Precise Glycoprotein Quantification at Our Fingertips
CD BioGlyco provides cutting-edge services for the relative quantification of marine glycoproteins, using sophisticated mass spectrometry (MS) techniques. This method is particularly useful for identifying changes in protein expression under various conditions, such as different environmental factors, disease states, or treatment regimens. Besides our relative quantification analysis, we also offer Marine Glycomics Services for clients for a more comprehensive analysis of interested protein. Our Marine Glycoprotein Production Services section is a powerful tool for you to choose the marine glycoprotein you like to use in research.
Sample Requirements
- Sample type: Marine organisms such as algae, seaweeds, marine bacteria, or tissues from marine animals containing glycoproteins.
- Sample preparation: Samples should be fresh or properly preserved (e.g., frozen or lyophilized) to maintain protein integrity. A minimum amount of sample is required to ensure sufficient protein extraction, typically in the milligram range.
The Process of Our Relative Quantification Analysis of Marine Glycoprotein
- Proteins are extracted from the marine samples using appropriate buffers and mechanical disruption methods such as sonication or homogenization.
- Extracted proteins are broken down into peptides using enzymes such as trypsin.
- For isotope labeling techniques such as isotope-coded affinity tags (ICAT) or isobaric tags for relative and absolute quantitation (iTRAQ), peptides are labeled with isotopic tags to facilitate relative quantification.
- Peptides, whether labeled or not, are separated by liquid chromatography (LC) to simplify the sample.
- Separated peptides are analyzed using mass spectrometry, including techniques like Matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF MS) or LC-MS/MS.
Output Results
The relative abundance of glycoproteins in each sample is determined based on the intensity of the mass spectrometry signals. For isotope-labeled samples, the ratio of isotopic tags indicates the relative quantities of peptides from different samples.
Data analysis software processes the mass spectrometry data to compare glycoprotein levels across samples. Quantitative comparisons highlight changes in glycoprotein expression under different conditions or treatments.
The analysis identifies specific glycoproteins present in the samples, providing insights into their roles and functions. Information on glycoprotein structure and modification (e.g., glycosylation sites) is also obtained.
Statistical analysis is used to determine the significance of differences in glycoprotein levels.
Publication
DOI: 10.3389/fmicb.2022.826942
Technology: Relative quantification analysis
Journal: Frontiers in Microbiology
IF: 4
Published: 2022
Results: The document provides a detailed analysis of the relative quantification of marine glycoproteins, specifically focusing on glyceroglycolipids from marine algae. These compounds, abundant in marine algae, have been recognized for their significant pharmacological properties, including anti-inflammatory, anti-viral, anti-cancer, and antibacterial activities. The analysis emphasizes the structural diversity of glyceroglycolipids, which consist of various glycosyl and acyl chains, contributing to their broad biological activities.
Frequently Asked Questions
How long does the analysis process take?
The time required for analysis varies with the number and complexity of samples, typically taking a few weeks from receiving the sample to delivering the final report. An estimated timeline will be provided when the project begins.
What information will I receive from the analysis?
You will receive detailed data on the relative abundance of glycoproteins in your samples, quantitative comparisons highlighting changes in protein expression, identified glycoproteins, and information on glycoprotein structure and modification. A comprehensive report with statistical analysis, visual representations, and key findings will also be provided.
Applications
- This service can be used in investigating how glycoprotein expression varies under different conditions, such as stress, disease, or treatment.
- This service can be used in identifying glycoproteins that serve as biomarkers for specific diseases or conditions.
- This service can be used in understanding the roles of glycoproteins in cellular processes and signaling pathways.
Advantages
- Our advanced MS techniques ensure accurate and sensitive detection of glycoproteins.
- We have the ability to analyze multiple samples simultaneously, providing a thorough understanding of protein expression dynamics.
- Our services cater to a variety of research needs, ranging from fundamental biology to clinical studies.
At CD BioGlyco, our relative quantification analysis service provides researchers with detailed and reliable data on glycoprotein expression, supporting advanced studies in marine biology, medicine, and biotechnology. Please do not hesitate to contact us for more details about our analysis service.
Reference
- Tian, X.; et al. Comparative proteomic analysis of fucosylated glycoproteins produced by Bacteroides thetaiotaomicron under different polysaccharide nutrition conditions. Frontiers in Microbiology. 2022, 13: 826942.
