Technology-led High-efficiency Mangrove Polysaccharide Production
Mangrove is a Plant that inhabits the marine ecosystem. It is also a highly productive and diverse ecosystem, rich in many bioactive substances. Mangrove polysaccharide is a widespread Marine Carbohydrate. Depending on the needs of our clients, our experienced staff provides mangrove polysaccharide production services from different sources. Whether you need mangrove sediment polysaccharide, mangrove-associated microbial polysaccharide, or mangrove-associated organismal polysaccharide, we provide you with the most satisfactory production solution. Moreover, our lab provides custom Marine Biomolecule Production services to clients.
Extraction and purification of mangrove polysaccharide
- For sedimentary, microbial, and biogenic polysaccharides, our lab offers different extraction strategies to meet the high yield of mangrove polysaccharides production. For microbial polysaccharides, we perform polysaccharide extraction by mycobacterial pulverization, hot water extraction, alcohol precipitation, centrifugal drying, and deproteinization by the Sevage method. For plant polysaccharides, we offer high-quality hot water extraction, ultrasonic extraction, and Enzymatic Extraction.
- For the collected crude polysaccharides, we further provide analytical purification services using Chromatography Purification, Precipitation Purification, or Ultracentrifugation Purification.
Characterization and bioactivity analysis of mangrove polysaccharide
- Our lab provides accurate structural characterization of mangrove polysaccharides by tandem mass spectrometry (MS/MS), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and X-ray. Our researchers are experienced in molecular weight determination of polysaccharides, viscosity measurements, glycosidic linkage analyses, and modifications of polysaccharides (e.g., methylation analyses).
- We have a reliable bioactivity team to help our clients test various activities of mangrove polysaccharides such as antioxidant activity, anti-tumor activity, anti-inflammatory activity, etc.
Publication
Technology: FTIR, HPLC, Ultrasonic extraction
Journal: Proceedings
Published: 2021
Results: In this article, researchers used ultrasonic extraction to extract polysaccharides from different marine species (Ulva lactuca, Jania rubens, and the marine plant mangrove Avicennia marina) and analyze their structure and biological activity. The results showed that the polysaccharides from mangroves had the highest carbohydrate content and the strongest antioxidant activity of about 44 ± 1%. This study promotes the study of the antioxidant mechanism of sulfated polysaccharides and the extraction of mangrove polysaccharides.
Fig.1 Indicator assessment of polysaccharides from different sources. (Essa, et al., 2021)
Applications
- Mangrove polysaccharides can be used in the development of natural antioxidants.
- Mangrove polysaccharide plays an important role in the study of the mechanism of herpes simplex virus-1 (HSV-1) resistance in vitro.
- Mangrove polysaccharides can be used in the development of functional food and drugs.
Advantages of Us
- CD BioGlyco has a well-trained carbohydrate production team to ensure efficient and high-yield production of mangrove polysaccharides.
- Our experienced extraction staff uses orthogonal and response surface experiments to optimize the entire extraction process.
- The strategies for extraction and purification we provide effectively remove impurities and increase purity.
Frequently Asked Questions
What is the mangrove ecosystem?
Mangrove forests are special forest plants growing on tropical and subtropical coastal mudflats, which are special ecosystems in the transition from land to sea. It is both marine and terrestrial and is rich in unique fungal resources. Mangrove symbiotic microorganisms produce many medicinally active secondary metabolites. Mangrove sediments are rich in carbohydrates and microorganisms.
What is the principle behind the separation of polysaccharides by gel column chromatography?
Gel column chromatography separates polysaccharide molecules based on the size and shape of the polysaccharide, i.e. the molecular sieve principle. A mixture of polysaccharides is added to a column filled with gel particles and eluted with buffer. Small molecules enter and exit the gel particles freely and form a dynamic equilibrium in the mobile and stationary phases and therefore flow out slowly. Large molecules cannot enter the stationary phase in the gel particles and therefore flow out of the mobile phase quickly.
CD BioGlyco is committed to providing our clients with the best solution for mangrove polysaccharide production. Our lab provides different production strategies for different sources of polysaccharides. For any further information, do not hesitate to contact us.
Reference
- Essa, H.L.; et al. Ultrasonically-extracted marine polysaccharides as potential green antioxidant alternatives. Proceedings. 2021, 67(1).
