Professional and Customized Hyaluronan (HA) Characterization That Exceeding Your Expectations
HA is a natural glycosaminoglycan found in various marine animals. It is involved in some processes such as tissue regeneration, wound healing, and joint lubrication. Characterizing HA is important to understand its properties and applications. CD BioGlyco provides a professional and comprehensive HA characterization service to help analyze its structure, properties, and biological functions. Our research team has extensive hands-on experience in the Characterization of Marine Animal Polysaccharides and customizes detailed characterization protocols based on our client's research objectives.
We use multiple strategies for the characterization of HA and provide detailed pre-sales consultation and post-sales technical support. Combining multiple techniques, we mainly provide the following services, and all experiments are done by us.
Extraction and Purification
Our team has developed processes for extracting and measuring HA from various tissues of biological matrices such as fish, and offers a wide range of HA preparation services. Specific extraction steps vary depending on the sample type and are optimized through. Impurities such as proteins and lipids need to be removed during HA extraction. We remove impurities through digestion, centrifugation, and dialysis to ensure that highly purified HA is obtained.
Characterization
We have extensive experience in the characterization of HA and offer a variety of analytical services for HA quantification, molecular weight, physicochemical properties, viscosity, etc. HA is characterized by a variety of techniques, such as size exclusion chromatography (SEC), and mass spectrometry (MS).
- HA quantification
- Size distribution analysis
- Molecular weight distribution
- Structural characterization
- Coefficient of friction
- Degree of crosslinking
- Rheological characterization
- Surface adhesion/interactions
- Aging behavior
- Others
HA Modification
We modify HA to enhance its chemical and mechanical properties suitable for cell adhesion, joint lubrication, drug delivery, and signaling.
Applicable Samples
- Marine animal matrix samples (tissue samples such as cartilage)
- Purified HA samples
- HA analytical data reference (optionally available)
Delivery of Results
- Overall experimental process, methodology
- Characterized raw data
- Comprehensive analytical report including data interpretation and analysis
Publication Data
Technology: High-performance liquid chromatography (HPLC), capillary electrophoresis (CE), MS, SEC, gel filtration chromatography
Journal: Frontiers in Immunology
IF: 5.695
Published: 2015
Results: This paper describes some methods for detecting the content and size distribution of HA in biological tissues and body fluids. HA has an average molecular weight of about 1000-8000 kDa and is synthesized in the form of high molecular weight polymers. HA of different molecular weights are involved in different physiological activities. Efficient methods for the isolation and characterization of HA and its fragments have been developed and continue to be updated. These methods include HPLC, CE, MS, SEC, gel filtration chromatography, and others. These separation and characterization methods described in this paper guide the characterization and analysis of HA.
Fig.1 Example of molecular mass distribution of HA determined by agarose gel electrophoresis. (Cowman, et al., 2015)
Applications
- Characterization of HA structure and properties plays an important role in understanding its structure-function relationship and studying its role in various biological activities.
- Characterization of HA structure and properties is essential for drug delivery development. It helps in better design of controlled release systems and improves drug stability.
- Characterization of HA structure and properties is essential for the design of HA scaffolds that promote cell growth, tissue regeneration, and wound healing.
Highlights
- Our analytical team has extensive experience in HA analysis and provides valuable insights into HA research.
- We provide detailed HA sample characterization services, including molecular weight determination and structural characterization. We use advanced equipment and techniques to ensure accurate and reliable results.
- We provide detailed and clear analysis reports, including data interpretation and reliable analysis, according to the specific needs of our clients.
Frequently Asked Questions
What properties does HA have?
HA solutions have favorable properties such as non-immunogenicity, biocompatibility, biodegradability, hygroscopicity, bioreactivity, viscoelasticity, and non-thrombogenicity. Based on these properties, it has a wide range of applications in various applications such as food, cosmetics, and biomedicine.
How long does HA characterization usually take?
HA characterization time varies depending on the specific type of analysis required. It usually takes a few business days to a few weeks from the time the samples are sent out to the time the final results are analyzed. We develop a customized test plan after identifying the specific needs of the client and completing the test efficiently.
CD BioGlyco provides a reliable and customized HA characterization service to help gain a comprehensive understanding of the structure-function relationship of HA, which in turn informs its various biomedical, tissue engineering, and other applications. Welcome to contact us for further details of HA characterization if you need this area.
Reference
- Cowman, M.K.; et al. The content and size of hyaluronan in biological fluids and tissues. Frontiers in immunology. 2015, 6: 261.
