Enzymatic Extraction: Pure, Efficient, Sustainable Technology
CD BioGlyco develops an advanced Marine Biomolecule platform and is committed to helping clients utilize marine resources, explore marine bioactive substances, and develop their applications in medicine, health food, cosmetics, and biotechnology. Extracting and purifying Marine Carbohydrates from the ocean is the basis of marine biomolecule research. We provide clients with unparalleled marine carbohydrate production services with advanced Technologies. Here, we introduce the process of producing marine carbohydrates by enzyme extraction technology.
Enzymatic Extraction of Marine Carbohydrate
Enzymatic extraction uses specific enzymes to promote the breakdown of complex matrices in marine organisms, thereby releasing carbohydrates. We select specific enzymes based on the type of polysaccharide to be extracted and mix them with pre-treated marine biomass (such as algae, marine plants, and marine animals) under optimal pH, temperature, and time conditions to promote the breakdown and release of carbohydrates. Enzymatically extracted carbohydrates are purified using techniques such as dialysis, ultrafiltration, or Chromatography to obtain high-purity products. Products are Characterized using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) to ensure product purity and structure.
Chemical Composition Analysis of Extracts
The neutral sugar content in the marine extracts is determined by the phenol-sulfuric acid colorimetric method, the uronic acid content is quantified by the meta-hydroxy-di-phenyl (MHDP) method, and the protein content is determined by the bicaprylic acid colorimetric method (BCA). High-performance anion-exchange chromatography (HPAEC) is used to determine the composition of carbohydrates in the extracts.
Biological Activity Assay
We provide our clients with comprehensive bioactivity analysis services of marine carbohydrates, such as antiviral activity assays, antimicrobial activity assays, antioxidant activity assays, antitumor activity assays, etc.
Publication
Technology: Enzyme-assisted extraction
Journal: Marine drugs
IF: 4.9
Published: 2015
Results: The authors used three commercial carbohydrases (C1, C2, and C3) and a protease (P1) to extract bioactive substances from Chondrus crispus and Codium fragile and analyzed the components of the extracts. The results showed that the extraction efficiency of commercial proteases and carbohydrates was two to three times higher than that of water extraction, and enzymatic hydrolysis enhanced the recovery of proteins, neutral sugars, uronic acid, and sulfate in the extracts. Meanwhile, the enzymatic hydrolysates of C. crispus and C. fragile showed significant activity against Herpes simplex virus (HSV-1) and have potential application value in the development of antiviral drugs.
Fig.1 Schematic representation of seaweed extraction process. (Kulshreshtha, et al., 2015)
Applications
- Pharmaceutical industry: Some marine polysaccharides have good antiviral and antibacterial properties and can be used to develop potential antiviral and antibacterial drugs.
- Cosmetic industry: Marine carbohydrates have good effects on skin moisturizing and anti-aging, and can be used to develop moisturizers, exfoliants, or anti-aging skin care products.
- Agricultural field: Some marine polysaccharides stimulate plant growth and improve resistance to pathogens, and are used to develop plant growth regulators.
- Biotechnology: Marine carbohydrates are used as scaffold materials for tissue regeneration and cell culture or for the development of biosensors.
Advantages of Us
- We have a variety of technologies for marine carbohydrate production. According to the specific needs of clients, we choose the best technology or combine several extraction methods to ensure the efficiency and yield of extraction.
- With advanced production technology, we provide clients with extraction and purification services for a variety of biomolecules in marine plants, marine animals, and marine microorganisms.
- Our enzymatic extraction operates under mild physical conditions, effectively retaining the functional properties of the extracted carbohydrates.
- We use enzymatic methods to extract marine carbohydrates, reducing the need for harsh chemicals and solvents, thereby reducing pollution to the environment.
Frequently Asked Questions
What are the advantages of enzyme extraction technology?
- Specificity and selectivity: Enzymes are highly specific for their substrates, allowing for selective hydrolysis and minimizing the production of by-products.
- Mild conditions: Enzymatic extractions are typically performed under mild temperature and pH conditions that do not compromise the properties of the polysaccharides.
- Environmentally friendly: Enzymatic extractions reduce the need for harsh chemicals and solvents.
What types of enzymes are typically used in marine carbohydrate extraction?
Commonly used enzymes include cellulase, alginate lyase, and agarase from marine plants, chitinase, protease, and glycosidase from marine animals, and extracellular polysaccharide (EPS) degrading enzymes from marine microorganisms.
CD BioGlyco uses the advantages of enzyme extraction to help clients efficiently extract carbohydrates from marine plants, marine animals, and marine microorganisms. If you have any needs in the production of marine carbohydrates, please feel free to contact us.
Reference
- Kulshreshtha, G.; et al. Enzyme-assisted extraction of bioactive material from Chondrus crispus and Codium fragile and its effect on Herpes simplex virus (HSV-1). Marine drugs. 2015, 13(01): 558-580.
