Harvesting Haliclona manglaris Lectin Power, Tailored for You
The marine sponge is a multi-cellular, non-stalked organism of filtration that has tremendous biotechnological possibilities. Also, marine sponges are good sources of Marine Biomolecules such as nucleosides, alkaloids, glycolipids, and lectins. Sponge-derived Lectin shows many important biological activities such as mitogenic activity, cytotoxicity as well as antimicrobial and anticancer activities.
Haliclona genus is the group of marine sponges from which the largest amount of lectins has been isolated. Here, with the world's leading Marine Lectin Production technology, CD BioGlyco offers a one-stop solution for the extraction, purification, and characterization of Haliclona manglaris lectin.
Extraction of Haliclona manglaris lectin
The sponge Haliclona manglaris is cut into small pieces with a knife and then placed in a mortar and pestle and ground into powder. Next, the Haliclona manglaris powder is homogenized by placing it in a buffer solution and the whole mixture is filtered. The resulting filtrate is centrifuged at a low temperature and the supernatant is collected at the end to obtain the crude lectin.
Purification of Haliclona manglaris lectin
We offer column chromatography to purify Haliclona manglaris lectin, where the crude extract is upsampled onto a chromatography column, rinsed with a specific buffer of choice to remove the remaining protein impurities, and eluted with tris buffered saline (TBS) solution. The grades eluted with TBS solution are collected, dialyzed with distilled water, and freeze-dried to obtain pure Haliclona manglaris lectin.
Characterization of Haliclona manglaris lectin
- Structural characterization
Haliclona manglaris lectin is a glycan-binding protein, and we offer gas chromatography (GC), liquid chromatography (LC), infrared spectroscopy (IR), nuclear magnetic resonance (NMR), and mass spectrometry (MS) to comprehensively examine glycan linkage order, glycan composition, and glycan conformation.
We also offer Circular Dichroism (CD) spectroscopy to test its secondary structure.
- Molecular weight assay
- The molecular weight of lectins under denaturing conditions is determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and stained with Coomassie Brilliant Blue.
- The natural molecular weight of the lectins is detected by size exclusion chromatography (SEC).
- The average molecular weight of lectins is measured with electrospray ionization MS (ESI-MS).
The haemagglutination activity of Haliclona manglaris lectin is measured on a microtitre plate using a two-fold serial dilution method. Saline, diluted lectin, and erythrocyte suspension are added sequentially to the wells of the microtitre plate and the ability of lectin to agglutinate erythrocytes is observed under the microscope.
In addition, we also use this method to investigate the effect of different temperatures and pH on the stability of the lectin.
- The effect of temperature on lectin stability is assessed by incubating Haliclona manglaris lectin at different temperatures for the same amount of time and then performing a haemagglutination activity assay.
- The effect of pH on lectin stability is assessed by serially diluting Haliclona manglaris lectin in different buffers and incubating it for the same amount of time followed by a haemagglutination activity assay.
The internal sequence of Haliclona manglaris lectin is determined by tandem MS (MS/MS). The experimental sequence is shown below:
- SDS-PAGE is performed on Haliclona manglaris lectin
- Decolorization, reduction, and carbamidomethylation of Haliclona manglaris lectin
- Digestion of Haliclona manglaris lectin with protease
- Concentrate the peptide from Haliclona manglaris lectin
- Concentrated peptides are sequenced by MS/MS
Applications
- Haliclona manglaris lectin recognizes glycans on bacterial membranes and can inhibit biofilm formation and prevent infections caused by bacterial biofilms.
- Haliclona manglaris lectin reversibly binds to cell surface glycans without altering their structure, so it can be used to recognize cells.
- Haliclona manglaris lectin has enhanced immunomodulatory, anti-inflammatory, anti-tumor, and anti-viral activities, and it can be used in nutraceuticals and drug research and development.
Advantages
- We have a professional marine biomolecule research team, which provides excellent Haliclona manglaris lectin production service and high-quality lectin products according to the client's research needs.
- Our Haliclona manglaris lectin production services apply to food processing, drug discovery, health product development, and many other fields.
- We provide Haliclona manglaris lectin extraction, purification, and characterization in a one-stop service, which greatly saves clients' time and meets their needs.
Frequently Asked Questions
What are the characteristics of sponge lectins?
Sponge lectins possess the basic characteristics of the presence of a carbohydrate recognition structural domain and are not catalytically active. At the same time, the number of subunits of lectins from sponges varies greatly, from monomers to octamers. In addition, sponge lectins are characterized by their ability to remain active at high temperatures.
What marine organisms are marine lectins found in?
Marine lectins are a group of proteins with agglutinating activity that are found in many marine organisms. Some notable marine organisms include seaweeds, sponges, corals, mollusks, and cnidarians. The marine lectins produced by these organisms are believed to have biological activities such as antibacterial, antiviral, antitumor, antithrombotic, and wound healing.
As a professional glycobiology company, CD BioGlyco offers a comprehensive range of marine biomolecule production services to our clients, with highly skilled biology and extensive expertise. Marine lectins are a versatile class of biomolecules, and our dedicated service team extracts high-quality lectins from a wide range of marine organisms to meet our client's specific needs. Please feel free to contact us if you would like to know more about marine lectins.
Reference
- Gomes Filho, S.M.; et al. Marine sponge lectins: actual status on properties and biological activities. Molecules. 2015, 20(1): 348-357.
