Optimize Polysaccharide Purification Effectively
Chromatography purification operates by distinguishing components of a mixture based on their interactions with both a stationary phase and a mobile phase. As the mixture progresses through the stationary phase, the components travel at varying speeds due to differences in size, charge, or specific binding affinities, facilitating their Separation and Purification. Various chromatography techniques provide diverse options for purifying marine polysaccharides, each offering unique benefits and applications. By leveraging these advanced methods, CD BioGlyco ensures high purity and maintains the bioactive properties of Marine Polysaccharides, making them suitable for industrial, pharmaceutical, and nutraceutical uses. Additionally, we offer Marine Biomolecules for clients worldwide.
Chromatography purification of marine polysaccharides includes:
Column chromatography
- Anion-exchange chromatography: Separates polysaccharides based on charge by using a column filled with positively charged beads that attract and bind negatively charged polysaccharides.
- Gel-filtration chromatography (or size-exclusion chromatography): Differentiates polysaccharides by size, with smaller molecules eluting later.
- Cellulose column chromatography: Utilizes cellulose as the stationary phase, separating polysaccharides based on their affinity for cellulose.
- Macroporous resin chromatography: Uses resins with large pores to trap and separate polysaccharides based on size and hydrophobicity.
Affinity chromatography
- Relies on specific binding interactions between polysaccharides and ligands attached to the stationary phase.
Publication Data
DOI: https://doi.org/10.3390/md15120388
Technology: Column chromatography, Anion-exchange chromatography, Size-exclusion chromatography (Gel-filtration), Affinity chromatography
Journal: Marine Drugs
IF: 5.4
Published: 2017
Results: This review provides insights into the isolation, characterization and purification processes of marine algal polysaccharides (MAPs), and also highlighting their various biological activities such as immunomodulatory, antitumor, antiviral, antioxidant and hypolipidemic effects. In order to maximize the potential of MAPs in functional foods and pharmaceuticals, the authors advocate the development of efficient and cost-effective extraction and purification techniques, stringent quality control measures, as well as in-depth studies on their structure-function relationships and specific activation mechanisms.
Fig.1 The extraction, purification, chemical-physical properties, and biological activities of MAPs. (Xu, et al., 2017)
Applications
- Chromatography is crucial for purifying active pharmaceutical ingredients to ensure they are free from impurities and meet regulatory standards.
- Chromatography purification ensures the purity of active ingredients in dietary supplements, enhancing their effectiveness and safety.
- Chromatography purification removes contaminants from water, including heavy metals, organic pollutants, and toxins, ensuring safe drinking water.
Advantages
- Chromatographic techniques allow high purity levels to be achieved, preserving the bioactive properties of marine polysaccharides.
- Our chromatographic methods, including column chromatography, gel filtration, and macroporous resin chromatography, can be tailored to different types of polysaccharides and their specific purification needs.
- Chromatographic methods can handle large quantities of extracts, making them suitable for both lab-scale and industrial-scale purification.
Frequently Asked Questions
What are the challenges associated with chromatography purification of marine polysaccharides?
Challenges include the need for specialized equipment, the potential degradation of sensitive polysaccharides, and the complexity of optimizing conditions for different types of polysaccharides to achieve the desired separation.
How does the choice of chromatography method affect the purity and yield of marine polysaccharides?
The choice of method affects both the purity and yield. For example, size-exclusion chromatography is useful for removing smaller contaminants, while ion-exchange chromatography can target specific charged polysaccharides. The method chosen will depend on the specific polysaccharide's properties and the desired end-use.
At CD BioGlyco, our chromatography purification offers several distinct advantages when it comes to isolating and refining marine biomolecules. These advantages ensure high purity, efficiency, and retention of bioactive properties, making the process highly valuable across various applications. Please do not hesitate to contact us for more information.
Reference
- Xu, S.; et al. Recent advances in marine algae polysaccharides: Isolation, structure, and activities. Marine Drugs. 2017, 15(12): 388.
