The ocean is home to a wide variety of organisms, from single-cell bacteria to large multicellular mammals. The metabolites they produce have good biological activities in anti-cancer, analgesia, antiviral, etc., and are a promising source of new biomedical active molecules. CD BioGlyco is committed to the research of Marine Biomolecules and provides clients with worry-free one-stop services, including extraction, Purification, Characterization, and corresponding application development services of marine biomolecules.
At CD BioGlyco, we help our clients extract Marine Carbohydrates from marine biomolecules using a variety of techniques such as solvent extraction, enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), etc. We use these techniques to help clients release more carbohydrates from complex marine environments. Depending on the client's specific molecules, we choose a specific technique or combine several techniques to ensure extraction rate and purity.
Marine Carbohydrate Production by Organic Solvent Extraction
Organic solvent extraction is the most common and most used polysaccharide extraction technology. We apply this technology to help our clients release more new carbohydrates from the ocean.
Marine Carbohydrate Production by Enzymatic Extraction
Enzyme extraction technology uses specific enzymes to break down cell walls to release carbohydrates. This method is usually carried out under mild conditions and is highly selective and environmentally friendly.
Marine Carbohydrate Production by Assisted Extraction
We use ultrasound, microwaves, etc. to assist in enhancing traditional extraction processes, helping clients increase the yield and purity of carbohydrates and shorten extraction time.
Marine Carbohydrate Production by Supercritical Fluid Extraction
We have accumulated rich experience in supercritical fluid extraction and effectively control the effects of temperature, flow rate, pressure, and other conditions on the total extraction rate.
Marine Carbohydrate Production by Microbes
Microbial extraction uses microorganisms to produce or extract polysaccharides from the ocean. The polysaccharides produced by this method are highly specific and sustainable.
Marine Carbohydrate Production by Precursor Modification
We chemically or enzymatically modify the precursor molecules provided by clients to produce the desired carbohydrates. This method is suitable for producing customized polysaccharides with specific functions.
After extraction, the crude extract usually needs to be purified to remove impurities such as proteins, salts, and other organic compounds. We select the most appropriate purification method according to the characteristics of the crude extract to improve the purity of the polysaccharide and maintain a high yield. Our commonly used purification methods include chromatography purification, precipitation purification, zone electrophoresis purification, salting out purification, metal coordination purification, ultracentrifugation purification, membrane filtration purification, etc.
Characterization of purified molecules helps determine the structural, chemical, and functional properties of marine carbohydrates. Our characterization services include molecular weight determination, chemical composition analysis, functional property analysis, and structural analysis.
Technology: Water extraction, Alkaline solution extraction, Enzyme extraction
Journal: Food Science & Nutrition
IF: 3.55
Published: 2018
Results: The authors used water, alkaline solution, and enzymes to extract crude polysaccharides from Crassostrea rivularis. The results showed that the alkaline solution (KOH) had the highest extraction rate (90.14%) and was the best extraction method. Meanwhile, the authors also explored the effects of extraction temperature and extraction time on the extraction rate of polysaccharides. The results showed that the optimal extraction conditions were KOH concentration of 30% (w/v), temperature of 90°C, and time of 120 min. Under these conditions, the polysaccharide extraction rate was 92.36±0.13%. Then, the authors conducted homogeneity analysis, molecular weight determination, monosaccharide composition analysis, methylation analysis, etc. to characterize the structure of polysaccharides from C. rivularis.
Fig.1 Relationship between KOH concentration (a), temperature (b), and time (c) and extraction rate of glycogen from C. rivularis. (Qin, et al., 2018)
From which marine organisms do we extract marine carbohydrates using these techniques?
What techniques are used to characterize extracted marine biomolecules?
We have a variety of techniques applied to the characterization of marine carbohydrates, such as high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), mass spectrometry (MS), and Fourier transform infrared spectroscopy (FTIR), X-ray diffraction, etc.
CD BioGlyco has accumulated extensive experience in the production of marine carbohydrates. We are constantly optimizing our extraction technology to improve extraction efficiency and product purity. If you are interested in our services, please feel free to
. We hope to help you release more marine carbohydrates.Reference