Cat. No.: X24-04-YM015

Recombinant Arctic marine bacterium HL-ExoI, E. coli

Synonym: Heat Labile Exonuclease I

Size

2500 U; 50 KU; 500 KU

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Datasheet

Datasheet

MSDS

MSDS

Properties

Description

Recombinant Arctic marine bacterium HL-ExoI, E. coli, is a 3’ – 5’ exonuclease that targets single-stranded DNA, produced through recombinant methods in E. coli. Its activity range spans from 25°C to 37°C, and it can be deactivated by a 1-minute incubation at 80°C or a 15-minute incubation at 60°C.

Unit Definition

One unit of the enzyme catalyzes the release of 10 nmol of acid-soluble nucleotides in 30 minutes at 25°C.

Activity

>50 KU/mg

Buffer

10 mM Tris-HCl; 200 mM NaCl; 10 mM MgCl₂; 1 mM DTT; 0.01% (v/v) Triton X-100; 50% (v/v) Glycerol

Applications

Recombinant Arctic marine bacterium HL-ExoI can be used for degrading ssDNA like primers and oligos, ideal for treating sensitive samples, and valuable for developing novel molecular diagnostics applications.

Storage

Store at -20°C.

For Research Use Only. Not For Clinical Use.

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