Precision Fucosylated Chondroitin Sulfate Characterization for Advanced Research
Fucosylated chondroitin sulfate (FCS) is a glycosaminoglycan with good anti-blood clotting activity, mostly found in sea cucumbers. With advanced Marine Carbohydrate Characterization technology and in-depth experience in the Characterization of Marine Animal Polysaccharides, CD BioGlyco provides high-quality FCS characterization services to clients worldwide.
Structure of FCS
We offer a variety of methods to analyze the structure of the main and branched chains of FCS, such as methylation, nuclear magnetic resonance (NMR), infrared (IR) spectroscopy, and mass spectrometry (MS). The combination of these methods allows for precise analysis of the main and branched chain structure of FCS, including the sugar conformation, glycosidic bond type, and linkage.
Monosaccharide Composition of FCS
FCS from different sources are mainly composed of monosaccharides such as N-acetylglucosamine (GlcNAc), N-acetylgalactosamine (GalNAc), and fucose (Fuc), but they still contain small amounts of mannose (Man), glucose (Glc), and galactose (Gal), among others. Two-dimensional NMR (2D NMR) coupled with MS is an effective method for analyzing the monosaccharide composition of FCS, which provides quantitative and qualitative analysis of the monosaccharides contained in FCS.
Molecular Weight (Mw) of FCS
The Mw of FCS can be measured by gel permeation chromatography (GPC) coupled with MS. By this method, we get the information of average Mw, number average Mw, and Mw distribution of FCS.
Purity of FCS
The purity of FCS is analyzed by size exclusion chromatography (SEC) combined with multi-angle laser scattering (MALC), and the specific FCS purity can be determined based on the signal provided by the chromatography in the MALC.
Anti-blood-clotting Activity
The anticoagulant activity of FCS is measured using an anticoagulation test. The anticoagulant activity of FCS is assessed by incubating FCS with erythrocytes for a while and observing the state of the erythrocytes using a microscope. At the same time, we measure the activated partial thromboplastin time (APTT) and prothrombin time (PT) to accurately analyze the anti-blood-clotting activity of FCS.
Publication Data
Technology: NMR
Journal: Marine Drugs
IF: 4.9
Published: 2016
Results: In this research, the authors analyzed the structure of FCS before and after depolymerization using NMR. The results showed that the backbone structure of FCS did not change throughout the depolymerization process and no loss of sulfate occurred. Next, the authors also measured the anti-blood-clotting activity of FCS. Before depolymerization occurred, the APTT of FCS was 183 IU/mg and it showed excellent anti-blood clotting activity. As the Mw of FCS decreased, its activated partial thromboplastin time and prothrombin time showed a decreasing trend.
Fig.1 The NMR mapping of the FCS depolymerized process. (Li, et al., 2016)
Applications
- Characterization of FCS can be used to thoroughly research the structure-function relationship of FCS and reveal the effects of FCS on cell behavior, especially in cell signaling, cell proliferation, and differentiation.
- Characterization of FCS can be used to design and optimize drug carriers using FCS as a substrate to improve drug bioavailability and targeting.
- The characterization service of FCS can help researchers explore the anti-blood-clotting activity of FCS and thus develop anticoagulant drugs.
Advantages
- With years of background in researching FCS, our team is well-versed in the latest industry dynamics and advances in FCS characterization techniques, providing comprehensive theoretical and practical support to our clients.
- We provide specialized FCS characterization and analysis services according to the specific needs of our clients. We also provide complete and detailed data reports, including structural information, quality analysis, and purity testing.
- We have strict confidentiality agreements to ensure the security and privacy of our client's research data.
Frequently Asked Questions
What is FCS?
FCS is a special type of glycosaminoglycan sulfate that has a structurally similar backbone to common chondroitin sulfate (CS) but is characterized by the attachment of fucose molecules to the backbone. FCS is mainly extracted from some marine organisms, notably sea cucumbers and some marine invertebrates.
What bioactivities does FCS have?
FCS possesses some important biological activities, including anti-blood clotting activity and anti-thrombotic activity, anti-viral activity, anti-inflammatory and immunomodulatory activity, anti-inflammatory properties, and antioxidant activity.
CD BioGlyco has an experienced and dedicated scientific team to provide personalized HS characterization services to our clients. Our team is dedicated to the characterization of various types of marine carbohydrates, such as marine-derived Glycosaminoglycans. Whether your needs are for scientific research or biopharmaceutical R&D, we offer the most appropriate research solutions tailored to meet your specific needs. Please feel free to contact us if you are interested in collaborating or need further information.
Reference
- Li, J.H.; et al. Depolymerization of fucosylated chondroitin sulfate with a modified Fenton-system and anticoagulant activity of the resulting fragments. Marine Drugs. 2016, 14(9): 170.
