Crafting Excellence in Bartoloside Production Services
Bartolosides, a class of glycolipids derived from marine cyanobacteria, possess a distinctive structure characterized by a core dialkylresorcinol portion adorned with one or two xylose units and an aliphatic chain containing chlorine atoms. Some bartolosides also feature a C-glycosyl portion. The anti-inflammatory and anticancer properties of bartolosides have attracted growing interest among researchers. CD BioGlyco leverages our extensive Marine Glycolipid Production experience to provide a comprehensive Marine Atypical Glycolipid Production Service for our clients, encompassing our professional bartoloside production service.
Pretreatment
We gather and purify newly harvested cyanobacterial samples to eliminate any impurities and contaminants that may be present on the surface. The cleaned cyanobacterial samples are then dried using low-temperature drying, and the dried cyanobacterial samples are subsequently pulverized to make it easier to extract the active substances.
Bartoloside Extraction
- We utilize organic solvents such as methanol, ethanol, and dimethyl sulfoxide for efficient extraction of active compounds from algal samples. The process involves immersing the samples in the solvent to facilitate the dissolution of the desired compounds. Once dissolved, the solution is then subjected to separation techniques such as filtration or centrifugation for isolation and purification of the active substances.
- We immerse samples in water, utilize hot water to expedite the dissolution of water-soluble active compounds, and subsequently isolate the active compounds from the solution through processes such as filtration or centrifugation.
- Our experts harness the mechanical and thermal effects of ultrasound to enhance the release of active substances from cyanobacteria, thereby improving extraction efficiency and speed.
Bartoloside Characterization
We employ techniques such as high-performance liquid chromatography (HPLC) to separate the products and utilize nuclear magnetic resonance (NMR) and mass spectrometry (MS) to determine the molecular structure and chemical composition. Additionally, we employ other techniques such as infrared spectroscopy (IR) and ultraviolet-visible spectroscopy (UV-Vis), as appropriate, for further product characterization.
Publication Data
Journal: Molecules
Published: 2022
IF: 4.2
Results: This article discusses the possibility of using secondary metabolites from marine cyanobacteria for cancer treatment. Marine cyanobacteria are an important source of bioactive molecules and they produce a wide chemical diversity of metabolites including glycolipids, macrolides, peptides, and polyketides. Many of these metabolites have been isolated and tested and have shown important biological activities such as cytotoxicity, antitumor, and antiproliferative activities. Among these, the article points to bartolosides, a class of chlorinated aromatic-based glycolipids found in marine cyanobacteria. These compounds showed mild cytotoxicity when evaluated against three cancer cell lines. Among these compounds, bartoloside A demonstrated IC50 values of 22 µM against MG-63 cells, 40 µM against RKO cells, and 23 µM against T-47D cells. Similarly, bartoloside E exhibited IC50 values of 39 µM against MG-63 cells, 40 µM against RKO cells, and 22 µM against T-47D cells. Additionally, exposure of the T-47D cell line to bartoloside I resulted in an IC50 value of 59.8 µM for cytotoxic effect.
Fig.1 Structure of bartoloside A, B, and I. (Robles-Bañuelos, et al., 2022)
Applications
- Due to its antioxidant and anti-inflammatory properties, bartoloside can be used in the development of skincare and cosmetic products to help protect the skin from environmental stresses and free radicals.
- Bartoloside exhibits anticancer and antioxidant properties, making it a potential drug candidate used in research on inflammatory diseases.
- Bartoloside has inhibitory activity against plant pathogenic bacteria and insects and can be used in the development of plant protection agents.
Advantages
- Our experts tailor production to align with the requirements of our clients, thereby enhancing productivity and output.
- We have established partnerships with some research institutes and companies, which allows us to fully integrate resources from all parties to improve productivity and reduce costs.
- We have a professional research team with a rich biotechnology background and practical experience to carry out the production of bartoloside efficiently.
Frequently Asked Questions
What are the toxicology studies on bartoloside?
Bartolosides are characterized in anticancer drug discovery by the fact that they are a class of compounds with a halo-alkylresorcinols structure that possesses some cytotoxic activity. Among them, bartoloside A and bartoloside B show low cytotoxic effects and have some inhibitory effects on certain cancer cell lines. However, bartoloside C and other congeners showed weaker cytotoxic effects.
How do you ensure the purity and quality of bartoloside extracted from marine plants?
Ensuring the purity and quality of bartoloside extracted from marine plants usually requires a series of technical and control measures. These include, but are not limited to, the selection of suitable marine plants, optimization of extraction methods, and stringent separation and purification procedures.
CD BioGlyco provides a comprehensive bartoloside production service, encompassing the entire process from extraction to product characterization. If you are interested in our bartoloside production service, please don't hesitate to contact us!
Reference
- Robles-Bañuelos, B.; et al. Marine cyanobacteria as sources of lead anticancer compounds: A review of families of metabolites with cytotoxic, antiproliferative, and antineoplastic effects. Molecules. 2022, 27(15): 4814.
