Elevate Your Research with Crenomytilus grayanus Lectin (CGL) Production Service
At CD BioGlyco, we have advanced protein extraction technology and strict production processes. We provide clients with Marine Biomolecule Production Services, including Marine Carbohydrate Production Service, Marine Glycoprotein Production Service, Marine Lectin Production Service, Marine Glycoside Production Service, and Marine Glycolipid Production Service. As for marine lectin production services, we provide a variety of production services including Algae-derived Lectin, Sponge-derived Lectin, Mollusk-derived Lectin, Arthropod-derived Lectin, Echinoderm-derived Lectin, Protochordate-derived Lectin, and Fishes-derived Lectin.
As a company dedicated to biotechnology research and development and application, we efficiently extract high-purity lectin from C. grayanus. Our production services are carefully designed and strictly controlled in every link from raw material collection, pretreatment, extraction, and purification to analysis.
Raw material pretreatment
- Collection and selection: We collect fresh C. grayanus from reliable marine ecological areas to ensure the freshness and activity of the raw materials. We strictly screen the collected samples and select healthy and uncontaminated individuals as raw materials.
- Washing and crushing: We thoroughly wash the selected raw materials to remove the mud and impurities on the surface. We use mechanical or chemical methods to crush the raw materials into particle sizes suitable for subsequent extraction.
- Drying and preservation: We preliminarily dry the washed and crushed raw materials to remove excess water. We store the raw materials under appropriate temperature and humidity conditions to maintain their biological activity and chemical stability.
Extraction
- Solvent selection: We select appropriate solvents for extraction based on the solubility and stability of CGL. Commonly used solvents include buffer solutions and saline solutions.
- Extraction: We use efficient extraction methods such as mechanical stirring, ultrasonic extraction, or high-pressure extraction. We fully dissolve CGL from the raw materials into the solvent under controlled temperature, pH value, and extraction time.
- Filtration and concentration: We use appropriate filtration equipment to remove solid impurities in the extract. Then we concentrate the extract by evaporation, ultrafiltration, and other methods to increase the concentration of CGL.
Purification
- Chromatographic separation: We use affinity chromatography to perform preliminary CGL separation.
- Gel filtration: We use gel filtration technology to further purify CGL.
- Dialysis and concentration: We use dialysis to remove small molecule impurities that may be introduced during the purification process. Then we concentrate on the purified CGL solution for subsequent analysis and application.
Analysis
- Structural analysis: We use nuclear magnetic resonance (NMR) and infrared spectroscopy (IR) to analyze the structure of CGL.
- Purity analysis: We use high-performance liquid chromatography (HPLC) and mass spectrometry (MS) to analyze the purity of CGL.
- Activity analysis: We verify the biological activity of CGL through in vitro experiments, such as inhibiting the formation of syncytial cells caused by human immunodeficiency virus (HIV-1).
In addition, we provide customized lectin production services to adjust the specifications and performance of the products according to client needs and application scenarios. Our professional team works closely with clients to jointly develop lectin products that meet specific needs.
Publication
Technology: Study on the immunological activity of CGL
Journal: Scientific Reports
IF: 4.996
Published: 2017
Results: The authors demonstrated that GalNAc/Gal-specific lectin from the edible mussel C. grayanus activated immune responses in macrophages and mice. In vitro cell models showed that CGL-induced secretion of tumor necrosis factor-α and interleukin-6 in various types of macrophages and human blood cells. The production of cytokines by CGL was found to be influenced by reactive oxygen species, mitogen-activated protein kinases, protein kinase C-α/δ, and NF-κB. Additionally, in lipopolysaccharide (LPS)-activated macrophages, CGL induced endotoxin tolerance by reducing IRAK2 expression, JNK1/2 phosphorylation, and NF-κB activation.
Fig.1 CGL activated macrophages in an LPS independent manner. (Chernikov, et al., 2017)
Applications of CGL
- CGL can be used for sugar-specific studies because it has a specific binding capacity for N-acetylgalactosamine and galactose.
- CGL can be used to purify or isolate proteins that bind to N-acetylgalactosamine or galactose.
- CGL can be used to study bioactive mechanisms such as microbial-host cell interactions and microbial population behavior.
Advantages
- We use advanced production technology and sophisticated purification processes to ensure that the CGL extracted from C. grayanus is of high purity and quality.
- We use efficient and environmentally friendly extraction and purification technology to quickly and effectively extract CGL from C. grayanus.
- We have a research and development team composed of senior biologists and chemical engineers who have rich professional knowledge and practical experience.
Frequently Asked Questions
What is CGL and what are its applications?
CGL is a specific lectin extracted from the Arctic scallop (C. grayanus). It has unique biological activity and can bind to carbohydrate molecules under specific conditions. This lectin has a wide range of applications in the biomedical field, including as a biological probe to detect specific carbohydrate molecules and as a targeting molecule in drug delivery systems. In addition, CGL has also shown potential in the field of food science.
What are the characteristics of the CGL production service provided by your company?
- Efficient extraction: We use advanced biotechnology and sophisticated extraction processes to ensure efficient extraction of high-purity lectins from Arctic scallops.
- Customized service: We provide customized production services and adjust the specifications and quantities of products according to customer needs.
- Technical support: We have a professional technical team to provide customers with full-range technical support from product selection to application plan formulation.
At CD BioGlyco, our CGL production service adopts advanced technology and a strict quality control system to ensure that we provide clients with high-purity and high-quality CGL products. Please feel free to contact us if you are interested in our CGL production service!
Reference
- Chernikov, O.V.; et al. A GalNAc/Gal-specific lectin from the sea mussel Crenomytilus grayanus modulates immune response in macrophages and in mice. Scientific Reports. 2017, 7(1): 6315.
