Advanced Zone Electrophoresis Purification for Marine Polysaccharides
CD BioGlyco offers leading-edge zonal electrophoretic purification for Marine Polysaccharides, which is a technique for separating and purifying complex polysaccharide mixtures based on their charge and size by applying an electric field to the sample inside a capillary tube, causing the polysaccharides to migrate at varying rates due to their charge-to-mass ratios. It offers high-resolution separations, excellent sensitivity, and fast processing, making it ideal for the separation and quantification of a wide range of marine biomolecules, particularly marine carbohydrates, including agar, carrageenan, fucoidan, etc. With advanced equipment, expert methodology, and Technologies for the Purification of Marine Polysaccharides, CD BioGlyco offers purification services to meet the specific needs of researchers and industry professionals.
To effectively perform zone electrophoresis purification of marine polysaccharides, a series of precise steps are followed to ensure high-purity and well-separated fractions. Here is our standard process of zone electrophoresis purification:
Sample preparation
We extract the marine polysaccharides from their natural sources (e.g., seaweed) using appropriate methods such as hot water extraction or enzyme-assisted extraction.
Preparation of capillary
We clean the capillary tube thoroughly to remove any contaminants. This is done using a series of rinses with water, methanol, and the running buffer.
Loading the sample
We inject the prepared polysaccharide sample into the capillary. This is typically done using either pressure or electrokinetic injection methods.
Running the electrophoresis
We apply an electric field across the capillary. The polysaccharides will migrate through the capillary at different rates depending on their charge-to-mass ratios.
Detection and analysis
As the polysaccharides migrate through the capillary, they are detected by a UV detector or another suitable detection system.
Collection of purified fractions
We collect the separated polysaccharide fractions as they elute from the capillary. This is done manually or using an automated fraction collector. If higher purity is required, further purification steps such as dialysis or additional electrophoresis runs can be performed.
Post-processing
We concentrate the purified polysaccharide fractions if necessary, using techniques such as lyophilization (freeze-drying) or rotary evaporation.
Publication Data
DOI: https://doi.org/10.1080/19476337.2017.1308970
Technology: Capillary zone electrophoresis (CZE)
Journal: Cyta-Journal of Food
IF: 2.7
Published: 2017
Results: The study aims to develop a rapid and simple CZE method for simultaneously separating and determining 11 reducing sugar substances that may be present in honey. The method is also applied to the quantitative analysis of reducing sugars in honey, as well as the extraction and degradation of monosaccharides from honey polysaccharides. The results indicate that the developed CZE method can effectively separate and determine reducing sugars in honey. Under optimized conditions, testing a standard sugar solution with a concentration range of 1.2-140.8 μM yielded good linearity. Analysis of 10 different types of honey revealed that all honey samples contained glucose and maltose, while the content and proportion of other reducing sugars varied depending on the source of the honey. Additionally, honey polysaccharides were found to be mainly composed of xylose and glucose, with no arabinose detected, although some honey polysaccharides contained rhamnose and glucuronic acid. The accuracy and stability of the method were validated through recovery experiments. Finally, the results demonstrate that this method can be used for quality control by reducing sugars in honey.
Fig.1 Typical electropherograms of standard PMP-labeled reducing carbohydrates. (Lu, et al., 2017)
Applications
- Purification of Marine Bioactive Polysaccharides for drug development and therapeutic applications.
- Ensuring high purity and activity of polysaccharides used in the development of health supplements.
- Research and development of marine polysaccharides for various biotechnological applications.
Advantages
- CZE allows for the precise separation of polysaccharides with similar structures by exploiting subtle differences in their charge-to-mass ratios.
- The method is highly efficient, enabling the rapid processing of samples with minimal preparation time.
- Our CZE system can detect and quantify polysaccharides at low concentrations, ensuring accurate results even for trace amounts.
- Suitable for a variety of marine polysaccharides, this method can be tailored to meet specific purification needs.
Frequently Asked Questions
What types of marine polysaccharides can be purified using zone electrophoresis at CD BioGlyco?
CD BioGlyco uses zone electrophoresis to purify a wide range of marine polysaccharides, including but not limited to agar, carrageenan, fucoidan, laminarin, and alginate.
How does CD BioGlyco ensure the quality and reproducibility of the zone electrophoresis purification process?
CD BioGlyco employs several measures to ensure the quality and reproducibility of the zone electrophoresis purification process such as state-of-the-art equipment, standardized protocols, quality control checks, skilled technicians, and so on.
By our zone electrophoresis technology, CD BioGlyco meets the highest standards of purity and quality for marine polysaccharides, supporting diverse applications in research, healthcare, and industry. Please feel free to contact us for more information if you are interested in our service.
Reference
- Lu, Y.; et al. Rapid determination and quantitation of compositional carbohydrates to identify honey by capillary zone electrophoresis. Cyta-Journal of Food. 2017, 15(4): 531-537.
