Tailored Glycan Profiling of Intact Marine Glycoprotein for Advanced Research
Glycosylation is a common post-translational modification of proteins that affects their function and activity. As a leader in the field of glycoprotein analysis, CD BioGlyco has rich experimental experience in Quantitative and Structural Analysis of Marine Glycoproteins. We have established scientific and standardized quality control standards to ensure the reliability and accuracy of results. We provide a professional and customized glycan profiling service for complete marine glycoproteins to identify and characterize glycan types, structures, and relative contents.
Glycan Profiling of Intact Marine Glycoprotein at CD BioGlyco
We have extensive experience in Marine Glycoproteomics Analysis and have developed a simple and efficient glycan profiling process. Combined with advanced analytical techniques, the specific experimental flow is as follows:
- Material pre-processing: We extract intact glycoproteins from different marine sources (plants and animals), including cell lysis, extraction, purification, and concentration of glycoproteins.
- Release of glycans: This process involves the release of glycans from glycoproteins either chemically or enzymatically.
- Derivatization of glycans: We perform fluorescent labeling or other derivatization of glycans to enhance the mass spectrometric response of glycans for identification.
- Glycan analysis: Glycans are analyzed by liquid chromatography-mass spectrometry (LC-MS) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Afterward, the relative contents of different glycan types are quantified according to the corresponding mass spectrometry peak areas, and bioinformatics tools are used to identify the glycan types and obtain the fine structure information of glycan chains.
Applicable Samples
Our glycan profiling service applies to all types of samples. Clients just need to tell us the experiment objectives and requirements. We complete all subsequent experiments.
- Glycoprotein samples
- Cell samples
- Tissues (plant and animal tissues)
- Other types of samples
Results Delivery
We provide a detailed analysis including the following:
- Principles and steps of glycan profiling
- Relevant mass spectrometry parameters
- Raw data
- Mass spectra
- Glycan profiling results
Publication Data
Technology: LC-MS
Journal: Frontiers in Plant Science
IF: 6.627
Published: 2021
Results: This study analyzes in detail the N-glycans and N-glycoproteins in Phaeodactylum tricornutum. The researchers identified 59 glycoproteins corresponding to N-glycans (mixed and high-mannose type N-glycans) through a series of treatments of extraction, digestion, enrichment, and labeling. Among them, the high-mannose-type N-glycans contained four types and all carried terminal glucose residues. This study provides a demonstration of the method for the analysis of N-glycan structures and updates the database of N-glycans in microalgae. It also provides a theoretical basis for elucidating the functions of these N-glycoproteins and for designing the use of microalgae for N-glycoprotein production.
Fig.1 N-glycans of five species identified in P. tricornutum. (Xie, et al., 2021)
Applications
- Glycan profiling plays an essential role in the production and quality control of marine glycoproteins.
- Glycan profiling plays an important role in understanding the glycosylation pathways of marine organisms and designing processes for the production of glycoproteins using marine organisms.
- Glycan profiling helps to discover novel glycan structures and provides useful information for the development of biomaterials, drugs, etc.
Advantages
- We have extensive hands-on experience in handling complex samples and analyzing marine glycoprotein structures.
- We customize glycan profiling protocols based on the sample type and experimental objectives to meet our client's research needs.
- Through professional and comprehensive glycan profiling, we help to accurately interpret complex data and provide valuable insights.
Frequently Asked Questions
Why analyze glycans in marine glycoproteins?
Glycans play an essential role in the structure and activity of glycoproteins. An in-depth analysis of the composition of glycans helps to understand how they regulate the function, activity, interaction with other molecules, cell migration, and adhesion of marine glycoproteins.
Can you process samples?
Of course, we do the complete process from sample pretreatment, glycoprotein extraction, and glycan release to analysis on our side. Depending on the type of sample, the experimental program will be different. Specific treatment methods and analysis procedures will be communicated and confirmed with you before the start of the experiment.
CD BioGlyco customizes glycan profiling protocols for complete marine glycoproteins according to the specific needs of our clients. Our goal is to help unravel the intricate structure and function of these molecules in marine ecosystems. Please feel free to contact us if you need more information about glycan profiling.
Reference
- Xie, X.; et al. Global profiling of N-glycoproteins and N-glycans in the diatom Phaeodactylum tricornutum. Frontiers in Plant Science. 2021, 12: 779307.
