Unlocking Plaxyloside Potential with Precision and Expertise
Plaxyloside is a glycolipid from marine sponges, consisting of C35 linear polysome retinoid alcohol aglycone and a linear carbohydrate chain of 6 β-xylopyranose units. At CD BioGlyco, we are dedicated to research on the Production of Marine Biomolecules, have mastered the advanced technology for the Production of Marine Glycolipids, and offer personalized solutions for the production of plaxyloside to clients all over the world.
Extraction
Firstly, we crush the sponge and then extract the cypress glycosides sequentially using methanol and chloroform. Next, all the extracts are combined and then distributed in specific solvents. Finally, the organic phase is collected and concentrated under a vacuum to obtain the crude cedrin (oil).
Purification
We use continuous column chromatography for the purification of plaxyloside. Firstly, plaxyloside is initially purified on silica gel column chromatography using CHCl3 as the eluent. Next, the plaxyloside-containing fractions are subjected to secondary purification on a SiO2 column, which removes non-glycolipid substances. After a series of purification operations, the plaxyloside-containing eluate is collected and the solvent is removed under vacuum to obtain pure plaxyloside (amorphous solid).
Characterization
The physicochemical properties of plaxyloside affect its function, we carry out the characterization of plaxyloside from multiple angles to help our clients gain a deeper understanding of plaxyloside.
- Rotativity: The rotativity of plaxyloside is analyzed using a rotameter.
- Molecular weight (Mw): Using gel permeation chromatography-mass spectrometry (GPC-MS) to analyze the Mw of plaxyloside.
- Structure: Nuclear magnetic resonance (NMR), infrared (IR), MS, and ultraviolet (UV) spectroscopy are used to analyze the structure of plaxyloside, including the way of glycan chain connection, sugar conformation, type of glycosidic bond, and so on.
Publication Data
Technology: NMR
Journal: Marine Drugs
IF: 4.9
Published: 2018
Results: In this article, the authors show the extraction method, as well as the structural features of plaxyloside. Plaxyloside was extracted and isolated from the sponge Plakortis simplex by researchers using solvent extraction, and its structure, was determined by chemical methods such as NMR. The results of structural analyses showed that plaxyloside is a glycolipid with linear polyisoprenoid alcohol aglycone and a linear carbohydrate chain of β-xylopyranose units. In addition, plaxyloside is the first naturally occurring oligosaccharide discovered that contains more than three (1→3) linkages.
Fig.1 The structure of plaxyloside. (Cheng-Sánchez & Sarabia, 2018)
Applications
- Plaxyloside can be used to develop functional food additives to enhance the nutritional value of foods.
- Plaxyloside inhibits the proliferation and spread of certain cancer cells. Therefore, it can be used to develop anti-cancer drugs.
- As a natural compound, plaxyloside can be used as a chemical lead in the development of other new drugs by structural optimization and functional modification.
Advantages
- Our team has mastered advanced marine glycolipid separation and extraction technology to ensure high purity and high yield of plaxyloside.
- We provide all-around technical support to our clients to help them solve problems encountered in the process of using plaxyloside.
- We provide custom plaxyloside solutions according to client's needs, including custom production and packaging services.
Frequently Asked Questions
What are the biological activities of plaxyloside?
Plaxyloside is an atypical glycolipid with a vast range of biological activities such as anti-virus, anti-cancer, anti-bacterial, immunomodulatory, and anti-inflammatory activities. These biological activities make plaxyloside potentially useful in a variety of medical and biotechnological applications, such as cancer therapy, antibacterial and antiviral drug development, immunomodulatory and anti-inflammatory drug discovery and development.
What types of marine glycolipid production services does CD BioGlyco offer?
CD BioGlyco offers a wide range of marine glycolipid production services to clients, including, but not limited to, the production of Marine Glycosphingolipid, Marine Glycoglycerolipid, Marine Atypical Glycolipid, and more.
CD BioGlyco provides one-stop plaxyloside production services to clients in various fields, i.e. from research and development to large-scale production and customized solutions. Our expertise covers extraction, purification, and characterization of marine biomolecules to ensure the high purity and activity of plaxyloside products. Please feel free to contact us if you are interested in our plaxyloside production services or have any questions, we look forward to working with you.
Reference
- Cheng-Sánchez, I.; Sarabia, F. Chemistry and biology of bioactive glycolipids of marine origin. Marine Drugs. 2018, 16(9): 294.
