Unlock the Power of Marine Antioxidants with High-Purity D-Isofloridoside from CD BioGlyco
D-isofloridoside (DIF) is a polysaccharide precursor derived from the marine red alga Laurencia undulata, recognized for its potent antioxidant properties and potential health benefits. DIF plays a crucial role in reducing oxidative stress and apoptosis, particularly in liver cells, making it a promising candidate for therapeutic and functional food applications. At CD BioGlyco, our DIF production service is meticulously designed to ensure the efficient extraction, purification, and characterization of high-purity DIF. This production service is achieved by the production of Laurencia undulata in optimal growth conditions, together with moderate osmotic stress to maximize DIF synthesis. DIF is isolated from midguts post-harvest with extraction in 80% ethanol, phase separation (using ultra-pure water and chloroform), followed by preparative thin-layer chromatography (TLC) purification. DIF identity and purity are verified by 1H-NMR spectroscopy with the D- sign on the first carbon of terminal diaminoamide, quantified using high pH anion exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD). Moreover, we also offer serval Marine Biomolecule Production Services for clients to speed up their research.
Fig.1 Extraction and purification of DIF. (Yang, et al., 2020)
CD BioGlyco offers a comprehensive production service for the high-purity DIF, which ensures efficient extraction, purification, and characterization. Here are the details of the DIF production process.
- Laurencia undulata is cultivated under controlled conditions to maximize the production of DIF. The algae are grown in a nutrient-rich medium with specific parameters.
- After the algal biomass is harvested, it undergoes a series of extraction steps to isolate DIF.
- Ethanol extraction: The dried algal biomass is treated with 80% ethanol.
- Phase separation: The ethanol extract is mixed with ultra-pure water and chloroform to separate the different phases.
- The extracted compounds are subjected to purification to isolate DIF.
- Ion exchange treatment: The hydroalcoholic phase is treated with Amberlite MB20 resin to remove impurities.
- Concentration and drying: The treated solution is concentrated under vacuum and then freeze-dried to obtain the crude DIF extract.
- TLC: The crude extract undergoes preparative TLC on silica gel plates, this step ensures the separation of DIF from other compounds.
- The purified DIF is then characterized to confirm its identity and purity.
- 1H-NMR spectroscopy: NMR spectroscopy is used to verify the molecular structure of DIF.
- Quantification: HPAEC-PAD is employed to accurately quantify the amount of DIF.
Publication
Technology: Cell viability assays, Reactive oxygen species (ROS) measurement, Western blot analysis, Comet assays for DNA damage, Molecular docking
Journal: Molecules (Basel, Switzerland)
IF: 4.2
Published: 2020
Results: The study investigates the protective effects of DIF, a polysaccharide precursor from the marine red alga Laurencia undulata, on alcohol-induced hepatotoxicity in HepG2 cells. The research demonstrates that DIF significantly mitigates alcohol-induced cytotoxicity, reduces intracellular ROS, and minimizes DNA damage in liver cells. DIF was found to upregulate antioxidant proteins like glutathione (GSH) and superoxide dismutase (SOD), while downregulating proteins associated with oxidative stress and apoptosis such as γ-glutamyl transferase (GGT), Bax, cleaved caspase-3, and mitogen-activated protein kinase (MAPK) signaling proteins.
Applications
- DIF has shown significant potential in protecting liver cells from alcohol-induced oxidative damage. This makes it a promising candidate for developing drugs and therapeutic formulations aimed at preventing and treating liver diseases.
- In the agricultural sector, DIF can be used as a biostimulant to improve plant growth and stress tolerance, enhancing crop yield and resilience against environmental stressors.
Advantages
- CD BioGlyco employs advanced purification techniques to ensure that the DIF produced is of high purity and quality.
- We use sophisticated analytical methods such as 1H-NMR spectroscopy for structural confirmation and HPAEC-PAD for quantification to ensure precise identification and measurement of DIF.
Frequently Asked Questions
What applications can benefit from CD BioGlyco's DIF?
DIF can be added to foods to enhance their antioxidant capacity and health benefits. And it can be used in formulations to protect against liver damage and oxidative stress.
Why choose CD BioGlyco for DIF production?
CD BioGlyco ensures high-quality production of DIF through optimized cultivation and extraction processes, advanced purification techniques, and rigorous characterization methods. Their service provides customized production solutions to meet specific client needs, ensuring consistent quality and efficacy of DIF for various applications.
At CD BioGlyco, based on our advanced biotechnology we provide the DIF production service that stands out as a reliable and efficient solution for producing this valuable marine-derived compound. Please feel free to contact us for more information if you are interested in our Marine Glycoside Production Services.
References
- Yang, S.; et al. The protective effect of the polysaccharide precursor, D-isofloridoside, from on alcohol-induced hepatotoxicity in HepG2 cells. Molecules (Basel, Switzerland). 2020, 25(5).
- From Wikipedia: https://en.wikipedia.org/wiki/Laurencia#/media/File:Laurencia_microcladia_(herbarium_item).jpg.
- Clos, L.J.; et al. NMRbot: Python scripts enable high-throughput data collection on current Bruker BioSpin NMR spectrometers. Metabolomics: Official journal of the Metabolomic Society. 2013, 9(3).
