Treasures of the Deep: Exploring the Marine Nigricanoside Process
Based on a professional Marine Biomolecule Production team, CD BioGlyco provides various Marine Glycolipid Production such as Marine Glycosphingolipid Production, Marine Glycoglycerolipid Production, and Marine Atypical Glycolipid Production services. Nigricanoside is a special glycolipid with antimitotic activity. Relying on selected raw material resources and through high-level extraction and purification technologies, we provide high-purity and high-efficiency nigricanoside.
Extraction and Purification
Nigricanoside mainly comes from the green alga Avrainvillea nigricans. Its activity is closely related to extraction and separation technology, and our professional researchers provide you with reliable services.
- Organic solvent extraction: We use methanol to thoroughly extract the active molecules. The methanol-soluble fraction is partitioned using water and ethyl acetate, and the ethyl acetate-soluble fraction is further partitioned using an organic mixing reagent. Importantly, biologically active target molecules are methylated with trimethylsilyl diazomethane before isolation.
- Chromatography purification: For methylated samples, we provide high-quality separation services using normal-phase flash chromatography, Sephadex chromatography, and reversed-phase high-performance liquid chromatography (HPLC). All separation gradients, buffer concentrations, and column packing selections have been optimized by professionals.
Combining multiple analytical tools, we provide comprehensive nigricanoside structural characterization services. This not only helps reveal its functions in living organisms but also promotes its application in fields such as biopharmaceuticals.
- High-resolution electrospray ionization mass spectroscopy (HRESIMS)analysis: We use MS to determine the molecular weight and structural fragment information of nigricanoside.
- Cryoprobe-assisted nuclear magnetic resonance (NMR) spectroscopy analysis: Through one-dimensional and two-dimensional spectral information, we provide a series of services such as connection mode, saturation, side chain position, etc.
Publication Data
Journal: Marine Drugs
Published: 2018
IF: 4.9
Results: In this study, the researchers discuss the natural source of nigricanoside and the synthesized nigricanoside. The natural source of nigricanoside is very rare. With 28 kg of wet material isolating approximately a few hundred micrograms of nigricanoside and the methyl esters of nigricanosides. The researchers analyzed the synthetic nigricanoside for its lack of strong biological activity. A plausible explanation is the presence of an unknown product in the fractions corresponding to natural Nigricanoside as the molecule responsible for the antitumor activity exhibited by these natural products.
Fig.1 Structures of nigricanosides A and B. (Cheng-Sánchez & Sarabia, 2018)
Applications
- Nigricanoside has strong antimitotic activity and plays a key role in promoting tubulin polymerization.
- Nigricanoside is a natural marine product that promotes the development of marine drugs.
- Nigricanoside with a clear production structure is conducive to promoting the development of glycolipid structure-activity relationship.
Advantages
- According to client needs, we provide customized nigricanoside production solutions.
- Our well-trained production team ensures efficient production of nigricanosides based on advanced production technology.
- Our researchers support clients by providing raw materials for sample extraction.
Frequently Asked Questions
What is the molecular formula and molecular weight of nigricanoside A and B?
The molecular formula of nigricanoside A is C45H76O16 and its molecular weight is 873.075 Da. The molecular formula of nigricanoside B is C45H74O16 and its molecular weight is 871.06 Da.
The molecular
Compared with other biological samples, the extraction rate of nigricanoside is lower. Why choose naturally originated nigricanosides?
The natural extraction method avoids toxic chemical reagents and complex synthesis processes. Nigricanoside has a complex, asymmetric structure. Most importantly, naturally extracted nigricanoside has strong biological activity.
CD BioGlyco has experienced production experts to provide clients with high-purity nigricanoside production services. We are committed to meeting all of our client's needs in the production of marine glycolipids. In addition to basic extraction and separation, we also provide professional testing services such as structural characterization, molecular weight testing, activity analysis, etc. If you want to know more about the production of nigricanoside, please feel free to contact us.
Reference
- Cheng-Sánchez, I.; Sarabia, F. Chemistry and biology of bioactive glycolipids of marine origin. Marine Drugs. 2018, 16(9): 294.
