Take Your Studies to New Heights with Our Suberites domuncula Lectin (SDL) Production Service
CD BioGlyco focuses on the cutting-edge field of marine biotechnology. We provide Marine Biomolecule Production Services to explore and utilize the unique biomolecules of marine organisms. As an important part of this, we specifically provide Marine Lectin Production Services, among which S. domuncula lectin production service is one of the highlights. We use advanced Technologies for Marine Carbohydrate Production, Technologies for the Purification of Marine Polysaccharides, and Marine Carbohydrate Characterization Technology to provide clients with high-quality SDL products to meet various scientific research and industrial application needs.
Raw material collection and pretreatment
We collect samples from the marine organism S. domuncula. The collected raw materials undergo fine pretreatment steps such as washing, crushing, and filtering to remove impurities.
SDL extraction and purification
- Extraction: We use advanced bioseparation technologies such as ultrasound-assisted extraction and enzymatic extraction to extract SDL from the pretreated raw materials efficiently and gently.
- Purification: We effectively remove impurities through multi-step purification processes such as ion exchange chromatography, gel filtration chromatography, and affinity chromatography.
Quality control and testing
We use advanced analytical techniques such as high-performance liquid chromatography (HPLC), mass spectrometry (MS), and nuclear magnetic resonance (NMR) to conduct comprehensive testing of the purity, activity, and structure of SDL.
Product packaging and delivery
- Product packaging: We use professional packaging materials and packaging technology to carefully package the products. The packaging materials meet environmental protection and safety standards to ensure the stability and safety of the products during transportation and storage.
- Delivery method: We select the appropriate transportation method and transportation company to ensure that the product can be delivered to the client on time and safely. During the transportation process, we will monitor and track the product in real time to ensure the safety and reliability of the product.
Publication
Journal: Journal of Biological Chemistry
IF: 4.8
Published: 2003
Results: The authors demonstrated the existence of a tachylectin-related protein in the demosponge S. domuncula, referred to as Suberites lectin. The cDNA that encodes the S. domuncula lectin was successfully identified and cloned, revealing that it consists of 238 amino acids in the open reading frame. The resulting protein was found to have one potential transmembrane region, three characteristic Cys residues, and six internal tandem repeats. The natural sponge lectin was then purified using affinity chromatography, with a size of 27 kDa, and showed antibacterial action against both Gram-negative bacteria like Escherichia coli and Gram-positive bacteria such as Staphylococcus aureus.
Fig.1 Expression of SDLEC in different specimens from S. domuncula. (Schröder, et al., 2003)
Applications of SDL
- SDL can be used to study the mechanism of enzymatic reactions, especially the unique enzyme system found in sponges.
- SDL can be used to study the synthesis mechanism of sponge biomaterials.
- SDL can be used for surface modification of biomaterials taking advantage of the specificity of SDL binding to glycans.
Advantages
- We use advanced bio-separation and purification technologies to efficiently extract SDL from S. domuncula while maintaining its biological activity and structural integrity.
- Our production process is carefully designed and optimized to maximize the stability of SDL biological activity during production and storage by using appropriate buffers and temperature control.
- Our SDL production technology is highly flexible and can be adjusted according to the needs of different clients.
Frequently Asked Questions
How do you ensure the purity and activity of SDL?
With our professional quality control team and advanced production technology, we conduct strict purity and activity tests on each batch of SDL produced. Through precise analysis and evaluation, we ensure that the purity of SDL meets the highest standards and maintains its biological activity. In addition, we also adopt appropriate storage and transportation conditions to ensure that SDL is in the best condition when it is delivered to clients.
What does your technical support include?
Our technical support services cover many aspects. First, we provide clients with detailed product usage guides and operating instructions to ensure that clients can use SDL products correctly. Secondly, our professional team provides clients with technical consultation and solution support to help clients solve problems encountered during use. In addition, we communicate with clients regularly to understand the use of products and make product improvements and optimizations based on client feedback. Our goal is to provide clients with a full range of technical support to ensure that clients can make full use of SDL products and achieve the best results.
At CD BioGlyco, our S. domuncula lectin production service provides clients with high-quality SDL products with advanced biotechnology and a strict quality control system. We are committed to providing clients with comprehensive technical support and high-quality after-sales service to ensure that clients can make full use of SDL products and achieve the best results. Please feel free to contact us if you are interested in our SDL production service!
Reference
- Schröder, H.C.; et al. Emergence and disappearance of an immune molecule, an antimicrobial lectin, in basal metazoa: a tachylectin-related protein in the sponge Suberites domuncula. Journal of Biological Chemistry. 2003, 278(35): 32810-32817.
