Precision Quantification for Deeper Marine Insights – CD BioGlyco's Advanced Proteomics Solutions
CD BioGlyco offers comprehensive services for the absolute quantification analysis of marine glycoproteins, utilizing advanced targeted quantitative proteomics techniques. These services are pivotal for accurately determining the structural and functional roles of glycoproteins in marine carbohydrates. By employing multiple reaction monitoring (MRM) or selective reaction monitoring (SRM), CD BioGlyco ensures precise and reliable quantification of proteins and their modification states. This level of accuracy is essential for a wide range of research applications, including environmental monitoring, biotechnology, and pharmaceutical development. The ability to measure the absolute content of glycoproteins allows researchers to gain deeper insights into marine microbial dynamics, facilitating the development of innovative marine-derived products.
Sample Collection and Preparation
Marine samples are collected from the target environment and then filtered or centrifuged to concentrate microbial cells or glycoproteins. Following concentration, the cells or tissues are lysed to release proteins, which are subsequently extracted and purified using specialized kits optimized for marine samples.
Protein Digestion
The extracted proteins are enzymatically digested into peptides using specific proteases, such as trypsin, to facilitate downstream analysis.
Peptide Labeling (optional)
Peptides may be labeled with stable isotopes to enhance quantification accuracy during mass spectrometry analysis.
Separation and Quantification
Peptides are separated using liquid chromatography to reduce sample complexity and improve detection sensitivity. Following separation, the peptides are analyzed using MRM or SRM mass spectrometry to determine the absolute content of target glycoproteins.
Data Analysis
The absolute abundance of glycoproteins is quantified based on the intensity of the detected peptide signals, with data normalization using internal standards or spiked-in reference markers to account for variations in sample processing and instrument performance.
Reporting
Results are interpreted in the context of the study objectives, providing insights into the structural and functional roles of marine glycoproteins. A comprehensive report detailing the methods, results, and conclusions of the analysis is generated and delivered to the client.
Follow-up Services
CD BioGlyco offers follow-up consultations to discuss the findings and their implications, as well as to plan further research if needed. Clients may also opt for additional services such as Marine Glycomics Services or Marine Glycoprotein Production Services to further explore their research interests.
Publication
DOI: 10.3390/microorganisms9091797
Technology: Spike-In, Flow cytometry, 16S qPCR, qRT-PCR
Journal: Microorganisms
IF: 4.1
Published: 2021
Results: This article discusses several advanced methods for the absolute quantification of marine glycoproteins and other microbial taxa. These methods include the Spike-In method with internal microorganism markers, flow cytometry for enumerating bacterial groups based on physical and chemical characteristics, 16S quantitative real-time polymerase chain reaction (qPCR) and qRT-PCR techniques for detecting and quantifying bacterial genes, density gradient centrifugation for separating substances based on size and density, and the highly sensitive catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH) technique. These methods are crucial for accurate microbial community analysis, especially in marine environments, where understanding the absolute quantification of bacteria can provide insights into ecological dynamics and interactions.
Frequently Asked Questions
Can CD BioGlyco provide follow-up consultations after the analysis?
Yes, CD BioGlyco offers follow-up consultations to discuss the findings, and their implications, and to plan further research if needed. We also provide additional services such as Marine Carbohydrate Modification Service, Bioactivity Analysis of Marine Glycan&Glycoprotein, and Marine Glycoenzyme Development Service.
What technologies are used for the separation and quantification of peptides?
We use liquid chromatography (LC) to separate peptides, reducing sample complexity and enhancing detection sensitivity. Following separation, peptides are quantified using MRM or SRM mass spectrometry.
How are proteins digested in the analysis process?
Extracted proteins are enzymatically digested into peptides using specific proteases like trypsin. This step is crucial for breaking down the proteins into smaller peptides suitable for downstream mass spectrometry analysis.
Applications
- Marine glycoproteins are potential sources of new pharmaceuticals. Absolute quantification helps identify promising candidates by accurately measuring their abundance in marine organisms.
- Quantification techniques are used to optimize the production of glycoproteins for various industrial applications, including bioremediation and biofuel production.
- Glycoproteins from marine organisms can be studied for their potential role in human health, including their antimicrobial and anti-inflammatory properties.
Advantages
- CD BioGlyco employs advanced techniques, ensuring highly precise and accurate quantification of glycoproteins and their modification states.
- Our services cater to a wide range of marine samples, including water, sediment, and biological specimens.
- Clients receive a thorough report detailing the methods, results, and conclusions of the analysis.
At CD BioGlyco, our absolute quantification analysis of marine glycoproteins employs advanced techniques, providing precise and reliable data crucial for various research applications. Please do not hesitate to contact us for more details about our absolute quantification analysis service.
Reference
- Wang, X.; et al. Current applications of absolute bacterial quantification in microbiome studies and decision-making regarding different biological questions. Microorganisms. 2021, 9(9): 1797.
