Maximize Your Research Potential with Pellina semitubulosa Lectin (PSL) Production
CD BioGlyco is an innovative enterprise dedicated to the research development and application of cutting-edge biotechnology. We provide high-quality Marine Biomolecule Production Services, such as Marine Lectin Production Services.
PSL is a unique marine bio-lectin. Its strong specific binding ability and biological activity make it show great application potential in the fields of biomedicine, immunodiagnosis, and cell biology. Relying on advanced Technologies for Marine Carbohydrate Production, Technologies for the Purification of Marine Polysaccharides, and Marine Carbohydrate Characterization Technology, we successfully achieve the efficient production of PSL and convert it into a series of high-quality products to meet the needs of scientific research and industrial applications.
Pretreatment
Before extracting PSL, the raw sponge needs to be pretreated. This includes removing impurities from the sponge surface, washing, and drying to ensure the purity and suitability of the raw material. During the pretreatment process, we strictly control the operating conditions to ensure that there is no adverse effect on the activity of lectin in the raw material.
Extraction and purification
Extraction is a key step in PSL Production Service. We use an optimized extraction method to maximize the extraction of lectin from the sponge by adjusting parameters such as solvent type, concentration, pH value, and temperature. During the extraction process, we strictly monitor various parameters to ensure the maximization of extraction efficiency and lectin activity.
The purification step aims to remove impurities in the extract and improve the purity of lectin. We use a variety of purification techniques, such as centrifugation, filtration, and chromatography to achieve efficient purification of lectin. The purity of purified lectin is as high as more than 95%, and it maintains good activity and stability.
Characterization
To ensure the quality and performance of PSL, we conduct a comprehensive characterization analysis.
- Molecular weight analysis: We determine the molecular weight of lectin by mass spectrometry (MS) and other techniques.
- Specificity analysis: We use agglutination experiments and other methods to detect lectin-specific recognition and binding ability to glycans such as D-galactose and L-arabinose.
- Red blood cell precipitation experiment: We verify the precipitation ability of lectin through red blood cell precipitation experiments and determine its precipitation effect on red blood cells of different blood types.
- Structural analysis: We use X-ray crystallography, nuclear magnetic resonance (NMR), and other techniques to analyze the three-dimensional structure of lectin.
Publication
Journal: Marine drugs
IF: 5.118
Published: 2015
Results: The authors provide an overview of 39 lectins from the phylum Porifera, which includes Pellina semitubulosa lectin. Their purification from crude extracts was primarily carried out through affinity chromatography and gel filtration techniques. Additional methods were developed to study sponge lectins, such as screening sponge genomes and expressing them in heterologous bacterial systems. The characterization of the lectins was achieved through Edman degradation or mass spectrometry.
Applications of PSL
- Studying cell recognition and adhesion mechanisms: PSL can be used as a research tool to explore the interaction between cell surface glycan molecules and lectins.
- Analyzing the glycan chain structure of glycoproteins: Since PSL has a high affinity for specific glycans, it can be used as a probe in affinity chromatography or affinity electrophoresis to separate and identify specific glycan chain structures in complex glycoprotein mixtures.
- Studying immune responses and inflammatory responses: As an immune-related protein, PSL can be used to study the function and regulatory mechanism of the immune system.
Advantages
- Technological leadership and R&D strength: We have a professional R&D team with a deep biotechnology background and rich R&D experience. We are constantly pursuing technological breakthroughs to ensure that we maintain technological leadership in the production process of PSL.
- Flexible customized services: Clients can choose products of different specifications and purity according to their needs.
- Professional technical support and after-sales service: Our technical team provides clients with professional technical guidance. At the same time, we provide clients with timely after-sales service to solve problems and difficulties encountered by clients during use.
Frequently Asked Questions
What functional activities does PSL have?
PSL has significant functional activities, mainly including the ability to specifically recognize and bind D-galactose and L-arabinose. In addition, the substance is also able to precipitate human red blood cells, such as red blood cells of blood types A1, A2, A1B, B, and O. These functional activities indicate that PSL may play an important role in cell recognition and adhesion mechanisms.
What customization options does your PSL production service have?
We provide flexible customization services to meet the needs of different clients. Clients can choose PSL of different specifications and purity according to their application scenarios and specific requirements.
CD BioGlyco successfully transforms the potential of P. semitubulosa into high-value PSL products. We strictly control every step from pretreatment, extraction, and purification to characterization to ensure the high purity, high activity, and high stability of PSL. Please feel free to contact us if you are interested in our PSL production service!
Reference
- Gardères, J.; et al. Porifera lectins: Diversity, physiological roles and biotechnological potential. Marine drugs. 2015, 13(8): 5059-5101.
