Enabling Marine Glycobiology Research Through the Production of High-quality Stanniocalcin
Stanniocalcin is a homodimeric glycoprotein, first discovered in fish, that plays a key role in phosphate and calcium homeostasis. CD BioGlyco offers an innovative stanniocalcin production service based on extensive experience with Marine Biomolecules production such as Marine Glycoproteins and high-quality personnel. The structural integrity and biological activity of stanniocalcin are ensured through an efficient production process.
Production programs may vary for different materials. We assess our client's needs and research objectives in detail and customize stanniocalcin production solutions. The production process includes the extraction, purification, and characterization of stanniocalcin as shown below:
Extraction
This step involves pretreatment of the material (fish) and extraction using appropriate solvents (alkaline ethanol, etc.). We optimize individual factors to ensure the yield, stability, and biological activity of stanniocalcin.
Purification
We obtain stanniocalcin by centrifugation, precipitation, and chromatography (gel filtration chromatography, ion exchange chromatography, or reversed-phase high-performance liquid chromatography) to isolate and purify it from the extracted red.
Characterization
Stanniocalcin is analyzed for purity, structure, molecular weight, and biological activity using sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE), western blot, mass spectrometry, etc.
- Total protein content
- Detection of molecular weight distribution and protein patterns
- Amino acid sequence
- Structural analysis
- Mass spectrometry analysis.
- Determination of biological activity
Publication Data
Technology: Enzyme-linked immunosorbent assay
Journal: PLoS One
IF: 3.054
Published: 2014
Results: The present study compares the differences in stanniocalcin content between male and female Mastacembelus armatus during the gonadal cycle. Stanniocalcin, a hormone involved in calcium regulation, varies in M. armatus levels between sexes during the reproductive cycle. The results showed that stanniocalcin is involved in calcium homeostasis and gonadal maturation in females, but not in calcium homeostasis regulation in males.
Fig.1 Changes such as stanniocalcin in male M. armatus. (Verma & Alim, 2014)
Applications
- Stanniocalcin is found to play a key role in calcium homeostasis. It is used in studies of therapeutic approaches to calcium homeostasis, such as hypercalcemia.
- Stanniocalcin is used to study its effect on calcium and phosphorus levels in animals.
- Stanniocalcin is used as a marker in the study of diseases such as cancer.
Advantages
- We have tested the effect of individual parameters on stanniocalcin yield and optimized the entire production process.
- After an efficient isolation, purification, and characterization process, we provide high-quality stanniocalcin products, comprehensive analytical reports, and research guidance.
- We perform a detailed and comprehensive characterization of the extracted stanniocalcin, including molecular weight analysis, structure, and bioactivity determination.
Frequently Asked Questions
What is the physiological significance of stanniocalcin?
Stanniocalcin is a disulfide-linked homoglycosylated hormone first isolated from small endocrine glands in the kidneys of scleractinian and holothurian fish. Stanniocalcin is found to be a true regulator of calcium entry through the gut and gills. In fish, stanniocalcin acts in the intestine, kidneys, and gills to regulate serum phosphate and calcium levels and is a key endocrine factor.
From which species has stanniocalcin been identified and isolated so far?
Stanniocalcin is a glycosylated peptide hormone that has been shown to play a key role in calcium and phosphate homeostasis. It has been identified and isolated from flounder, rainbow trout, Japanese eel, seawater salmon, etc., and the related genes and physiological functions have been studied.
CD BioGlyco offers a one-stop shop for stanniocalcin production by combining a variety of advanced methods and technologies. We remain at the forefront of marine glycobiology and provide innovative production solutions and research support. Please feel free to contact us for further details on stanniocalcin production, should you be interested or require it.
Reference
- Verma, S.K.; Alim, A. Differential activity of stanniocalcin in male and female fresh water teleost Mastacembelus armatus (Lacepede) during gonadal maturation. PLoS One. 2014, 9(7): e101439.
