Expanding Production of Sulfated Polysaccharides (SP) through Professional Services
Marine ecosystems are a rich source of Marine Biomolecule Production. Seaweeds are rich in carbohydrates, especially natural bioactive SP, which is a natural polysaccharide containing sulfate groups and is widely found in Marine Plants. SP is mainly found in seaweeds in the form of fucoidan, fucoidan, carrageenan, and chlorella sulfated polysaccharides. CD BioGlyco provides pre-treatment, extraction, isolation, purification, characterization, and activity analysis of many types of sulfated polysaccharides. Moreover, our lab provides various Marine Carbohydrate Production services to clients.
SP pre-treatment
CD BioGlyco provides satisfactory marine plant polysaccharide pretreatment services including material selection and impurity removal, sample drying, and fat and color removal.
- According to different raw materials, our experienced staff provides the most suitable decontamination and drying services such as water washing and decontamination, natural drying, drying, freeze drying, and other services.
- We provide professional fat and pigment impurity removal services by rope extraction, organic solvent extraction, and supercritical extraction.
SP extraction and purification
Our lab provides high-yield SP extractions by hot water extraction (80~100°C), Enzymatic Extraction, Assisted Extraction, or Supercritical Fluid Extraction. Without destroying the molecular structure and functional groups of the polysaccharides, our lab improves the yield and purity of the polysaccharides by the addition of calcium-chelating agents or acids.
Our lab provides high-purity SP separation services by high-performance liquid chromatography (HPLC), Ultracentrifugation, ion exchange chromatography, and gel filtration.
SP characterization
Relying on state-of-the-art analytical systems and experienced operators, we offer characterization services for single-component or mixture SPs. We provide SP molecular weight determination, structural characterization, linkage analysis, degree of sulfation analysis, sulfate-based site analysis, and conformation analysis services.
CD BioGlyco provides a variety of sulfated polysaccharide production services including but not limited to:
- Sulfated Galactan Production
- Sulfated Fucan Production
- Dictyota mertensii Sulfate Fucan Production
- Lobophora variegata Sulfate Fucan Production
- Spatoglossum schroederi Sulfate Fucan Production
- Fucus vesiculosus Sulfate Fucan Production
- Sulfated Algae Polysaccharide Production
- Sargassum tenerrimum Sulfated Algae Polysaccharide
- Sargassum wightii Sulfated Algae Polysaccharide
- Turbinaria conoides Sulfated Algae Polysaccharide
- Turbinaria ornata Sulfated Algae Polysaccharide
- Padina tetrastromatica Sulfated Algae Polysaccharide
- Sulfated Heterorhamnans Production
- Sulfated Homopolysaccharide Production
- Sulfated Heteropolysaccharide Production
- Other Sulfate Polysaccharide Production
- Sargassum swartzii Sulfated Polysaccharide
- Scinaia hatei Sulfated Polysaccharide
- Laminaria japonica Sulfated Polysaccharide
- Sargassum tenerrimum Sulfated Polysaccharide
- Sargassum wightii Sulfated Polysaccharide
- Turbinaria conoides Sulfated Polysaccharide
- Turbinaria ornata Sulfated Polysaccharide
- Padina tetrastromatica Sulfated Polysaccharide
- Sargassum wightii Sulfated Polysaccharide
- Halymenia floresia Sulfated Polysaccharide
- Asparagopsis taxiformis Sulfated Polysaccharide
- Sargassum tenerrimum Sulfated Polysaccharide
- Laminaria cichorioides Sulfated Polysaccharide
- Cryptonemia crenulata Sulfated Polysaccharide
- Acaudina molpadioidea Sulfated Polysaccharide
- Asparagopsis taxiformis Sulfated Polysaccharide
- Lomentaria catenata Sulfated Polysaccharide
- Caulerpa cupressoides var. lycopodium Sulfated Polysaccharide
Publication
Technology: Enzymatic extraction, Ion-exchange chromatographic fractionation, FTIR spectroscopy, HPLC
Journal: Molecules
Published: 2019
IF: 4.6
Results: In this study, the researchers used enzymatic hydrolysis and ion-exchange chromatographic fractionation combined for the extraction of high molecular weight SP. The researchers determined the degree of polymerization, carbohydrate, protein, and sulfate content, and antioxidant and antitumor activities of each of the eight fractions (F1-F8) obtained by ion-exchange chromatographic separation. The analysis revealed that the antioxidant and antitumor activities of fractions F4, F5, and F8 were more pronounced. These data provide valuable information for the study of the effects of the degree of polymerization of SP, protein content, sugar composition, variations, and the type of functional groups on the bioactivities.
Fig.1 Antioxidant activities of the column effluent fractions at different concentrations. (Abou El Azm, et al., 2019)
Applications
- SP can be used in the research of drug delivery, wound healing, and regenerative medicine.
- SP is an important component in the development of marine polysaccharide drugs.
- SP activates signaling pathways in plant cells and is effective in defense against plant pathogens.
- SP can be used as a thickener, stabilizer, emulsifier, swelling agent, and gelling agent in food industry research.
- SP plays a key role in immunomodulation, neuroprotection, anti-lipid, anti-tumor, anti-diabetic, anti-oxidant, and anti-inflammatory.
Advantages of Us
- The supercritical extraction service we provide not only minimizes the impact on the properties of polysaccharides but also leaves no organic solvent residue.
- Our freeze-drying services protect the structure and properties of natural polysaccharides to an extraordinary degree, facilitating the acquisition of high-viscosity and high molecular mass polysaccharides.
- Our professional polysaccharide production team has rich operational experience to provide satisfactory SP production services according to clients' needs.
Frequently Asked Questions
What are some of the common seaweeds used for SP production?
Based on morphological pigmentation features, seaweeds can be divided into three main sections:
- Rhodophyta (red algae) mainly includes Gracilaria, Porphyra, and Eucheuma.
- Chlorophyta (green algae) mainly includes Monostroma, Caulerpa, Chlorella, and Ulva.
- Phaeophyceae (brown algae) mainly include Laminariaceae, Sargassum, and Alariaceae.
Why are polysaccharides degreased before extraction?
Degreasing mainly removes oils from plant cells, which surround the cells and hinder the release of polysaccharides. In addition, degreasing helps to remove other impurities, such as proteins and small molecules, which improves the purity of polysaccharides.
CD BioGlyco has experienced specialists with a strong background in glycobiology to solve all challenges for our clients. We are committed to being your first choice in the production of SP. Please don't hesitate to contact us whenever you need assistance.
Reference
- Abou El Azm, N.; et al. Production of bioactive compounds from the sulfated polysaccharides extracts of Ulva lactuca: Post-extraction enzymatic hydrolysis followed by ion-exchange chromatographic fractionation. Molecules. 2019, 24(11): 2132.
