Antiviral Activity Screening Service

Antiviral Activity Screening Service at CD BioGlyco

Marine glycans have the structural characteristics of acidic groups such as sulfate and glyoxylate, and their interaction with functional proteins on the surface of viruses interferes with the adsorption of viruses to host cells, achieving antiviral effects. CD BioGlyco, with its professional research team, has conducted comprehensive research on the antiviral effects of marine glycans and glycoproteins and proposed a variety of assay and analysis services.

We provide cell activity assay to detect the antiviral activity of marine glycans and glycoproteins. Through the marine glycans and glycoproteins, viruses and live cells co-culture, cell activity detection and analysis, cell characterization observation and comparison, and other operational procedures, to provide clients with accurate marine glycans and glycoproteins antiviral detection activity testing services.

In addition to this, we also offer assaying services that include but are not limited to:

• Infectivity Assay
  The infectivity assay service focuses on analyzing the number and other characteristics of viruses using their infectious effects on host cells.
  We add marine glycans and glycoproteins to a suspension of the virus and cells to perform virus infection experiments. Then, we analyze the antiviral ability of the marine glycans and glycoproteins by examining the infection ability of the virus on the cells before and after the addition of the marine glycans and glycoproteins.
  The methods we have for detecting viruses in this service include but are not limited to, the following.
  • Plaque Assay
  • Endpoint Dilution Assay
    • 50% Tissue culture infective dose (TCID50) assay
    • lethal dose (LD50) assay
    • Egg infective dose (EID50) assay
  • Focus Forming Assay
    The plaque assay and focus formation assay utilizes uninfected cells to calculate the number of viruses. The terminal dilution assay calculates the virus concentration based on the number of dead cells.
• Virus Quantitation Assay
  We offer several methods for viral quantification of the antiviral activity of marine glycans and glycoproteins. Our offered testing methods include but are not limited to:
  • Direct Visualization Based on Electromagnetic (EM) Assay
  • Hemagglutination (HA) Assay
  • Genome Quantification Based on Polymerase Chain Reaction (PCR) Assay
  • Serological Assay
    • Enzyme-linked Immunosorbent Assay
    • Fluorescent-tagged Antibody Assay
    • Precipitation Assay
  • Bicinchoninic Acid Assay
  • Single Radial Immunodiffusion Assay
  • Tunable Resistive Pulse Sensing (TRPS) Assay
  • Flow Cytometry Assay

On the other hand, the antiviral activity of marine glycans and glycoproteins changes significantly after they are modified. Therefore, we also offer an integrated Modification and antiviral assay solution for marine glycans and glycoproteins.

Fig.1 Antiviral activity screening services. (CD BioGlyco)

Publication

Technology: Monolithic adsorption filtration (MAF) and quantitative polymerase reaction (qPCR)

Journal: Journal of Virological Methods

IF: 3.1

Published: 2021

Results: The authors established a workflow for the enrichment of human adenovirus (HAdV) based on MAF and qPCR. In this, targeted synthetic DNA fragments were used as standards for qPCR quantification of HAdV. The use of negatively charged MAF with hydroxylated whole material (MAF-OH) showed better reproducibility and significantly faster turnaround time. The positively charged MAF was then validated using a whole material with a diethylaminoethyl group (MAF-DEAE) for direct concentration of HAdV5 without the need to pre-treat water samples using tap water as a spiking matrix, with less water chemistry definition and control. Finally, using river water as a representative matrix with undefined water chemistry, the authors evaluated the ability of MAF-DEAE to directly enrich HAdV5 from surface water. Meanwhile, MAF-DEAE enables a reproducible HAdV5 recovery independent of the spiked concentration level or sample volume. Furthermore, the authors found that MAF-DEAE reduced the limit of detection of HAdV5 by 115-fold. Hence, this paper describes a workflow derived from MAF-DEAE and qPCR as a facile and efficient alternative to current methods for the rapid detection of HAdV in water.

Fig.2 MAF and qPCR integrated workflow. (Hess, et al., 2021)

Applications

• Marine glycans and glycoproteins antiviral activity assays can be used for the quantitative detection of viruses.
• Marine glycans and glycoproteins antiviral activity assays can be used to develop antiviral drugs made from marine glycans and glycoproteins.
• The marine glycans and glycoproteins antiviral activity assay can be used to investigate the infection mechanism of viruses.

Advantages

• We are committed to providing innovative, efficient, and viable solutions to ensure that our bioactivity assay technology delivers the desired results and creates maximum value for our clients.
• With our advanced technology and high-precision testing capabilities, we offer the ability to analyze and test a wide range of bioactivities in a variety of products.
• We have a team of skilled technicians who are good at solving problems. They have a wealth of expertise and experience in providing custom bioactivity testing solutions to our clients.

CD BioGlyco offers a full range of custom Bioactivity Analysis Services for Marine Glycans and Glycoproteins based on client requirements. Our research team helps you design an analysis protocol based on your needs and interprets the results for the final analysis. If you are interested in our services, please feel free to contact us for more details.