Empowering Wellness with Superior Roselipin Production
Roselipin is an Atypical Glycolipid from marine fungi with good antimicrobial properties. CD BioGlyco has a professional team dedicated to research on the Production of Marine Biomolecules, providing quality roselipin production solutions to our clients.
Extraction and Isolation of Roselipin
We use a solvent extraction method to extract and isolate roselipin from specific marine fungal strains.
In this method, we chose acetone as the extraction solvent and the multiple extraction method is used to ensure the extraction rate of roselipin. After extraction, all the extracts are combined and concentrated to obtain the roselipin crude extract.
Purification of Roselipin
We use high-performance liquid chromatography (HPLC) to purify roselipin. Based on the position of the characteristic peaks in the chromatogram and the intensity of the peaks, we accurately isolate roselipin from other non-glycolipid substances and obtain roselipin with high purity.
Characterization of Roselipin
- Structural Analysis
We use multiple types of nuclear magnetic resonance (NMR), infrared (IR) as well as ultraviolet spectroscopy (UV) to characterize the general structure of roselipin.
Among them, heteronuclear multiple quantum coherence (HMQC) spectra analyze the connection between protons and carbon, and 1H-detected heteronuclear multiple bond correlation (HMBC) spectra present information about the coupling of carbon and hydrogen atoms.
On the other hand, IR and UV spectra show us information about the specific groups contained in roselipin, including the type of group, the position of the linkage, and the conformation of the sugar ring, among others.
- Molecular Weight (Mw) Analysis
The relative Mw of roselipin is analyzed by gel permeation chromatography (GPC) and mass spectrometry (MS).
- Purity Analysis
The purity of roselipin is analysed by HPLC, i.e. the purity of roselipin is calculated from the area of the peaks in the spectrum.
- Molecular Formula Analysis
We provide the fast-atom-bombardment MS (FAB-MS) method to detect the molecular formula of the extract, which can further determine the accuracy of roselipin products.
Publication Data
Technology: NMR
Journal: Marine Drugs
IF: 4.9
Published: 2018
Results: In this article, the authors show the origin and biological activity of roselipin, which was isolated from the fungus Gliocladium roseum KF-1040 and tested by various NMR and found to have a unique structure in which a highly methylated fatty acid backbone is modified by mannose and arabinitol residues. Biological evaluation of roselipin revealed superior inhibitory activity against diacylglycerol acyltransferase. In addition, roselipin showed some antibacterial activity against Saccharomyces cervisiae and Aspergillus niger.
Fig.1 The structure of roselipin. (Cheng-Sánchez & Sarabia, 2018)
Applications
- Due to good antimicrobial properties, roselipin can be used to develop new antimicrobial agents.
- Roselipin can be used to construct nanoparticles or liposomes for delivery systems in drugs, vaccines, and gene therapy to enhance drug bioavailability and targeting.
- Roselipin displays anti-cancer properties and can inhibit the growth and spread of tumor cells. Therefore, roselipin can be used in anti-cancer research.
Advantages
- We have advanced chemical and biological extraction, purification, and characterization technologies to provide our clients with high-purity roselipin products.
- Our Glycolipid Production team provides tailor-made roselipin production solutions based on the client's needs, including personalization in terms of yield, purity, and specifications.
- We use high-end quality control systems, including HPLC, FAB-MS, and other analysis methods, to ensure that the purity and quality of roselipin products meet client standards.
Frequently Asked Questions
What are the main components and structure of roselipin?
Roselipin is a glycolipid molecule, and its main components include sugar and lipid molecules. On a structural level, these molecules are connected by specific chemical bonds to form roselipin.
How to ensure the quality of roselipin products?
Through strict quality control processes, including raw material inspection, production process monitoring, and finished product testing, we ensure that each batch of product meets quality standards.
In the field of roselipin production, CD BioGlyco stands out as the preferred partner of many clients due to our excellent technical capabilities and professional service team. Our services cover a wide range of roselipin production, characterization, and product quality testing to ensure that the roselipin produced meets the needs of our clients. Please feel free to contact us if you are interested in our roselipin production services.
Reference
- Cheng-Sánchez, I.; Sarabia, F. Chemistry and biology of bioactive glycolipids of marine origin. Marine Drugs. 2018, 16(9): 294.
