Specialized Marine Polysaccharide Purification
At CD BioGlyco, we offer expert precipitation purification services for marine polysaccharides, utilizing advanced technologies to achieve high-purity isolation. Our methods encompass quaternary ammonium precipitation, graded precipitation, and alcohol precipitation. These techniques are meticulously tailored to address the specific requirements of our clients, ensuring the utmost quality and functionality of purified polysaccharides for diverse research and industrial applications. Additionally, we provide a range of other Technologies for Marine Polysaccharide Purification, our methods can be used to purify Marine Carbohydrate Production, and isolate Marine Biomolecule can be used in research and industrial applications.
Quaternary ammonium salt precipitation purification of marine polysaccharides
This approach employs quaternary ammonium salts to precipitate polysaccharides from the solution. The salts form complexes with negatively charged polysaccharides, inducing their precipitation. This method is especially effective for purifying acidic polysaccharides such as fucoidan and alginate, which possess negatively charged groups.
Graded precipitation purification of marine polysaccharides
This technique involves the sequential addition of precipitating agents to a solution, causing polysaccharides to precipitate at various stages based on their solubility. It offers precise control over the purity and yield of each fraction, thereby enhancing the quality and functionality of the purified polysaccharides.
Alcohol precipitation purification of marine polysaccharides
This method uses alcohols to decrease the solubility of polysaccharides in a solution. Upon adding alcohol, polysaccharides become less soluble and precipitate out of the solution. This technique is extensively used in purifying various marine polysaccharides, including agar, carrageenan, and fucoidan, and is highly suitable for large-scale purification processes.
Publication Data
DOI: https://doi.org/10.1002/efd2.37
Technology: Membrane filtration, Alcohol precipitation, Column chromatographic purification,
Journal: eFood
Published: 2022
Results: The paper provides a comprehensive guide to the extraction, isolation, purification and structural characterization of polysaccharides from natural resources. It emphasizes the importance of polysaccharides due to their multiple biological functions such as immunomodulation, hypoglycemic, hypolipidemic and antitumor activity. This article outlines methods for extracting polysaccharides using hot water, acids and alkaline aqueous solutions. It also details purification techniques such as membrane filtration, alcohol precipitation and column chromatography.
Applications
- Precipitation methods can be used to purify marine carbohydrate production, ensuring high purity and potency in research of drug development.
- Critical for isolating marine biomolecules used in research and industrial applications, ensuring their activity and stability.
- Purified polysaccharides are employed in the development of scaffolds for tissue engineering, supporting the regeneration of tissues and organs.
Advantages
- Techniques like quaternary ammonium salt precipitation are highly specific for acidic polysaccharides, allowing for the targeted isolation of compounds like fucoidan and alginate.
- Methods such as alcohol precipitation are simple and easy to implement, making them suitable for large-scale applications.
- Precipitation methods can efficiently remove impurities, resulting in high-purity polysaccharide products that are suitable for sensitive applications like pharmaceuticals and nutraceuticals.
Frequently Asked Questions
What is the process of quaternary ammonium salt precipitation purification?
What is the process of alcohol precipitation purification?
These precipitation purification methods, each with its unique principle and advantages, are integral to the effective isolation and purification of marine polysaccharides. At CD BioGlyco, these methods are employed to ensure the highest quality and functionality of purified marine polysaccharides for diverse research and industrial applications. Please feel free to contact us if you are interested in our services and technologies.
Reference
- Huang, X.; et al. Guideline for the extraction, isolation, purification, and structural characterization of polysaccharides from natural resources. eFood. 2022, 3(6): e37.
