Crafting Excellence in Erylusamide Production
CD BioGlyco utilizes advanced technology and years of experience in Marine Glycolipid Production to provide a full range of Marine Glycosphingolipid Production Services, Marine Glycoglycerolipid Production Services, and Marine Atypical Glycolipid Production Services. The details of our erylusamide production service are listed below.
Sample Pretreatment
Firstly, we select a suitable sample of marine organisms, with sponges typically chosen as the source for extracting erylusamide. The collected marine organism samples undergo preliminary treatment, including cleaning and removal of impurities. Subsequently, the samples are freeze-dried and ground with a grinder.
Sample Extraction
We extract the raw material with a solvent such as methanol at room temperature, and then the methanol extract is then extracted with dichloromethane. Subsequently, we subject a portion of the dichloromethane extract to crude separation and gradient elution using a chromatographic column and a suitable solvent to obtain the extracted isolate. Further separation is carried out by high-performance liquid chromatography (HPLC) to obtain erylusamides product. During the extraction process, we use microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), and other techniques to improve the extraction efficiency according to the actual situation. The rapid heating of the sample by microwave heating prompts the rapid reaction between the solvent and the target components, thus shortening the extraction time and enhancing the extraction effect. UAE utilizes the mechanical and chemical effects of ultrasound to break down the cell wall or increase the permeability of the substance, making it easier for the target components to be absorbed and released by the solvent.
Characterization
The structure of erylusamide is determined by one- and two-dimensional nuclear magnetic resonance (NMR) spectroscopy, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and chemical derivatization. Specifically, the structure of erylusamide is determined by analyzing its 1H and 13C NMR data and the molecular mass of erylusamide is determined by HR-ESI-MS analysis. Meanwhile, we further verify the functional groups of erylusamide, such as hydroxyl, ester, ketone, and amide, by chemical derivatization
Publication Data
Journal: Marine Drugs
Published: 2016
IF: 4.9
Results This article describes the discovery of novel atypical glycolipids found in a sponge called Erylus cf. deficiens, named erylusamides A-D. These compounds have a unique structure, including an oligosaccharide moiety consisting of five sugar residues and a never-before-seen long-chain dihydroxyketoamide. The article also describes the structural identification methods and the results of the analysis of the chemical derivatives of these compounds. The discovery of these novel glycolipids provides a potential source of drugs for marine organisms and may have anticancer activity.
Fig.1 The chemical structures of erylusamide A to D. (Gaspar, et al., 2016)
Applications
- Erylusamides have antibacterial, anti-inflammatory, antioxidant, and other biological activities and can be used as candidate compounds for drug discovery and development.
- Erylusamides are used in research as plant protectants in agriculture to help plants fight pests and diseases.
- Erylusamides can be used as tool compounds in chemical biology research to study metabolic pathways and signaling mechanisms in organisms, among others.
Advantages
- We provide customized production services according to the needs of clients, including different specifications, different purities, and different production quantities of erylusamides.
- We possess cutting-edge process facilities and production equipment to guarantee the efficiency and reliability of the production process.
- Our experts have mastered various biologics production techniques and technologies and can utilize advanced biotechnology platforms to efficiently produce atypical glycolipid compounds and erylusamides.
Frequently Asked Questions
What are erylusamides and what are their characteristics?
Erylusamides are a class of atypical glycolipid compounds with a specialized molecular structure. These compounds are originally isolated and characterized from the sponge genus Erylus, hence the name. The molecular structure of erylusamides contains unique sugar and fatty acyl groups, which make them different from traditional glycolipids.
How is the stability of erylusamides
Erylusamides exhibit stability at room temperature but are prone to decomposition in humid, light, and hot conditions. To ensure their stability, it is advisable to store erylusamides in a dry, cool, and light-protected environment while avoiding exposure to bright light.
CD BioGlyco is committed to efficiently producing erylusamides through optimized extraction processes while maintaining cost-effectiveness. Our dedication to providing courteous service and reliable work has earned us the trust of our clients. For further information, please do not hesitate to contact us.
Reference
- Gaspar, H.; et al. Erylusamides: Novel atypical glycolipids from Erylus cf. deficiens. Marine Drugs. 2016, 14(10): 179.
