Purification of Marine Polysaccharides with Membrane Filtration
At CD BioGlyco, we offer bespoke membrane filtration purification technology for Marine Polysaccharides. This technique is based on selective membranes that allow the selective isolation and purification of polysaccharides because they have different molecular weights and molecular sizes. Smaller molecules, as well as impurities, get filtered as they pass through the membrane, whereas the larger polysaccharides are retained.
Polysaccharide purification by membrane filtration is a very facile approach useful for the isolation of high-purity polysaccharides. It works under mild conditions in which the biological activity of the polysaccharide does not change much. CD BioGlyco uses membrane filtration techniques to purify marine polysaccharides like fucoidan, alginate, carrageenan, and agar to the high purity levels required for different applications. Additionally, we also provide other Technologies for the Purification of Marine Polysaccharides for clients to speed up their research.
To ensure the effective purification of Marine Molecules like polysaccharides, we use membrane filtration, and here are several key steps that must be highlighted throughout the process.
Filtration process
- Introduce the prepared polysaccharide solution into the membrane filtration system.
- Adjust the flow rate and pressure. Typically, the process is run at moderate pressure to push the solution through the membrane.
Monitoring and control
- Monitor the filtration process to check pressure drop, flow rate, and membrane integrity.
- Periodically collect samples from the retentate (retained polysaccharide solution) and permeate (filtered solution) to assess high-purity polysaccharides.
Collection of purified polysaccharide
- Once the desired concentration and purity levels are achieved, collect the reflux solution.
Post-processing
- If necessary, concentrate the purified polysaccharide solution using other filtration or evaporation techniques.
- Dry purified polysaccharides using lyophilization (freeze-drying) or air-drying to obtain the final product in powder form.
Quality assures
- Purified polysaccharides are analyzed by using HPLC, mass spectrometry, or NMR to confirm purity and molecular identity.
Publication Data
DOI: https://doi.org/10.3390/md18030168
Technology: Algal polysaccharides (APS) extraction, Ethanol precipitation, Membrane separation, Ion exchange, Size exclusion, Affinity chromatography
Journal: Marine Drugs
IF: 5.4
Published: 2020
Results: In addition to differences in molecular weight, monosaccharide composition, and sulfate content, extracted APS are often contaminated with proteins and low molecular weight compounds that dissolve in water during the extraction process. Therefore, additional purification steps are required, using methods such as ethanol precipitation, membrane separation, ion exchange, size exclusion, and affinity chromatography. The choice of purification technique depends on the desired purity of the polysaccharide and the intended use, which means that there is no universal solution. In the functional food industry, ethanol precipitation is a common method, while membrane separation is more suitable for scale-up testing.
Applications
- Purified polysaccharides by membrane filtration can be used to produce biopolymers, which are used in a variety of biotechnological processes and products.
- Purified polysaccharides by membrane filtration can be used as excipients and active ingredients in pharmaceutical formulations.
- Purified polysaccharides by membrane filtration can be used in dietary supplements, where membrane filtration ensures greater bioactivity of the polysaccharides.
Advantages
- Membrane filtration technology can remove impurities effectively to obtain high-purity polysaccharide products.
- The membrane filtration process is gentle and does not involve high temperatures or violent chemical reactions, maximizing the biological activity and structural integrity of the polysaccharide.
- Membrane filtration technology is capable of rapidly processing large volumes of polysaccharide solutions, so it is suitable for industrial-scale production.
- Membrane filtration relies mainly on physical separation mechanisms, which reduces the use of chemical reagents and is more environmentally friendly.
Frequently Asked Questions (FAQs)
What are the key benefits of using membrane filtration for the purification of marine polysaccharides?
There are several advantages of purifying marine polysaccharides by membrane filtration. First, it allows to obtaining of high-purity polysaccharides, and also the process is gentle enough to preserve the biological activity and structural integrity of polysaccharides. In addition, membrane filtration applies to a wide range of polysaccharides.
How does membrane filtration work in the purification of marine polysaccharides?
The membrane filtration process can be divided into sample preparation, selection of suitable membranes, filtration operation, process detection and control, and finally collection of purified polysaccharides. If necessary, the purified polysaccharides can also be concentrated.
CD BioGlyco provides purification services based on our membrane filtration purification technology, ensuring high-purity isolation of marine polysaccharides like fucoidan, alginate, carrageenan, agar, and so on. Please feel free to contact us if you have any questions or concerns about our services and technologies.
Reference
- Dobrinčić, A.; et al. Advanced technologies for the extraction of marine brown algal polysaccharides. Marine drugs. 2020, 18(3): 168.
