Unlock the Secrets of Dermatan Sulfate with Precision and Expertise
Dermatan sulfate (DS) is an important Glycosaminoglycan with a wide range of anti-blood clotting activity, anti-inflammatory, anti-viral, and anti-proliferative activities. DS is found in the extracellular machinery of animals, especially in marine animals. At CD BioGlyco, we have a dedicated Marine Animal Polysaccharide Characterization team to provide custom DS characterization services to clients.
Structural Analysis
We offer a variety of characterization techniques to characterize the structure of DS.
- Infrared (IR) spectroscopy: Scanning the DS sample in a certain wave number range gives us an IR spectrum of DS. Based on the IR spectra, we obtain a comprehensive analysis of the structure of the DS sugar ring, the sugar type, and the position of all functional groups.
- Mass spectrometry (MS): An appropriate amount of DS-degraded oligosaccharide solution is taken for MS analysis, which can effectively detect the disaccharide structure contained in DS.
- Nuclear magnetic resonance (NMR): After dissolving DS in heavy water, NMR is used to test the type of glycosidic bonds it contains and the way the glycosidic bonds are connected.
Spatial Structure Analysis
The spatial structure of DS from different sources causes large differences. To address this, we offer atomic force microscopy (AFM), X-ray diffraction (XRD), and circular dichroism spectroscopy (CD) to determine the three-dimensional structure of DS.
Relative Molecular Weight Determination
Gel permeation chromatography (GPC) is an effective method to detect the relative molecular weight of DS, and we use GPC to analyze the relative molecular weight and molecular weight distribution of DS from different marine animal sources. At the same time, a refractive index detector (RID) is used to detect the results.
Monosaccharide Composition Analysis
DS has monosaccharide structures such as mannose, D-glucuronic acid (GlcA), N-acetyl-D-galactosamine (GalNAc), glucose, galactose, and fucose. We use high-performance liquid chromatography (HPLC) to analyze the monosaccharide composition of DS.
Purity Analysis
We use HPLC to determine the purity of DS. In addition, we offer DS products of different purities according to the needs of our clients.
Publication Data
Technology: Ion exchange chromatography (IEC) and cellulose acetate electrophoresis
Journal: Foods
IF: 4.7
Published: 2023
Results: In this research, the authors extracted and purified DS from the skin (GSB), bones (GCB), and head (GHB) of garfish and characterized and compared the composition of DS from different sites. Utilizing IEC, the authors found that the composition, as well as the content of sulfate, glucuronic acid, and total sugars, did not differ significantly in DS extracted from different fish body parts. Cellulose acetate electrophoresis showed that the molecular weights of DS from skin, bone, and head were 37.85 kDa, 34.41 kDa, and 46.13 kDa, respectively. In addition, the authors measured the in vitro anticoagulant properties of CD using an anticoagulation assay and found that it exhibited potent anticoagulant effects.
Applications
- The DS characterization service can be used in the development of DS-based nutraceuticals, helping to ensure the purity and potency of their ingredients.
- The DS characterization services can help scientists research the functions and mechanisms of DS in biochemistry and cell biology.
- DS can be used in the development of bioactive materials, so in fields such as soft tissue and bone engineering, DS characterization services can be used to assess the biocompatibility and bioactivity of materials.
Advantages
- Throughout the DS characterization service, we maintain transparent communication with our clients and provide timely feedback on the progress of DS analyses to ensure that our clients are kept informed of the status of their projects.
- We have a team of experienced scientists who have mastered advanced Marine Carbohydrate Characterization techniques, providing our clients with cutting-edge information and the latest technical support related to DS.
- We provide custom DS characterization solutions to meet the different needs of our clients. For example, detailed characterization of specific chain lengths, sulphation modes, etc. can be performed according to clients' requirements.
Frequently Asked Questions
What is the difference between DS and chondroitin sulfate (CS)?
DS is a glycosaminoglycan that differs from CS in that DS contains idoacetic acid (IdoA), the C-5 differential isomer of d-glucuronic acid (GlcA).
What biological activities does DS have?
DS is an important glycosaminoglycan that has a variety of biological activities in living organisms. For example, anti-blood clotting activity, anti-inflammatory activity, promotion of tissue repair function, and antiviral activity. These biological activities of DS make it valuable for applications in medicine, pharmacy, biomedical research, and the cosmetic industry.
With a dedicated marine carbohydrate characterization team and comprehensive analytical capabilities, CD BioGlyco provides quality DS characterization services to our clients. We ensure that we provide our clients with the high-quality and multi-faceted DS characterization they need to carry out their research projects. Please feel free to contact us if you are interested.
Reference
- Chikha, S.B.; et al. Composition and anticoagulant potential of chondroitin sulfate and dermatan sulfate from inedible parts of garfish (Belone belone). Foods. 2023, 12(21): 3887.
