Revolutionize Your Studies with Our Halimeda renschii Lectin (HRL) Production Service
CD BioGlyco is an innovative biotechnology company focusing on marine glycobiology. We provide high-quality Marine Biomolecule Production Services, including Marine Lectin Production Services. Among them, HRL production service is a distinctive type of lectin production service. HRL production service. H. renschii is a green algae that grows in coral reef areas. It is rich in a unique lectin and has shown great potential in the fields of biomedicine, biotechnology, and the food industry. We use advanced Technologies for Marine Carbohydrate Production and Technologies for the Purification of Marine Polysaccharides to produce high-quality HRL. Then, we use Marine Carbohydrate Characterization Technology to determine the structural and functional properties of HRL.
Raw material pretreatment
- Washing and drying: The collected H. renschii algae raw materials are strictly washed to remove surface impurities and contaminants, and then low-temperature drying is carried out.
- Crushing: The dried H. renschii algae is processed into a powder suitable for extraction by mechanical crushing equipment.
Extraction
- Solvent extraction: We use an optimized solvent extraction method to extract lectin from the crushed H. renschii powder using a specific solvent. During this process, the concentration of the solvent, the material-liquid ratio, the extraction temperature, and time are carefully controlled to maximize the extraction efficiency and product purity.
- Solid-liquid separation: The extracted mixture is subjected to solid-liquid separation techniques such as centrifugation or filtration to obtain a solution containing lectin.
Purification
- Affinity chromatography: We use the affinity of lectin for a specific ligand and purify it using affinity chromatography. This process can effectively remove impurities in the solution and improve the purity of lectin.
- Ion exchange chromatography: We use ion exchange chromatography to further remove ionic impurities in the solution.
- Ultrafiltration and concentration: We use ultrafiltration technology to remove small molecule impurities in the solution and increase the concentration of lectin through the concentration step.
Characterization
- Purity detection: We use high-performance liquid chromatography (HPLC) and other technologies to detect the purity of lectin.
- MS analysis: We use mass spectrometry technology to analyze the molecular weight and structure of purified lectin.
- Activity assay: We determine the biological activity of lectin, such as antiviral activity and glycan chain binding ability, through in vitro experiments.
Publication
Technology: Isolation and characterization of HRL
Journal: Marine drugs
IF: 5.118
Published: 2017
Results: The authors isolated a novel lectin from the green alga H. renschii, named HRL40. Through hemagglutination-inhibition tests and oligosaccharide-binding experiments, HRL40 exhibited specific binding to high-mannose N-glycans with an exposed (α1-3) mannose residue in the D2 arm of branched mannosides.
Fig.1 Purification of HRL40. (Mu, et al., 2017)
Applications
- Specific recognition and binding: HRL can be used to specifically recognize and bind to high-mannose N-glycans containing exposed (α1-3) mannose residues.
- Molecular interaction studies: HRL can be used to study interactions with specific glycan chains.
Advantages
- Advanced production technology: We have advanced production technology for HRL, which can ensure the high purity, high activity, and stability of the product.
- Customized production service: We provide customized production service for HRL, which can be personalized according to the specific needs of clients.
- Comprehensive technical support: Whether it is a problem during the use of the product or a consultation on the product characteristics, we provide professional answers and help promptly.
Frequently Asked Questions
What functional activities does HRL have?
HRL exhibits significant functional activities. First, HRL exhibits strong anti-influenza virus activity by binding to the virus hemagglutinin with high affinity. This functional activity makes HRL potentially useful in antiviral drug development and virus inhibition strategies. In addition, the specific binding ability of HRL provides the possibility for its application in biotechnology and biomedicine, such as affinity chromatography, separation and purification of specific biomolecules, and biosensor development.
I am interested in the production process of HRL, but I have limited expertise in this area. Could you provide a brief description of the production process?
The production process of HRL generally involves several key steps. First, we extract the desired biomolecule from high-quality H. renschii raw materials. Next, we use advanced purification techniques such as affinity chromatography and ion exchange chromatography to remove impurities and obtain highly pure HRL. Finally, we conduct strict quality control and characterization of the product to ensure that it meets the specific needs of our clients.
CD BioGlyco provides high-quality HRL production services to global clients with excellent technology and quality services. We always adhere to the client demand-oriented approach and constantly innovate the production process to ensure the high purity and biological activity of the products. Please feel free to contact us if you are interested in our HRL production service!
Reference
- Mu, J.; et al. A novel high-mannose specific lectin from the green alga Halimeda renschii exhibits a potent anti-influenza virus activity through high-affinity binding to the viral hemagglutinin. Marine drugs. 2017, 15(8): 255.
