Harnessing Marine's Power: Sustainable Carbohydrates by Marine Microbes
Marine Carbohydrate Production Methods are abundant and diverse, among which microbes have been widely used in Marine Carbohydrate Production due to their advantages of short multiplication time, rapid growth, simple nutritional requirements, and wide substrate spectrum. Here, CD BioGlyco offers services to clients for the production of marine carbohydrates by microbes.
Microbial strain selection and breed
Most of the microbial strains that produce marine carbohydrates are marine algae, but there are some other microbes such as Gram-negative bacteria, archaea, and fungi. We inoculate marine microbes in a culture medium incubate them at specific temperatures, and select the optimal strains for carbohydrate production by adjusting the medium and culture conditions. To maximize the fermentation efficiency of the strains, we optimize the medium experimentally using carbohydrate yield and biomass as indicators.
- Optimization of media formulation: By changing the concentration of various carbon and nitrogen sources and inorganic salts required for microbial growth.
- Optimization of culture conditions: Screening for optimal culture conditions by varying temperature, pH, and time.
Selection of fermentation methods and production of carbohydrates
We use the solid-state fermentation method as well as the liquid fermentation method to ferment the microbial strains and compare the carbohydrate yield to select the best fermentation method.
- Solid-state fermentation: The bacteria are inoculated in a liquid medium for a time to achieve a rapid increase in the number of strains and then transferred to a solid medium for subsequent cultivation. The carbohydrates produced by this method are mainly concentrated in the mycelium and solid medium, which is convenient for product separation.
- Liquid fermentation: Inoculate the bacterium into a liquid medium for cultivation, and then isolate and purify the carbohydrates produced by the bacterium in the components of the liquid medium, to obtain the target products.
In addition, we offer services that utilize marine microbial fermentation to absorb CO2 and convert it into target carbohydrates. Compared to plants, microbes are more efficient at sequestering carbon and are easier to modify artificially. Therefore, we utilize single microbial fermentation, multicellular systems, coupled chemical-microbial systems, and in vitro enzyme catalysis to convert CO2 to target carbohydrates. The types of marine microbes we provide in this process include but are not limited to, microalgae and photosynthetic bacteria.
Marine carbohydrate analysis
We offer aqueous, acid, alkaline, and enzymatic extraction methods for the Purification of Carbohydrates. In addition, to meet the different needs of our clients, we also offer new extraction methods that combine various technologies, such as membrane separation, ultrasonic technology, dual-phase aqueous extraction, high-pressure homogenization, and supercritical fluid extraction.
On the other hand, we offer nuclear magnetic resonance (NMR) and infrared (IR) spectroscopy to characterize carbohydrates and further determine their structure to ensure that our clients' needs are met.
Publication Data
Technology: Ethanol precipitation
Journal: Marine Drugs
IF: 4.9
Published: 2022
Results: In this article, the authors describe a method for the production of extracellular polysaccharides (EPS) from marine microbes and the results of the analysis of EPS. Based on EPS production experiments, ethanol precipitation, and centrifugation of the culture medium, dialysis was found to be a high-quality method for isolating and purifying EPS from marine microbes. Compared to EPS produced by marine bacteria, EPS produced by marine fungi showed monotonous results in terms of monosaccharide composition: marine fungal EPS consisted mainly of neutral monosaccharides, including glucose, mannose, and galactose with different molar ratios.
Fig.1 Structures of EPS from Aureobasidium melanogenum. (Qi, et al., 2022)
Applications
- Marine microbes produce carbohydrates from carbon dioxide using photosynthesis or chemical synthesis, which can be further converted into bioethanol or biodiesel for biofuel production.
- Carbohydrates produced by marine microbes often have antiviral, antimicrobial, and antitumor activities and can be used in drug and nutraceutical development.
- Carbohydrates produced by marine microbes such as alginate (agar, carrageenan, etc.) can be used to develop food thickeners, stabilizers, and gelling agents.
Advantages
- We integrate cutting-edge biological and chemical technologies to provide large-scale production of marine carbohydrates.
- We have a well-established quality control system that ensures the safety and consistency of our marine carbohydrate products to meet our client's requirements.
- We customize our marine carbohydrate production solutions to meet the needs of our clients and help them understand and apply the biological functions of marine carbohydrates.
Frequently Asked Questions
What are the advantages of using microbes to produce carbohydrates over traditional production methods?
- Environmentally friendly: Reduces fossil fuel dependence
- Sustainable: Utilizes renewable resources
- Efficient: Certain microbes have faster growth rates and high yields.
Which marine microbes can be used to produce carbohydrates?
Commonly used marine microbes that can be used for carbohydrate production include some microalgae (e.g., Spirulina and Duchenne), marine bacteria (e.g., Streptomyces maritimus), and certain marine fungi.
CD BioGlyco offers cutting-edge and environmentally friendly technology for the production of carbohydrates from marine microbes for a wide range of applications including food, pharmaceuticals, and biomaterials. We are highly skilled in the Production of Marine Biomolecules to ensure that our client's needs in the field of carbohydrate production are met. Please feel free to contact us if you are interested in our services and we will be happy to provide you with professional advice and support.
Reference
- Qi, M.; et al. Exopolysaccharides from marine microbes: source, structure, and application. Marine Drugs. 2022, 20(8): 512.
