Strengthening Safety Oversight to Ensure the Most Assured Chitosan Production
Under certain conditions, chitin can be partially deacetylated, and the chitin obtained with a deacetylation degree (DD) of more than 50% is called chitosan. It is a copolymer composed of glucosamine and N-acetyl glucosamine. Both chitin and chitosan are widely available polysaccharides from Marine Animals. Our high-quality Marine Carbohydrate Production team offers chitosan production in different molecular weights including high MW chitosans (HMWCs), medium MW chitosans (MMWCs), and low MW chitosans (LMWCs). Whether you want to produce Marine Biomolecule or Characterize Marine Carbohydrates, CD BioGlyco provides the most satisfactory solutions for you.
Natural resource chitosan production service
CD BioGlyco provides direct extraction of chitosan from marine animal sources. Our researchers use a combination of crush and puff (CP), acid solubilization, and alkaline precipitation to extract chitosan from animals.
We provide natural resource chitosan production including but not limited to shrimp chitosan production, lobster chitosan production, crabs chitosan production, prawns chitosan production, and squid chitosan production.
Chitin-based chitosan production service
Our laboratory offers chitosan preparation by deacetylation of chitin which consists of two steps:
- Chitin Extraction
- Deacetylation: We offer reliable deacetylation services through concentrated alkali heating methods, low alkali batch methods, one-pot methods, microwave-assisted heating methods, and enzymatic methods.
- Our experienced researchers provide chitosan preparation process optimization services by Doehlert design. Moreover, we provide DD testing services by potentiometric titration, acid-base titration, gas chromatography, infrared spectroscopy, or ultraviolet spectroscopy.
Chitosan derivative production service
Our lab provides two types of chitosan derivatives production services including N-acetyl-d-glucosamine (GlcNAc) production and glucosamine (GlcN) production service.
For the preparation of the collected crude samples, CD BioGlyco provides chitosan and its derivatives purification services, structural characterization services, and activity analysis services.
Chitosan and chitosan derivative purification
Our lab purifies the collected chitosan and its derivatives using high-performance liquid chromatography (HPLC).
Chitosan and chitosan derivative characterization
Our lab provides molecular weight distribution determination of chitosan by gel permeation chromatography coupled with multi-angle laser light scattering.
We characterize the structure of chitosan by X-ray diffraction, Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (NMR), and scanning electron microscopy (SEM).
Publication
Technology: FTIR, NMR, Morphology, Thermal analysis, Crystallinity index
Journal: Polymers
Published: 2022
IF: 5.0
Results: In this study, LWMC was prepared using a combination of formic acid and ultrasound. The optimal reaction conditions for depolymerization were obtained by Box-Behnken design and further structural characterization of LWMC was carried out. The analysis showed that the yield of LWMC was 89.398%, the molecular weight was 32.814 kDa and the degree of deacetylation was 98.076%.
Fig.1 NMR spectra of different chitosans. (Suryani, et al., 2022)
Applications
- Chitosan cationic polysaccharides and bioactives have promising applications as dietary fibers.
- Chitosan and its derivatives extend the storage time of food and can be used to develop bio-conservative films.
- Chitosan can be used as food additives such as thickeners, coating agents, clarifiers, antioxidants, flavor improvers, emulsifiers, etc.
Advantages of Us
- CD BioGlyco provides chitosan production service with different molecular weights according to the client's needs.
- Our experienced researchers offer a variety of methods for determining deacetylation to ensure the most accurate data is delivered.
- Our researchers strictly control the isolation and preparation conditions of chitosan to meet our clients' high-quality requirements.
Frequently Asked Questions
How to get chitosan derivatives?
Chitosan contains 3 reactive functional groups, 1 amino group, and 2 hydroxyl groups at the C6 and C3 positions. Chitosan derivatives are products obtained from the amino and hydroxyl groups of chitosan after a series of chemical modifications such as acylation, hydroxylation, etherification, alkylation, esterification, graft copolymerization, cross-linking, chelation and so on.
What is the chemical structure of chitosan?
Chitosan is a linear polysaccharide with a structure similar to that of cellulose, consisting of randomly distributed β-(1-4)-linked D-glucosamine (deacetyl unit) and N-acetyl-D-glucosamine (acetyl unit). The structural difference between chitosan and cellulose is that at carbon 2, the hydroxyl group of cellulose is replaced by an amino group.
What are the biological properties of chitosan?
Chitosan has strong biological properties including biocompatibility (safe, non-toxic, biodegradable), binding to mammalian and microbial cells, tissue regeneration, accelerated osteoclast formation, hemostasis, fungistatic, bactericidal, antitumor or anticancer, anticholesterol, central nervous system depressant, and immune adjuvant.
CD BioGlyco is constantly upgrading production processes and wants to be your premier partner in chitosan production. We have many years of experience in the production of marine polysaccharides to drive the project forward. Please feel free to contact us.
Reference
- Suryani, S.; et al. Production of low molecular weight chitosan using a combination of weak acid and ultrasonication methods. Polymers. 2022, 14(16): 3417.
