Bridging Science and Innovation with Cinachyrella apion Lectin Production
Marine sponges are a rich source of lectins, and Sponge-derived Lectins tend to promote cell recognition and adhesion. One of these lectins, from the sponge Cinachyrella apion, cross-reacts with polyclonal antibodies and has strong haemagglutinating activity against human erythrocytes. CD BioGlyco has a long history of working with various types of Marine Biomolecule Production and has applied these biomolecules to many areas of research. Here, with our skilled and advanced Marine Lectin Production skills, we provide a one-stop Cinachyrella apion lectin production solution to our clients.
Extraction of Cinachyrella apion lectin
Cinachyrella apion is crushed and the buffer is added to it at room temperature and centrifuged at high speed. Crude extract of lectin is obtained by precipitating the supernatant with organic reagents and freeze-drying.
Isolation and purification of Cinachyrella apion lectin
We use an affinity chromatography column to purify Cinachyrella apion lectin.
- The extracted crude lectin is loaded into a chromatographic column, the column is rinsed with eluent and the eluent is collected based on the appearance and disappearance of peaks representing the protein in the graph.
- The eluate is then dialyzed with distilled water to remove NaCl.
- Finally, it is concentrated and freeze-dried to obtain the lectin pure product.
Analysis of Cinachyrella apion lectin
The purity of lectins is examined by high-performance liquid chromatography (HPLC) and polyacrylamide gel electrophoresis (PAGE).
- Molecular weight and subunit molecular weight determination
The molecular weight and subunit molecular weight of lectin is determined using gel permeation chromatography (GPC) and sodium dodecyl sulfate PAGE (SDS-PAGE).
- Determination of clotting activity
The agglutinin is diluted in saline and added to erythrocyte suspension, the agglutination effect of this agglutinin is observed under the microscope.
- Sugar inhibitory activity
Different types of sugar solutions and the same erythrocyte suspension are added to the diluted lectin, and the inhibitory activity of different sugars on the lectin is observed under the microscope.
Publication Data
Technology: Flow cytometry and protein blot analysis
Journal: Marine Drugs
IF: 5.4
Published: 2012
Results: In this research, the authors extracted a lectin from the sponge Cinachyrella apion with good biological activity and examined its properties. The lectin has a molecular weight of 124 kDa and consists of eight 15.5 kDa subunits assembled by non-covalent interactions. The lectin is heat-resistant between 0-60°C and pH-stable. The lectin was found to be non-toxic to cells by flow cytometry and protein blotting analysis and possessed strong haemagglutinating activity, preferentially agglutinating papainized type A erythrocytes. In this research, the authors also showed that Cinachyrella apion lectin can inhibit the proliferation of cultured tumor cell lines by inducing cell death.
Fig.1 Cytotoxicity testing of Cinachyrella apion lectins against HeLa tumour lines. (Rabelo, et al., 2012)
Applications
- Cinachyrella apion lectin has anti-fungal and anti-insect activity, so it can be used to control pathogens.
- Cinachyrella apion lectin can be used to develop antitumor and antiviral pharmaceuticals.
- Cinachyrella apion lectins are used in a variety of biological fields, including cell separation, identification of blood groups and microorganisms, and monitoring changes on the surface of normal and tumor cells.
Advantages
- Our service team has a deep background in marine biomolecule research, ensuring that we provide our clients with quality Cinachyrella apion lectin production solutions and high-purity lectin products.
- We offer a comprehensive range of services including Cinachyrella apion lectin production, purification, characterization of physicochemical properties, and bioactivity analysis.
- With an in-depth understanding of structure-function relationships, we assist our clients in the design and optimization of Cinachyrella apion lectin to enhance their biological activity and safety.
Frequently Asked Questions
What are the biological activities of Cinachyrella apion lectin?
Cinachyrella apion lectin has a variety of biological activities, including antitumor, immunomodulatory, and anti-HIV-1 reverse transcriptase inhibitory activities, which can be considered antipathogenic activities.
Why are lectins toxic?
Some types of lectins can bind to the digestive system walls, which can lead to serious problems. For example, phytohemagglutinins can cause severe abdominal pain, vomiting, and diarrhea.
CD BioGlyco has many years of experience producing lectins from marine sponges using advanced technology. We not only perform meticulous extraction but also fully and comprehensively characterize Cinachyrella apion lectin. We offer Cinachyrella apion lectin production solutions to various industries for their R&D needs. If you have any questions, please feel free to contact us and our staff will respond promptly.
Reference
- Rabelo, L.; et al. A lactose-binding lectin from the marine sponge Cinachyrella apion (Cal) induces cell death in human cervical adenocarcinoma cells. Marine Drugs. 2012, 10(4): 727-743.
