Purifying Marine Polysaccharides with Precision Based on Metal Coordination
CD BioGlyco has a metal-coordination purification technology for Marine Polysaccharides that utilizes the ability of various metal ions (e.g., copper, barium, calcium, and lead) to form coordination complexes with polysaccharides and precipitate them from solution. By selecting the appropriate metal ions and conditions, it is possible to target specific Valuable Compounds for purification, making the method particularly effective for complex mixtures. In addition, we offer other Marine Polysaccharide Purification Technologies for clients to choose from.
Coordination chemistry fundamentals
Polysaccharides can interact with metal ions through their hydroxyl (-OH), carboxyl (-COOH), and other functional groups. These interactions form coordination bonds where the polysaccharide acts as a ligand, and the metal ion serves as the central atom. When metal ions are introduced to a solution containing polysaccharides, they form stable complexes with the polysaccharide molecules.
Selective precipitation
Different polysaccharides have varying affinities for different metal ions. By carefully selecting the metal ion, it is possible to selectively precipitate a specific polysaccharide while leaving others in the solution.
Our purification process is as follows:
- Extraction of polysaccharides: Polysaccharides are first extracted from marine sources (such as seaweed) using methods like hot water extraction or enzyme-assisted extraction.
- Addition of metal ions: The extracted polysaccharide solution is treated with a solution of metal ions. Commonly used metal ions include copper (Cu2+), barium (Ba2+), calcium (Ca2+), and lead (Pb2+).
- Formation of metal-polysaccharide complexes: The metal ions interact with the functional groups on the polysaccharides, forming stable, insoluble metal-polysaccharide complexes.
- Precipitation and separation: The metal-polysaccharide complexes precipitate out of the solution due to their reduced solubility. These precipitates are then separated from the solution through filtration or centrifugation.
- Washing of precipitates: The precipitated complexes are washed with solvents (such as water or ethanol) to remove any unbound metal ions and other impurities.
- Decomposition of complexes: The metal-polysaccharide complexes are decomposed using acidic solutions, which release the purified polysaccharides and dissolve the metal ions back into the solution.
- Recovery and drying: The purified polysaccharides are recovered from the solution, typically by filtration or evaporation, and then dried to obtain the final product.
Publication Data
DOI: https://doi.org/10.3390/polym15112511
Technology: FTIR Spectroscopy, UV-Vis spectroscopy, Magnetic susceptibility measurements, Thermogravimetric analysis, X-ray diffraction
Journal: Polymers
IF: 4.6
Published: 2023
Results: This paper focuses on the synthesis and characterization of a novel cross-linked biopolymer made from sodium alginate (AG) and carrageenan (CAR). The biopolymer complexes with a variety of metal ions including Mn(II), Fe(III), Ni(II), and Cu(II). This study emphasizes the significant antimicrobial, antioxidant and anticancer activities of these metal-polymer complexes. The synthesized complexes were characterized using various techniques such as FTIR, UV-Vis spectroscopy, magnetization, thermogravimetric analysis, and X-ray diffraction. The biological activities were evaluated by antimicrobial tests against various strains of bacteria, an antioxidant assay using the DPPH free radical scavenging method, and an anticancer assay against human breast cancer cells (MCF-7) and normal mammary epithelial cells (MCF10A). The results showed that these complexes have favorable bioactivity, making them potential candidates for medical biomaterial applications.
Fig.1 X-ray diffraction patterns of the different prepared polymeric complexes. (El-Ghoul, et al., 2023)
Applications
- This method can be used for selective purification of specific polysaccharides based on their affinity for particular metal ions.
- This method can effectively remove impurities, leading to high-purity polysaccharide products.
Advantages
- The formation of metal-polysaccharide complexes contributes to the effective removal of impurities to obtain polysaccharide products of high purity.
- Metal coordination stabilizes the structure of polysaccharides and improves their bioavailability, thereby enhancing the biological activities of polysaccharides, such as antioxidant, antimicrobial, and anticancer properties.
- Metal coordination applies to a wide range of marine polysaccharides and is a versatile purification technique.
Frequently Asked Questions
What types of marine polysaccharides can be purified using metal coordination purification?
Metal coordination purification is highly versatile and can be used to purify a wide range of marine polysaccharides, including fucoidan, alginate, carrageenan, and agar. This method is particularly effective for complex mixtures.
How does metal coordination purification enhance the biological activities of marine polysaccharides?
Metal coordination purification enhances the biological activities of marine polysaccharides by forming stable metal-polysaccharide complexes. These complexes can improve the structural stability and bioavailability of the polysaccharides, leading to enhanced antioxidant, antimicrobial, and anticancer properties.
CD BioGlyco offers a robust and efficient method for the purification of marine polysaccharides, ensuring their high purity and retaining their bioactive properties. Please feel free to contact us for more details if you are interested in our technologies and services!
Reference
- El-Ghoul, Y.; et al. Synthesis and characterization of a new alginate/carrageenan crosslinked biopolymer and study of the antibacterial, antioxidant, and anticancer performance of its Mn (II), Fe (III), Ni (II), and Cu (II) polymeric complexes. Polymers. 2023, 15(11): 2511.
