Unleashing the Power of Nature: Premium Cinachyrella Galectin Production
Galectins are a kind of lectin that can recognize glycans containing β-galactose structure and is widely distributed in different kinds of organisms. Galectins have various and rich physiological activities, especially in the immune system. The special living environment and physiological structure of marine organisms make them an important source of lectins. Among them, Lectins from Sponges have favorable biological activity, such as anti-inflammatory and promoting cell recognition.
CD BioGlyco has in-depth experience in the field of Lectin Production and offers high-quality Cinachyrella galectin production service to clients with state-of-the-art lectin production and characterization technologies.
Extraction of Cinachyrella galectins
An aqueous extract of Cinachyrella is obtained by making a homogenate of the Cinachyrella sample with water and then lyophilizing it. The aqueous extract is then filtered through a cellulose membrane and dialyzed using water, and the substance obtained after dialysis is lyophilized to obtain crude Cinachyrella galectins.
Isolation and purification of Cinachyrella galectins
Thecrude Cinachyrella galectins are initially purified on an ion-exchange column, and then the galectin fraction is further purified using an affinity chromatography column. In this process, tris-buffered saline (TBS) buffer is used to elute the proteins bound to the chromatography column from the galectin crude.
The product purified by affinity chromatography is subsequently purified in reversed-phase high-performance liquid chromatography (RP-HPLC), an operation that efficiently separates lactose-affinity proteins. Finally, the galectin fraction is collected and the solvent is removed to obtain the pure galectin product.
Throughout the process, we use sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to monitor the purity of galectins.
Analysis of Cinachyrella galectins
- Structural characterization
We offer infrared (IR), nuclear magnetic resonance (NMR), and mass spectrometry (MS) to examine the primary and secondary structure of Cinachyrella galectins. In addition, the molecular ionic structure of Cinachyrella galectins can be accurately measured using, matrix-assisted laser desorption ionization- time-of-flight MS (MALDI-TOF-MS).
- Molecular weight detection
- Size exclusion chromatography offers a rough estimate of the molecular weight size of Cinachyrella galectins.
- SDS-PAGE (using Caumas Brilliant Blue staining) and MS are two methods for efficiently measuring the molecular weight of Cinachyrella galectins.
- Haemagglutination activity assay
Cinachyrella galectin is diluted with a specific buffer and tested for its haemagglutination activity in well plates. We add diluted Cinachyrella galectin, and erythrocyte suspension sequentially to the well plate and incubate it for a while to observe its haemagglutination activity under the microscope.
In addition, in the haemagglutination assay, we test the haemagglutination inhibition of Cinachyrella galectin by adding different kinds of glycans to it.
- Cinachyrella galectin stability assay
- The pH stability of Cinachyrella galectins can be tested by diluting Cinachyrella galectins with different pH buffers and performing a haemagglutination assay.
- The temperature stability of Cinachyrella galectins can be tested by performing a haemagglutination test at different temperatures.
Publication Data
Technology: Affinity chromatography and X-ray crystallography
Journal: Marine Drugs
IF: 5.4
Published: 2015
Results: In this article, the authors describe the purification and analysis of Cinachyrella galectins. The galectins were efficiently purified from Cinachyrella using affinity chromatography, and X-ray crystallographic analysis revealed that Cinachyrella galectins possess a stable tetrameric structure formed by two sequences. In addition, Cinachyrella galectins were found to be active at high temperatures.
Applications
- Cinachyrella galectins are involved in various physiological processes of sponge cell adhesion, proliferation, and apoptosis, and can be used to regulate cell growth, differentiation, and migration processes.
- Cinachyrella galectins have specificity for glycan binding, so they can be used to detect specific carbohydrates in tissues.
- Cinachyrella galectins are stored in sponge ball cells and sponge fibers and can be used to regulate sponge fiber production.
Advantages
- We have a professional and excellent Marine Biomolecule Production team, constantly exploring new biological and chemical technologies, and continue to provide high-quality Cinachyrella galectin production solutions to our clients.
- We offer fully custom Cinachyrella galectin production solutions and lectin products according to the different needs of our clients.
- Our team consists of researchers working in Cinachyrella galectins and provides a full range of technical support to our clients.
Frequently Asked Questions
What types of cells can express galectins?
Galectins are expressed by activated T and B cells, regulatory T cells, dendritic cells, mast cells, eosinophils, monocytes/macrophages, and neutrophils because one of the galectins' main functions is to regulate the immune and inflammatory response.
What are the characteristics of galectins?
Galectins are found only in the animal kingdom and have preferential binding selectivity for galectin residues. Based on structural variability, galectins are classified into 3 subfamilies:
- Prototypic galectins: They are non-covalent dimers, each with two identical carbohydrate recognition structural domains.
- Chimeric galectins: They have two distinct structural domains that may bind the non-glycan portion to the glycan portion.
- Tandem repeat galectins: They have two carbohydrate-recognizing structural domains linked by a functional linker peptide.
CD BioGlyco provides a one-stop solution for Cinachyrella galectin production, including lectin extraction, isolation and purification, structural characterization, and activity analysis. We guarantee to provide reliable and first-class services to help our clients precisely explore the applications of lectins from different marine organisms. Please feel free to contact us for more marine lectin-related information.
Reference
- Gardères, J.; et al. Porifera lectins: diversity, physiological roles, and biotechnological potential. Mar. Drugs. 2015, 13(8): 5059-5101.
