Empowering Research Through High-Quality Cliona varians Lectin Production
Sponge extracts can agglutinate erythrocytes and have a specific agglutinating effect on human erythrocytes. Therefore, in-depth research on Sponge-derived Lectin is a topic of great biological importance. As a result of research, it has been shown that lectin from the marine sponge Cliona varians is a tetrameric glycoprotein, linked by disulfide bonds, which has a good physiological defense effect.
With superior Marine Biomolecule Production skills, CD BioGlyco has won a lot of accolades in the field of Marine Lectin Production. Here, we provide professional Cliona varians lectin production service to our clients.
Extraction of Cliona varians lectin
After clipping the sponge Cliona varians, a buffer solution is used to extract lectin directly. The pH of the buffer is usually between 7-8, which will produce an osmotic shock to the Cliona varians cells, thus disrupting the cell membrane and extracting the lectin molecules.
To enrich lectins, we offer precipitation methods. Among other things, Ca2+ precipitation helps to promote the enrichment of lectins that are structurally dependent on Ca2+. Acetone precipitation is also an effective method for concentrating proteins in extracts.
Purification of Cliona varians lectin
Purification of Cliona varians lectin is mainly carried out by chromatography. Here, we offer a variety of methods for purifying Cliona varians lectin for clients to choose from.
- Affinity chromatography (AC): Sponge lectins have a strong affinity for galactoside residues, and AC columns using agarose or lactose binding matrices can effectively purify Cliona varians lectin.
- Gel filtration chromatography (GFC): GFC not only separates lectins of different molecular weights but also estimates their relative molecular weights.
- In addition, we offer a combination of hydrophobic interaction chromatography (HIC) and ion exchange chromatography (IEC) for the purification of Cliona varians lectin.
Characterization of Cliona varians lectin
Gel electrophoresis and gel permeation chromatography (GPC) can be used to detect the multilevel structure of Cliona varians lectin.
Mass spectrometry (MS) can be used to detect the subunit structure of Cliona varians lectin.
We utilize sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and GPC to determine the subunit molecular weight and overall relative molecular mass of the subunit of Cliona varians lectin.
- Haemagglutinating activity
Diluted Cliona varians lectin and erythrocyte suspension are gradually added to the microtiter plate and incubated at room temperature for a while, and then the haemagglutinating activity of lectin is observed under the microscope.
Publication Data
Technology: SDS-PAGE and size exclusion chromatography (SEC)
Journal: Anais da Academia Brasileira de Ciências
IF: 2.3
Published: 2015
Results: In this article, the authors extracted Cliona varians lectin CvL-1 and CvL-2 using acetone precipitation, purified them by SEC, detected the molecular weights of CvL-1 and CvL-2 by SDS-PAGE and SEC, and also screened for haemagglutination activity. The results showed that CvL-2 is a trimeric protein with a subunit of 175 kDa. it was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. In addition, CvL-2 showed toxicity to Artemia naplii and the LC 50 of CvL-2 was 850.1 μg/mL.
Fig.1 Size exclusion chromatograms of CvL-1 and CvL-2. (Moura, et al., 2015)
Applications
- Cliona varians lectin inhibits cell proliferation and promotes apoptosis through different pathways, thus acting on cancer cell lines. Thus, it can be used to separate cancer cells from normal cells by its specific binding to non-solid tumors.
- Cliona varians lectin has antiviral activity and can be used to inhibit the production of viral antigens and prevent viral cytopathy.
- Cliona varians lectin can be used as an immunomodulatory molecule in vertebrate cells to combat diseases that lead to immune system failure.
Advantages
- We provide custom Cliona varians lectin production services and Cliona varians lectin products according to our clients' R&D needs.
- Our R&D team is proficient in marine lectin production technology and provides all-around technical support to our clients to help them better apply Cliona varians lectin products.
- We provide one-stop Cliona varians lectin production services covering lectin extraction, purification, and characterization to improve efficiency and save time for our clients.
Frequently Asked Questions
What types of sponge lectins exist?
Classified according to the primary structure of the lectin, sponge lectins can be divided into galactose lectins, C-type lectins, tachykinin-like lectins, and F-type lectins.
What is the physiological role of sponge lectins?
- Sponge lectins exhibit carbohydrate-binding properties that create molecular bridges between molecules and activate cell pathways, thereby triggering different cellular responses.
- Sponge lectins intermediate sponge cell-cell interactions and promote tissue organization and intercellular adhesion.
- With antibacterial activity against prokaryotes and cytotoxic activity against eukaryotic parasites, several sponge lectins are shown to be involved in sponge defense, defending sponges from predation by unicellular protozoa and marine animals.
CD BioGlyco has a dedicated marine lectin research and development team that is committed to maintaining the highest standards and providing custom Cliona varians lectin production solutions for each client. Please feel free to contact us for any of your project needs and we will continue to provide our full support.
Reference
- Moura, R.M.; et al. Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea. Anais da Academia Brasileira de Ciências. 2015, 87(2): 973-984.
