Dihydroxypropyl-chitosan Adjuvant Development Service

Dihydroxypropyl-chitosan Adjuvant Development Service at CD BioGlyco

CD BioGlyco has accumulated extensive expertise in the development of Marine Carbohydrate-based Vaccine Development and Marine Carbohydrate-based Adjuvant Development. With a strong focus on harnessing the potential of natural resources, our research and development team has dedicated years to exploring the unique properties and applications of marine carbohydrates. Our advanced laboratory ensures the ability to develop various kinds of dihydroxypropyl-chitosan marine carbohydrate-based adjuvants.  

• Slow-release Adjuvant Development
  We prepare dihydroxypropyl chitosan in the form of microspheres, nanoparticles, etc., along with the drug as a sustained-release adjuvant. We hope to contribute to your research in controlling the rate and time of drug release, prolonging the duration of drug action in the body, and improving the bioavailability and therapeutic efficacy of drugs.
• Targeted Adjuvant Development
  We introduce specific targeting molecules, such as receptors, antibodies, oligonucleotides, etc., on the surface of carriers such as dihydroxypropyl chitosan nanoparticles or liposomes through surface modification technology. Then through chemical reaction, the dihydroxypropyl chitosan is covalently bound to the targeting molecules. This enables the targeting molecule to be tightly bound to the dihydroxypropyl chitosan carrier, improving the stability and targeting performance of the targeting adjuvant. Then we form the composite adjuvant by alternately stacking dihydroxypropyl chitosan and targeting molecules on the carrier surface through layer-by-layer assembly technology. Finally, we co-mixing dihydroxypropyl chitosan with targeting molecules and other auxiliary materials.
• Optical Imaging Adjuvant Development
  By mixing a fluorescent dye into dihydroxypropyl chitosan nanoparticles, hydrogels, or coatings. This fluorescent dye absorbs energy from an external light source and emits fluorescence at a specific wavelength, enabling optical imaging. Commonly used fluorescent dyes include oxalins, thiomers, and quantum dots.
• Other Adjuvant Development

Fig.1 Technologies used in the development of dihydroxypropyl chitosan adjuvant. (CD BioGlyco)

Publication Data

Technology: Nanoparticle preparation, drug encapsulation technology, and drug loading and release testing

Journal: Carbohydrate polymers

IF: 10.723

Published: 2013

Results: The main focus of this article is on the preparation of nanocolloid micelles using a new amphiphilic chitosan derivative, N-(2,3-dihydroxypropyl)–chitosan–cholic acid (DHP-CS-CHO), and the loading of the anticancer drug paclitaxel (PTX) into the nanocolloid micelles. In the article, chemical modification of chitosan was used to obtain the amphiphilic chitosan derivative DHP-CS-CHO by reacting cholic acid and propylene oxide with the pristine amine group of chitosan, respectively. Then, the DHP-CS-CHO nanocellulose was prepared by self-assembly and PTX was loaded into the nanocellulose by dialysis. The article also investigated the release behavior of PTX from the nanocolloids and the antitumor effect of PTX-loaded nanocolloids. The PTX-loaded DHP-CS-CHO micelles not only showed sustained release of PTX but also demonstrated in vitro experiments that these micelles were able to inhibit the growth and induce apoptosis of MCF-7 cells. Therefore, DHP-CS-CHO may be a promising carrier for the anticancer drug PTX.

Fig.2 Chemical structure of Cy5.5 labeled and Gd(III) encapsulated chitosan nanoparticles and schematic illustration of self-assembled chitosan nanoparticles. (Pan, et al., 2013)

Applications

• Dihydroxypropyl chitosan is used as a carrier material in drug delivery systems for encapsulating and releasing anticancer drugs, anti-infective drugs, and other drugs.
• Dihydroxypropyl chitosan has good biocompatibility and biodegradability and is used in the preparation of biodegradable medical materials.
• Due to its cationic nature, dihydroxypropyl chitosan is used in the preparation of antimicrobial materials, such as antimicrobial coatings, which have certain antimicrobial effects.

Highlight

• Dihydroxypropyl chitosan has tunable physicochemical properties and can be functionally modified and structurally optimized in a variety of ways to achieve different application needs.
• Dihydroxypropyl chitosan sustained release adjuvant allows the drug to maintain a stable concentration in the body for a longer period, reducing the frequency of drug use as well as the dosage of the drug, thus improving the drug effect and reducing the side effects of the drug.
• Dihydroxypropyl chitosan adjuvant can be functionally modified by chemical modification and cross-linking to provide controlled release, enhanced stability, improved drug solubility, and other properties for different types of drugs.

At CD BioGlyco, we combine our marine carbohydrate-based vaccine development service with our multi-technology-based dihydroxypropyl-chitosan adjuvant development service to provide you with a more comprehensive research perspective. If you are interested in us, please feel free to contact us.