CD BioGlyco's Advanced Production Services for High-Purity Aquastatin A!
CD BioGlyco offers specialized production services for aquastatin A, a compound characterized by the molecular formula C36H52O12. This compound features two aromatic rings and a galactose unit, and it appears as a white powder. Aquastatin A is known for its potent inhibition of mammalian adenosine triphosphatases, including Na+/K+-ATPase and H+/K+-ATPase, with IC50 values of 7.1 µM and 6.2 µM, respectively. Aquastatin A is initially isolated from the fungus Fusarium aquaeductuum, which was collected from slime fluxes in Karuizawa, Nagano Prefecture, Japan. CD BioGlyco employs advanced fermentation and extraction techniques to Marine Glycoside Products and purify aquastatin A from this fungal source. Additionally, we offer clients several Marine Biomolecule Production Services according to their needs for helping their research.
The production process involves fermenting Fusarium aquaeductuum in GPMY medium, followed by extraction and isolation. The fermentation is carried out in a 60-liter tank, reaching a maximum titer of 1,367 µg/ml after 168 hours. The culture broth is filtered, and the mycelial part is extracted with acetone and aqueous acetone. The crude extract is then purified using reverse-phase high-performance liquid chromatography (HPLC) to yield aquastatin A as a white powder.
Fermentation
Grow Fusarium aquaeductuum in GPMY medium
Culture in a 60-liter tank at 26°C for 7 days, maintaining dissolved oxygen above 25%.
Filter the culture broth to separate the mycelial part.
Extraction
Extract the mycelial part with acetone and aqueous acetone.
Combine and concentrate the extracts under reduced pressure.
Adjust the water phase to pH 3.0 and extract with ethyl acetate.
Purification
Dissolve the crude extract in methanol.
Apply to a C18 reverse-phase column and elute with 70% acetonitrile and 30% triethylammonium phosphate buffer.
Combine the peak fractions containing aquastatin A and evaporate acetonitrile.
Extract the residual solution with ethyl acetate and evaporate to obtain aquastatin A as a white powder.
Quality Testing
Perform analytical HPLC using a C18 reverse-phase column.
Use nuclear magnetic resonance (NMR) (1H, 13C) and mass spectrometry (MS) such as fast atom bombardment-MS (FAB-MS) and MS-MS for structure determination.
Conduct enzyme assays to measure Na+/K+-ATPase and H+/K+-ATPase inhibition.
Evaluate biological properties, including diuretic, natriuretic, and gastric secretory studies.
Publication
Technology: 1H NMR, MS/MS
Journal: International journal of molecular sciences
IF: 4.9
Published: 2015
Results: Research on aquastatin A, sourced from the marine fungus Cosmospora sp. The compound's structure, confirmed through spectroscopic methods like 1H NMR and MS/MS, features a dihydroxypentadecyl benzoic acid moiety that contributes to its inhibitory properties. This study positions aquastatin A as a promising candidate for the treatment of type 2 diabetes and obesity, owing to its specific inhibition of PTP1B over other protein tyrosine phosphatases, including TCPTP, SHP-2, LAR, and CD45.
Applications
- Aquastatin A's significant inhibitory activity against protein tyrosine phosphatase 1B (PTP1B) makes it a promising candidate for developing new therapies for type 2 diabetes.
- Aquastatin A can be utilized in research to study the role of PTP1B in cellular signaling pathways. This can help uncover new insights into the molecular mechanisms of various diseases and identify new therapeutic targets.
- With its selective inhibition of PTP1B, Aquastatin A serves as an excellent lead compound for developing new drugs aimed at treating metabolic disorders.
Advantages
- CD BioGlyco uses sophisticated purification methods, including reverse-phase HPLC to ensure aquastatin A is isolated in its purest form.
- The production process is supported by cutting-edge analytical techniques such as NMR and MS (FAB-MS and MS-MS) to verify the structure and purity of aquastatin A.
- CD BioGlyco tailors the production process to meet specific client requirements, ensuring optimal yield and functionality of aquastatin A for research and industrial purposes.
Frequently Asked Questions
What makes CD BioGlyco's aquastatin A production service unique?
CD BioGlyco's service is distinguished by its use of advanced technologies, high customization to client needs, and rigorous quality control. These factors ensure the production of high-purity aquastatin A with preserved bioactive properties, making it suitable for a wide range of applications.
Can CD BioGlyco customize the production process for specific research needs?
Yes, CD BioGlyco offers custom production services tailored to meet the specific requirements of clients. This includes adjustments in the production process to achieve desired purity levels and quantities for various research and industrial applications.
CD BioGlyco provides high-quality production service for aquastatin A, which supports our clients in helping their research and development goals. Please do not hesitate to contact us for more information if you are interested in our services and technologies.
References
- Clos, L.J.; et al. NMRbot: Python scripts enable high-throughput data collection on current Bruker BioSpin NMR spectrometers. Metabolomics: Official journal of the Metabolomic Society. 2013, 9(3): 558-563.
- Kang, H.; et al. The effects of marine carbohydrates and glycosylated compounds on human health. International journal of molecular sciences. 2015, 16(3): 6018-6056.
- From Wikipedia: https://en.wikipedia.org/wiki/Fusarium#/media/File:Fusarium_chlamydospores_160X.png.
- From Wikipedia: https://en.wikipedia.org/wiki/High-performance_liquid_chromatography#/media/File:Hplc-perfume-chromatogram.png.
