Elevate Your Research with Our Solieria filiformis Lectin Production Service: Precision and Quality You Count on
CD BioGlyco as an innovative enterprise focusing on R&D and services in the field of biotechnology, provides clients with Marine Biomolecule Production Services, including but not limited to Marine Carbohydrate Production Service, Marine Glycoprotein Production Service, Marine Lectin Production Service, Marine Glycoside Production Service, and Marine Glycolipid Production Service. As for marine lectin production services, we provide a variety of production services including Algae-derived Lectin Production Service, Sponge-derived Lectin Production Service, Mollusk-derived Lectin Production Service, Arthropod-derived Lectin Production Service, Echinoderm-derived Lectin Production Service, Protochordate-derived Lectin Production Service, and Fishes-derived Lectin Production Service.
Based on a deep scientific research background and rich production experience, our S. filiformis lectin production service focuses on the efficient extraction and purification of lectin from S. filiformis, a special seaweed.
Raw material preparation and pretreatment
- Raw material selection: We select high-quality S. filiformis with a high concentration of lectin as the raw material for extracting lectin.
- Raw material pretreatment: Before the raw material is extracted, we perform appropriate pretreatment, such as washing, drying, and crushing to optimize the subsequent extraction conditions.
Extraction
- Solvent extraction: We use a specific solvent system to effectively dissolve and extract lectin from S. filiformis by controlling factors such as temperature, pH value, and extraction time.
- Optimizing extraction conditions: We conduct many experiments to determine the best extraction conditions, including solvent concentration, extraction temperature, and time to maximize the extraction rate of lectin.
Purification
- Preliminary filtration: The extract is first filtered to remove large particle impurities and undissolved substances.
- Chromatographic technology: We use advanced chromatography technology, such as gel filtration chromatography or ion exchange chromatography to further purify the extract to isolate high-purity lectin.
- Ultrafiltration and concentration: We use ultrafiltration technology to remove small molecule impurities and concentrate the lectin solution to increase the concentration of the product.
Quality inspection and control
- Activity detection: We use specific bioactivity detection methods to ensure that the extracted and purified lectin maintains its biological activity.
- Purity analysis: Through high-performance liquid chromatography (HPLC), mass spectrometry (MS), and other technical means, the purity of lectin is analyzed in detail to ensure that the product meets high standards.
- Safety assessment: We conduct strict safety assessments on the final product, such as microbial contamination detection and endotoxin detection to ensure the safety of the product.
Publication
Technology: Structural and activity characterization of S. filiformis lectin
Journal: International journal of biological macromolecules
IF: 9.2
Published: 2018
Results: The authors examined two variants of S. filiformis lectin (SfL), known as SfL-1 and SfL-2. The primary structures of these variants consisted of four tandem-repeat protein domains, each containing 67 amino acids. Analysis of the 3D structure predicted that both SfL-1 and SfL-2 were comprised of two β-barrel-like domains created from five antiparallel β-strands. Testing revealed that the mixture of isoforms (SfLs) exhibited an anticancer effect on MCF-7 cells.
Fig.1 The overall structure of SfL-1 (A) and SfL-2 (B). (Chaves, et al., 2018)
Applications
- S. filiformis lectin can be used to study the effects on the growth of specific bacteria.
- S. filiformis lectin can be used to study the anti-cancer effects
- S. filiformis lectin can be used to study its anti-inflammatory and anti-pain properties.
Advantages of Us
- Advanced R&D technology: Our advanced technology ensures the accuracy and efficiency of S. filiformis lectin extraction and production, providing the company with a scientific research environment for continuous innovation.
- High-quality scientific research team: We have a high-quality scientific research team, including highly educated personnel such as doctors and masters, as well as researchers with rich experience.
Frequently Asked Questions
What is S. filiformis lectin?
S. filiformis lectin is a special protein produced by the microorganism S. filiformis, which has a high sugar-binding specificity. This lectin has a wide range of applications in multiple fields, including but not limited to biomedical research, drug development, and as a biorecognition tool in biosensors and bioseparation technologies.
What should I pay attention to when using S. filiformis lectin?
First, please make sure to store lectin under appropriate temperature and humidity conditions to avoid its degradation or inactivation. Secondly, please read the product instructions carefully before use to understand the use and precautions of lectin. During use, please follow the appropriate experimental operating procedures to ensure the accuracy and reliability of the experimental results. If you encounter any problems or difficulties during use, please feel free to contact our technical support team, we will be happy to provide you with help and support.
At CD BioGlyco, our S. filiformis lectin production service is your ideal choice when you are looking for high-quality lectin products. With advanced technology, a professional team, and a strict quality control system, we ensure that the produced lectin products have excellent performance and quality. Please feel free to contact us if you are interested in our S. filiformis lectin production service!
Reference
- Chaves, R.P.; et al. Structural characterization of two isolectins from the marine red alga Solieria filiformis (Kützing) PW Gabrielson and their anticancer effect on MCF-7 breast cancer cells. International journal of biological macromolecules. 2018, 107: 1320-1329.
