Unlocking Nature's Healing Power with Haliclona caerulea Lectin Production
Sponges are rich biobanks of biomolecules, especially lectins. These Lectins from Marine Sponges are of interest for their potential biological effects on human cells. At CD BioGlyco, we have mastered advanced production methods for Marine Biomolecule Production. We not only provide marine Marine Lectin Production Solutions to researchers from all walks of life but also offer high-quality lectin products.
Here, we offer Haliclona caerulea lectin production solutions as shown below.
Extraction of Haliclona caerulea lectin
The marine sponge Haliclona caerulea is cut and ground into powder, followed by acetate buffer to extract the lectin. After some time, the mixture of buffer and Haliclona caerulea is filtered and centrifuged at low temperature, followed by collection of the supernatant and freeze-drying to arrive at the crude Haliclona caerulea lectin.
Isolation and purification of Haliclona caerulea lectin
Haliclona caerulea lectin is a biomolecule, and we purify this lectin using a combination of affinity chromatography and ion exchange chromatography.
In this process, we use a specific buffer as the mobile phase, and the absorbance magnitude of the eluent is used as a reference to collect the eluent. The collected lectin-containing eluent is dialyzed and freeze-dried to obtain high-purity lectin.
Characterization of Haliclona caerulea lectin
The molecular weight of lectin is detected using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), gel filtration, and size exclusion chromatography.
- Structural characterization
- Primary structure and amino acid sequence of Haliclona caerulea lectin by tandem mass spectrometry (MS/MS)
- Detection of the three-dimensional structure of Haliclona caerulea lectin by X-ray crystallography
- Sequencing of lectins using Edman degradation and MS
- Hemagglutination and inhibition assay
The haemagglutination test for Haliclona caerulea lectin is performed in microtiter plates. We dilute the lectin with saline and add it to the microtiter plate, followed by adding diluted erythrocytes from different animal sources to each well and observing the haemagglutination activity of the lectin under a microscope or with the naked eye.
Following the same approach, we also offer a haemagglutination inhibition assay for lectins by carbohydrates.
Applications
- Haliclona caerulea lectin is toxic to certain tumor cells, so it can be used to develop anti-tumor drugs.
- Haliclona caerulea lectin can bind certain glycans to cell surfaces, thereby recognizing cancer cells and localizing tumor cells.
- Haliclona caerulea lectin can be used to regulate cell aggregation, symbiosis, and defense and growth processes.
Advantages
- Our research team utilizes state-of-the-art biological and chemical technologies to produce high-purity Haliclona caerulea lectin for applications such as scientific research and pharmaceutical research and development.
- We offer custom production solutions to meet the different requirements of our clients for Haliclona caerulea lectin.
- Our experienced team with rich production experience and expertise ensures the quality and stability of Haliclona caerulea lectin production.
Frequently Asked Questions
How can we identify whether a protein is a lectin or not?
A protein can be classified as a lectin if it possesses all three characteristics: it has a carbohydrate recognition domain (CRD), it is of non-immune origin, and it is not catalytically active.
What are the biological activities of lectins from sponges?
Research has shown that lectins from sponges generally have the following biological activities: mitogen activity, cell binding properties, chemotaxis, cytokine production, pro-inflammatory activity, toxicity, recognition of Leishmania protozoa, and ion channel modulation.
At CD BioGlyco, our team of professionals utilizes best-in-class technology to produce high-quality Haliclona caerulea lectin. We are committed to coming up with the perfect solution according to our clients' scientific needs. Please feel free to contact us if you would like to know more about Haliclona caerulea lectin.
Reference
- Gomes Filho, S.M.; et al. Marine sponge lectins: actual status on properties and biological activities. Molecules. 2015, 20(1): 348-357.
