Unlock Antifungal Potential with CD BioGlyco's Marmoratoside Production Services
CD BioGlyco offers comprehensive marmoratoside production services, this service focuses on extracting these bioactive glycosides marmoratoside from the sea cucumber such as Bohadschia marmorata. Marmoratosides, including marmoratoside A and B, are holostan-type triterpenoid glycosides known for their significant antifungal activity against several pathogenic fungi, including Candida albicans and Aspergillus fumigatus. These compounds exhibit a structure characterized by a triterpene aglycone core connected to various glycan units. We also provide other Marine Glycoside Production Services for clients to choose the most suitable solution for their research goals.
Fig.1 Marmoratoside A (24) and marmoratoside B (26). (Kang, et al., 2015)
Our service ensures the bioactivity and purity of marmoratosides, making them valuable for studying their therapeutic potential, particularly in antifungal treatments. Moreover, we also offer comprehensive Marine Biomolecule Production solutions for clients worldwide. Here are the steps of our marmoratoside production service.
Extraction
Bioactive glycosides marmoratosides are obtained from the Chinese sea cucumber Bohadschia marmorata. This is accomplished using a solvent extraction technique, which effectively isolates the crude mixture of compounds.
Fractionation
Following extraction, the crude extract is subjected to fractionation. This step is crucial for enriching the glycoside content and is typically performed using methods such as liquid-liquid extraction or various chromatographic techniques. This process helps in concentrating the marmoratosides from other components.
Purification
The enriched fractions then undergo purification to achieve high purity and enhance bioactivity. CD BioGlyco utilizes advanced chromatographic methods, specifically high-performance liquid chromatography (HPLC), to isolate pure marmoratosides. This step ensures that the final product is free from impurities and has a high concentration of active glycosides.
Characterization and Quality Control
The last stage involves rigorous characterization and quality control to verify the structural integrity and purity of the Marmoratosides. Nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) are employed to confirm the precise structure and composition of the glycosides. These analytical techniques ensure that the marmoratosides produced meet the stringent quality standards required for research and therapeutic applications, guaranteeing their efficacy and reliability.
Publication
DOI: 10.3390/antibiotics9110813
Technology: Solvent extraction, NMR, MS
Journal: Antibiotics
IF: 4.3
Published: 2020
Results: The research on marmoratoside focuses on its significant antifungal activity against Aspergillus fumigatus, as well as its extraction and characterization. Marmoratoside, a triterpene glycoside isolated from the sea cucumber Bohadschia marmorata, demonstrates a potent inhibitory effect on fungal pathogens. The study utilized several key techniques, including solvent extraction, chromatography for fractionation and purification, NMR spectroscopy, and MS for structural verification and quality control.
Applications
- Marmoratosides are potent antifungal agents effective against pathogens such as Aspergillus fumigatus and Candida albicans.
- The unique structure and bioactivity of marmoratosides make them suitable for studying their effects on various biological systems.
- As compounds derived from marine organisms, marmoratosides contribute to the growing field of natural product research, which seeks to discover and develop new drugs from natural sources.
Advantages
- The use of NMR spectroscopy and MS guarantees the structural integrity and purity of the marmoratosides, providing reliable compounds for research.
- Our production process is scalable, catering to both small-scale academic research and larger industrial applications, ensuring that researchers receive the amount of compound they need for their studies.
- Our company works closely with researchers to provide technical support and guidance, helping to maximize the potential of marmoratosides in various applications.
Frequently Asked Questions
How is the extraction and purification of marmoratosides performed?
The extraction process begins with solvent extraction from the sea cucumber tissues, followed by fractionation and purification using chromatographic techniques like HPLC. This ensures the high purity and bioactivity of the marmoratosides.
What applications do marmoratosides have?
Marmoratosides are primarily used in antifungal research due to their potent activity against pathogens like Aspergillus fumigatus. They are also studied for their broader therapeutic potential in treating fungal infections and possibly other diseases.
At CD BioGlyco, our marmoratosides production service provides valuable materials for clients with their cancer research. Please do not hesitate to contact us for more details about our production service and feel free to get in touch for your custom solution.
References
- Kang, H.; et al. The effects of marine carbohydrates and glycosylated compounds on human health. International journal of molecular sciences. 2015, 16(3): 6018-6056.
- Ahamefule, C.S.; et al. Marine bioactive compounds against Aspergillus fumigatus: challenges and future prospects. Antibiotics. 2020, 9(11): 813.
- From Wikipedia: https://en.wikipedia.org/wiki/Bohadschia_marmorata#/media/File:Bohadschia_marmorata.png.
- From Wikipedia: https://en.wikipedia.org/wiki/Liquid%E2%80%93liquid_extraction#/media/File:Funnels.jpg.
- From Wikipedia: https://en.wikipedia.org/wiki/High-performance_liquid_chromatography#/media/File:Agilent1200HPLC.jpg.
