Precision Production for Cutting-Edge Simplexide Solutions
Simplexide is a novel Atypical Glycolipid compound isolated from marine sponges that shows promising anti-cancer activity. At CD BioGlyco, our dedicated Marine Biomolecule Production team is skilled in the production process of all types of Glycolipids of marine biological origin, offering custom simplexide production services to our clients.
Extraction of Simplexide
We use methanol and trichloromethane as extraction solutions to extract simplexide sequentially from marine sponges. After the extraction is complete, all the extracts are combined and partitioned between water and n-BuOH. Then, the organic phase is collected and the solvent is removed under vacuum to obtain the crude extract of simplexide.
Purification of Simplexide
Simplexide is purified sequentially using reversed-phase column chromatography and direct column chromatography. Throughout the purification process, a thin-layer chromatographic (TLC) is used to check whether impurities are effectively removed. The purity of the obtained simplexide is tested by nuclear magnetic resonance hydrogen spectroscopy (1H NMR).
Characterization of Simplexide
- Structure
We offer NMR (1H NMR, 13C NMR), infrared (IR), and mass spectrometry (MS) techniques to characterize the structure of simplexide. The NMR techniques show the structure of simplexide, the type of glycosidic bonds, and how the bonds are connected, while the IR techniques show the number and position of the functional groups.
- Molecular Weight (Mw)
MS coupled with gel permeation chromatography (GPC) is an effective means of detecting the Mw of simplexide. This method can not only accurately test the molecular weight of simplexide, but also detect the molecular weight distribution.
In addition to the above-mentioned characterization, we also offer other angles of analysis, such as the biological activity of simplexide, according to the specific needs of our clients.
Applications
- Simplexide has shown inhibitory effects on a wide range of bacteria and viruses in research and can be used to develop new antibacterial and antiviral drugs.
- Simplexide can modulate the immune system and can be used in research for the treatment of diseases such as autoimmune diseases, inflammation, and other diseases associated with immune system dysregulation.
- As a glycolipid, simplexide can be used to construct biological membrane models to help researchers explore the structure and function of cell membranes and further understand cellular interactions and signaling.
Advantages
- We have efficient, safe, and reproducible simplexide production technology to provide clients with high-quality and high-purity simplexide products.
- We not only provide custom simplexide production services but also offer strong technical support to help clients solve problems encountered in the application process.
- We have an interdisciplinary team of chemists, biologists, and engineers with rich professional knowledge and experience to assist our clients in new simplexide production technology, and new product development.
Frequently Asked Questions
What is simplexide? And what are its structural features?
Simplexide is a glycolipid consisting of a long chain of secondary alcohols with a disaccharide chain structure of α-glucose and α-galactose. Natural simplexide is a mixture of homologs with alkyl chains of different lengths attached to a central -CHOH group. In addition, a large portion of the alkyl chain of simplexide has a methyl branching structure in the penultimate or penultimate third carbon atom.
What are the biological activities of simplexide?
Simplexide is a glycolipid molecule that, due to its unique structure and function, has a variety of potential biological activities, including immunomodulatory, anti-inflammatory, antibacterial, and antiviral, and synergistic effects with other biomolecules.
CD BioGlyco provides high-quality simplexide production services to ensure that products meet the highest standards of purity and activity. Please feel free to contact us if you are interested in the production of simplexide or other glycolipids, or if you would like more information.
Reference
- Cheng-Sánchez, I.; Sarabia, F. Chemistry and biology of bioactive glycolipids of marine origin. Marine Drugs. 2018, 16(9): 294.
