Marine glycoenzyme detection involves identifying enzymes from marine organisms that are involved in the synthesis, breakdown, or modification of glycans (complex sugar molecules). Detection of these enzymes is typically performed following the screening step of Directed Evolution Experiments. CD BioGlyco is committed to providing a full range of Marine Glycoenzyme Development Services, of which marine glycoenzyme detection service is the last part and is designed to accurately and efficiently detect selected marine glycoenzymes. We provide a variety of technologies for marine glycoenzyme detection, which often rely on labeling strategies to detect and identify glycoenzymes. We offer technologies involving the use of fluorescent labeling, affinity glycan labeling, chemoselective reactions, metabolic glycan labeling (MGL), or chemoenzymatic glycan labeling (CeGL). We also offer mass spectrometry-based analysis for more detailed structural studies.
Fig.1 Marine glycoenzyme detection service. (CD BioGlyco)
- Fluorescent-based Marine Glycoenzyme Detection
This technique utilizes the properties of fluorescence to detect and quantify the presence of glycoenzymes. After glycoenzymes bind to a specific fluorescent marker, they can be detected under certain wavelengths of light. The intensity of the fluorescent signal indicates the amount of glycoenzyme present.
- Affinity Glycan Labeling-based Marine Glycoenzyme Detection
This method uses a "tagged" molecule with a high affinity for the glycoenzyme to be detected. After the molecule binds to the glycoenzyme, a label on the molecule can be detected, showing the presence of the glycoenzyme.
- Chemoselective Reaction-based Marine Glycoenzyme Detection
This type of detection is based on chemical reactions that occur selectively with certain glycoenzymes. The detection is facilitated by the specific products formed from the reactions, indicating the presence of the glycoenzyme.
- MGL-based Marine Glycoenzyme Detection
This technique involves incorporating a labeled monosaccharide into cellular glycans through metabolic processes. The labeled glycans are then detected and quantified.
- CeGL-based Marine Glycoenzyme Detection
This method combines the specificity of enzymes with the sensitivity of chemical detection methods. It involves using enzymes to selectively modify glycans with functional groups, followed by chemically labeling the modified glycans.
- Mass Spectrometry-based Marine Glycoenzyme Detection
This powerful technique uses the principle of ionization and detection of charged particles to identify and quantify the presence of glycoenzymes. Glycoenzymes are ionized and then separated based on their mass-to-charge ratio, allowing for their detection and identification.
Fluorescent-based Marine Glycoenzyme Detection Service
CD BioGlyco provides accurate fluorescent-based marine glycoenzyme detection services to help clients gain an in-depth understanding of the activity of marine glycoenzymes.
Affinity Glycan Labeling-based Marine Glycoenzyme Detection Service
CD BioGlyco provides affinity glycan labeling-based marine glycoenzyme detection services, which accurately detect glycoenzymes through affinity labeling technology to ensure the reliability of experimental results.
Chemoselective Reaction-based Marine Glycoenzyme Detection Service
CD BioGlyco provides chemoselective reaction-based marine glycoenzyme detection services, which use a chemical selection of reaction substrates to provide clients with highly sensitive detection.
MGL-based Marine Glycoenzyme Detection Service
CD BioGlyco provides MGL-based marine glycoenzyme detection services to provide clients with the comprehensive observation of intracellular sugar chain changes.
CeGL-based Marine Glycoenzyme Detection Service
Combining the advantages of chemical and enzymatic reactions, CD BioGlyco provides CeGL-based marine glycoenzyme detection services to ensure highly specific detection of glycoenzyme activity.
Mass Spectrometry-based Marine Glycoenzyme Detection Service
CD BioGlyco provides mass spectrometry-based marine glycoenzyme detection services to help clients gain an in-depth understanding of the characteristics and functions of marine glycoenzymes.
Publication
Technology: High-throughput screening of glycosyltransferases
Journal: Journal of Biological Chemistry
IF: 5.486
Published: 2023
Results: The authors developed an efficient strategy for screening natural product glycosyltransferases in a high-throughput manner. Their method was based on the quick isolation and detection of glycosylated natural products that were chromophoric or fluorescent. The process involved a solvent extraction step that isolated the polar glycoside product from the unreacted aglycone acceptor substrate, followed by the detection of the produced glycoside via the aglycone moiety's inherent absorbance or fluorescence. In this study, they screened a compilation of natural product glycosyltransferases against a selection of precursors critical for therapeutic molecules. Notably, three glycosyltransferases exhibited unexpected promiscuity towards anthraquinones, resulting in the novel manufacture of ε-rhodomycinone glycosides.
Here are some of the results shown in this article:
Fig.2 Screening combinatorial glycosylation of phenolic natural products using the BiG HiT strategy. (Mohideen & Kwan, 2023)
At CD BioGlyco, we have a professional technical team to provide clients with personalized solutions to meet different needs. Please feel free to contact us if you are interested in marine glycoenzyme detection service.
References
- Techner, J.M.; et al. High-throughput synthesis and analysis of intact glycoproteins using SAMDI-MS. Analytical chemistry. 2019, 92(2): 1963-1971.
- Mohideen, F.I.; Kwan, D.H. A "biphasic glycosyltransferase high-throughput screen" identifies novel anthraquinone glycosides in the diversification of phenolic natural products. Journal of Biological Chemistry. 2023, 299(3).
