Unleashing the Power of Hemagglutinin-Precision in Every Production
As an attachment factor and membrane fusion protein, hemagglutinin is a glycoprotein with a multifunctional function. It is a key molecule in mediating viral entry into the cell and is an important target for neutralizing antibodies as well as vaccine development. In addition, haemagglutinin is a type of lectin due to its haemagglutinating activity. Since marine sponges are an important source of haemagglutinin, CD BioGlyco offers a custom hemagglutinin production service based on our extensive experience in the Production of Sponge-derived Lectins.
Extraction of hemagglutinin
Hemagglutinin from marine organisms is generally extracted by organic solvent precipitation. After the cells are broken and centrifuged, the supernatant is taken and organic reagents are added to it, followed by filtration and freeze-drying of the resulting precipitate to obtain a crude hemagglutinin product.
Isolation and purification of hemagglutinin
Purification of hemagglutinin is usually carried out by column chromatography. We collect the precipitate after salting out the crude hemagglutinin product for extraction. Next, the precipitate is dissolved with buffer and subjected to ion exchange and gel filtration chromatography. Finally, high-purity hemagglutinin is obtained by collecting and concentrating the eluate containing the hemagglutinin fraction.
The purity of the obtained hemagglutinin is identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Characterization of hemagglutinin
Hemagglutinin is a biomolecule and molecular weight is determined by SDS-PAGE, gel filtration, vapor pressure, and MS.
- Haemagglutinating activity
The haemagglutinating activity of hemagglutinin against different organisms can be observed under the microscope after adding diluted hemagglutinin and erythrocyte suspensions from different sources to the microtiter plate.
In addition, the sugar-binding property of hemagglutinin can be detected by observing the agglutination of erythrocytes on the microtiter plate when different sugar solutions are added to it.
Publication Data
Technology: Electrochemical impedance spectroscopy (EIS) and fluorescence lifetime correlation spectroscopy (FLCS)
Journal: Frontiers in Molecular Biosciences
IF: 5
Published: 2022
Results: In this article, the authors characterized the degree of binding of the trimeric protein hemagglutinin to the host using EIS and FLCS. The authors also studied the inhibitory effect of salivary acid inhibitors on the binding of hemagglutinin to the host mimetic surface and observed that although all inhibitors were inhibitory, they did not prevent host binding.
Fig.1 Effects of haemagglutinin on membrane capacitance (A) and resistivity (B) generation in cell membranes. (Roy, et al., 2022)
Applications
- Hemagglutinin can bind to salivary acid-containing receptors on cells, so it can be used to recognize target vertebrate cells.
- Hemagglutinin is a major target for neutralizing antibodies and can be used to develop universal vaccines that produce reproducible antibodies.
- Hemagglutinin binds specifically to A or B blood group surface antigens on red blood cells, allowing small blood samples to be tested and an individual's ABO blood group determined.
Advantages
- With years of experience in Biomolecule Production, we offer large-scale production of hemagglutinin service and haemagglutinin products.
- Our R&D team has mastered the advanced technology of Marine Lectin Production, and we provide a wide range of hemagglutinin production solutions according to our client's special requirements.
- Our solutions cover the entire process of hemagglutinin extraction, purification, and analysis, and help our clients to solve the problems encountered in their research and development projects at high speed.
Frequently Asked Questions
Is hemagglutinin a toxin?
Hemagglutinin is one of the non-toxic ingredients that possesses the carbohydrate-binding activity of lectins and E-adhesin-binding activity. These activities promote intestinal activity.
Is hemagglutinin a glycoprotein?
Hemagglutinins are a trimer of the same three proteins and are homotrimeric glycoproteins, which appear on the surface of influenza viruses and bind the virus to infected cells.
CD BioGlyco is a leading global biotechnology company providing one-stop marine biomolecule production services. Our professional R&D team consists of biologists who tailor the production solutions to the specific needs of our clients, helping them to complete their projects quickly. Please feel free to contact us for more information.
References
- Roy, A.; et al. A cell-free biomembrane platform for multimodal study of influenza virus hemagglutinin and for evaluation of entry-inhibitors against hemagglutinin. Front. Mol. Biosci. 2022, 9: 1017338.
