoside is extracted and purified to high standards, guaranteeing consistent quality for various applications.
- By employing specific cultivation and osmotic stress conditions, our production process maximizes floridoside yield, making the process cost-effective and scalable.
- CD BioGlyco offers tailored production services to meet specific client requirements, allowing for adjustments in the production process to suit different industrial applications.
Frequently Asked Questions
What is Floridoside, and why is it important?
Floridoside is a natural galactosyl glycerol found in red algae. It has significant applications in cosmetics, pharmaceuticals, nutraceuticals, and agriculture due to its moisturizing, anti-inflammatory, antioxidative, and prebiotic properties.
What methods are used for purifying floridoside at CD BioGlyco?
Floridoside is purified using a combination of ethanol extraction, phase separation, and preparative TLC. The final product is verified for identity and purity through 1H-NMR analysis and quantified using HPAEC-PAD.
How can I inquire about CD BioGlyco's floridoside production service?
You can inquire about the service by visiting CD BioGlyco's website and filling out the online inquiry form. Provide details about your project and contact information, and their team will get back to you with more information.
At CD BioGlyco, our floridoside production service offers a reliable and efficient solution for industries seeking high-quality floridoside. Please feel free to contact us for more information if you are interested in our services.
References
- Martinez-Garcia, M.; van der Maarel, M. Floridoside production by the red microalga Galdieria sulphuraria under different conditions of growth and osmotic stress. AMB Express. 2016, 6(1): 71.
- From Wikipedia: https://en.wikipedia.org/wiki/Osmotic_shock.
- From Wikipedia: https://en.wikipedia.org/wiki/Phase_separation.
- Yu, Y.; et al. Floridean starch and floridoside metabolic pathways of and their regulatory mechanism under continuous darkness. Marine drugs. 2021, 19(12).
